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However, the downstream events mediating the potential therapeutic effect of Wnt proteins are not fully understood. Previous studies have indicated that increased glycolysis is associated with osteoblast differentiation in response to Wnt signaling, but direct genetic evidence for the importance of glucose metabolism in Wnt\u2010induced bone formation is lacking. Here, we have generated compound transgenic mice to overexpress Wnt family member 7B (Wnt7b) transiently in the osteoblast lineage of postnatal mice, with or without concurrent deletion of the glucose transporter 1 (Glut1), also known as solute carrier family 2, facilitated glucose transporter member 1. Overexpression of Wnt7b in 1\u2010mo\u2010old mice for 1 wk markedly stimulated bone formation, but the effect was essentially abolished without Glut1, even though transient deletion of Glut1 itself did not affect normal bone accrual. Consistent with the\n                    <jats:italic>in vivo<\/jats:italic>\n                    results, Wnt7b increased Glut1 expression and glucose consumption in the primary culture of osteoblast lineage cells, and deletion of Glut1 diminished osteoblast differentiation\n                    <jats:italic>in vitro<\/jats:italic>\n                    . Thus, Wnt7b promotes bone formation in part through stimulating glucose metabolism in osteoblast lineage cells.\u2014Chen, H., Ji, X., Lee, W.\u2010C., Shi, Y., Li, B., Abel, E. D., Jiang, D., Huang, W., Long, F. Increased glycolysis mediates Wnt7b\u2010induced bone formation. FASEB J. 33, 7810\u20137821 (2019).\n                    <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/www.fasebj.org\">www.fasebj.org<\/jats:ext-link>\n                  <\/jats:p>","DOI":"10.1096\/fj.201900201rr","type":"journal-article","created":{"date-parts":[[2019,3,26]],"date-time":"2019-03-26T18:55:02Z","timestamp":1553626502000},"page":"7810-7821","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":65,"title":["Increased glycolysis mediates Wnt7b\u2010induced bone formation"],"prefix":"10.1096","volume":"33","author":[{"given":"Hong","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Orthopedics The First Affiliated Hospital of Chongqing Medical University  Chongqing China"},{"name":"Department of Orthopedic Surgery Washington University School of Medicine  St. Louis Missouri USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xing","family":"Ji","sequence":"additional","affiliation":[{"name":"Department of Orthopedic Surgery Washington University School of Medicine  St. Louis Missouri USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wen-Chih","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Orthopedic Surgery Washington University School of Medicine  St. Louis Missouri USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Shi","sequence":"additional","affiliation":[{"name":"Department of Orthopedic Surgery Washington University School of Medicine  St. Louis Missouri USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Boer","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Orthopedic Surgery Washington University School of Medicine  St. Louis Missouri USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dale","family":"Abel","sequence":"additional","affiliation":[{"name":"Division of Endocrinology and Metabolism Fraternal Order of Eagles Diabetes Research Center Carver College of Medicine University of Iowa  Iowa City Iowa USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dianming","family":"Jiang","sequence":"additional","affiliation":[{"name":"Department of Orthopedics The Third Affiliated Hospital of Chongqing Medical University  Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Orthopedics The First Affiliated Hospital of Chongqing Medical University  Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fanxin","family":"Long","sequence":"additional","affiliation":[{"name":"Department of Orthopedics The First Affiliated Hospital of Chongqing Medical University  Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,3,26]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/wdev.159"},{"key":"e_1_2_8_3_1","doi-asserted-by":"crossref","first-page":"513","DOI":"10.1016\/S0092-8674(01)00571-2","article-title":"LDL receptor-related protein 5 (LRP5) affects bone accrual and eye development","volume":"207","author":"Gong Y.","year":"2001","journal-title":"Cell"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa013444"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1086\/338450"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1136\/jmg.39.2.91"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1086\/318811"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1136\/jmedgenet-2013-101567"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajhg.2013.02.010"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1215458"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajhg.2013.02.009"},{"key":"e_1_2_8_12_1","doi-asserted-by":"crossref","first-page":"el002745","DOI":"10.1371\/journal.pgen.1002745","article-title":"WNT16 influences bone mineral density, cortical bone thickness, bone strength, and osteoporotic fracture risk","volume":"8","author":"Zheng H. 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