{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T11:19:29Z","timestamp":1790507969594,"version":"4.1.0"},"reference-count":68,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2019,10,1]],"date-time":"2019-10-01T00:00:00Z","timestamp":1569888000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2019,12]]},"abstract":"<jats:p>\n                    M3 is a broad\u2010spectrum chemokine\u2010binding protein that inactivates inflammatory chemokines, including CCL2, CCL5, and CX\n                    <jats:sub>3<\/jats:sub>\n                    CL1. The aim of this study was to compare whether M3 could inhibit angiogenesis driven by inflammation or ischemia. Here, apolipoprotein E\n                    <jats:sup>\u2013\/\u2013<\/jats:sup>\n                    mice were injected with adenoviral M3 (AdM3) or control adenoviral green fluorescent protein (AdGFP) 3 d prior to stimulating angiogenesis using 2 established models that distinctly represent inflammatory or ischemia\u2010driven angiogenesis, namely the periarterial femoral cuff and hind limb ischemia. AdM3 reduced intimal thickening, adventitial capillary density, and macrophage accumulation in femoral arteries 21 d after periarterial femoral cuff placement compared with AdGFP\u2010treated mice (\n                    <jats:italic>P<\/jats:italic>\n                    &lt; 0.05). AdM3 also reduced mRNA expression of proangiogenic VEGF, inflammatory markers IL\u20106 and IL\u20101\u03b2, and vascular smooth muscle cell (VSMC)\u2010activated synthetic markers Kr\u00fcppel\u2010like family of transcription factor 4 (KLF4) and platelet\u2010derived growth factor receptor \u03b2 (PDGFR\u03b2) in the inflammatory cuff model. In contrast, capillary density, VSMC content, blood flow perfusion, and VEGF gene expression were unaltered between groups in skeletal muscle following hind limb ischemia.\n                    <jats:italic>In vitro<\/jats:italic>\n                    , AdM3 significantly reduced human microvascular endothelial cell 1 proliferation, migration, and tubule formation by ~17, 71.3, and 8.7% (\n                    <jats:italic>P<\/jats:italic>\n                    &lt; 0.05) in macrophage\u2010conditioned medium associating with reduced VEGF and hypoxia\u2010inducible factor 1\u03b1 mRNA but not in hypoxia (1% O\n                    <jats:sub>2<\/jats:sub>\n                    ). Compared with AdGFP, AdM3 also inhibited VSMC proliferation and migration and reduced mRNA expression of KLF4 and PDGFR\u03b2 under inflammatory conditions. In contrast, AdM3 had no effect on VSMC processes in response to hypoxia\n                    <jats:italic>in vitro.<\/jats:italic>\n                    Our findings show that broad\u2010spectrum inhibition of inflammatory chemokines by M3 inhibits inflammatory\u2010driven but not ischemia\u2010driven angiogenesis, presenting a novel strategy for the treatment of diseases associated with inflammatory\u2010driven angiogenesis.\u2014Ravindran, D., Cartland, S. P., Bursill, C. A., Kavurma, M. M. Broad\u2010spectrum chemokine inhibition blocks inflammation\u2010induced angiogenesis, but preserves ischemia\u2010driven angiogenesis. FASEB J. 33, 13423\u201313434 (2019).\n                    <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/www.fasebj.org\">www.fasebj.org<\/jats:ext-link>\n                  <\/jats:p>","DOI":"10.1096\/fj.201900232rr","type":"journal-article","created":{"date-parts":[[2019,10,1]],"date-time":"2019-10-01T18:17:41Z","timestamp":1569953861000},"page":"13423-13434","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Broad\u2010spectrum chemokine inhibition blocks inflammation\u2010induced angiogenesis, but preserves ischemia\u2010driven angiogenesis"],"prefix":"10.1096","volume":"33","author":[{"given":"Dhanya","family":"Ravindran","sequence":"first","affiliation":[{"name":"The Heart Research Institute Sydney New South Wales Australia"},{"name":"Sydney Medical School The University of Sydney Sydney New South Wales Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Si\u00e2n P.","family":"Cartland","sequence":"additional","affiliation":[{"name":"The Heart Research Institute Sydney New South Wales Australia"},{"name":"Sydney Medical School The University of Sydney Sydney New South Wales Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christina A.","family":"Bursill","sequence":"additional","affiliation":[{"name":"Heart Health, South Australian Health and Medical Research Institute Adelaide South Australia Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mary M.","family":"Kavurma","sequence":"additional","affiliation":[{"name":"The Heart Research Institute Sydney New South Wales Australia"},{"name":"Sydney Medical School The University of Sydney Sydney New South Wales Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,10]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcmm.12164"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0008-6363(00)00281-9"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1097\/BCR.0b013e3181c7ed82"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm848"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0704966104"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1177\/1947601911423031"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jvs.2007.02.068"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.15283\/ijsc.2009.2.1.11"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1155\/2012\/918267"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1177\/1947601911423654"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.redox.2017.01.007"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjc.6603813"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.2302\/kjm.61.47"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0049-3848(12)70016-6"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/djq044"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1349-7006.2005.00059.x"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M408673200"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.177.4.2651"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.166.12.7571"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2003-05-1387"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.172.3.1681"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1521-4141(199907)29:07<2280::AID-IMMU2280>3.0.CO;2-C"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1093\/carcin\/bgu218"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.canlet.2015.01.033"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.2174\/138161210794455003"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm1075"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.191.3.573"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)63785-6"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.77.1.624-630.2003"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-80-1-75"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0173224"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(09)05209-4"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.177.10.7296"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.201600540R"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.109.206029"},{"key":"e_1_2_8_37_1","doi-asserted-by":"crossref","DOI":"10.1161\/JAHA.115.002527","article-title":"Tumor necrosis factor\u2010related apoptosis\u2010inducing ligand (TRAIL) promotes angiogenesis and ischemia\u2010induced neovascularization via NADPH oxidase 4 (NOX4) and nitric oxide\u2010dependent mechanisms","volume":"4","author":"DiBartolo B. A.","year":"2015","journal-title":"J. Am. Heart Assoc."},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1111\/1523-1747.ep12613748"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms17122025"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1012218401036"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0008-6363(98)00108-4"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.0000020561.03244.7E"},{"key":"e_1_2_8_43_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI118281"},{"key":"e_1_2_8_44_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.92.5.1230"},{"key":"e_1_2_8_45_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.80.4.1029"},{"key":"e_1_2_8_46_1","doi-asserted-by":"publisher","DOI":"10.1093\/cvr\/cvr323"},{"key":"e_1_2_8_47_1","first-page":"1667","article-title":"Mouse model of angiogenesis","volume":"152","author":"Couffinhal T.","year":"1998","journal-title":"Am. J. Pathol."},{"key":"e_1_2_8_48_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2009.185"},{"key":"e_1_2_8_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jvs.2012.04.067"},{"key":"e_1_2_8_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.atherosclerosis.2011.07.103"},{"key":"e_1_2_8_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vph.2006.08.008"},{"key":"e_1_2_8_52_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jacc.2013.09.019"},{"key":"e_1_2_8_53_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exphem.2006.04.002"},{"key":"e_1_2_8_54_1","doi-asserted-by":"publisher","DOI":"10.1161\/ATVBAHA.107.139642"},{"key":"e_1_2_8_55_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00011-004-1299-4"},{"key":"e_1_2_8_56_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.0000163017.13772.3a"},{"key":"e_1_2_8_57_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jvs.2004.07.012"},{"key":"e_1_2_8_58_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.ATV.20.2.335"},{"key":"e_1_2_8_59_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.84.3.306"},{"key":"e_1_2_8_60_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.0000028590.02477.6F"},{"key":"e_1_2_8_61_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1054-8807(02)00111-4"},{"key":"e_1_2_8_62_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.0000097119.57756.EF"},{"key":"e_1_2_8_63_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)80438-9"},{"key":"e_1_2_8_64_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.ATV.0000234947.47788.8c"},{"key":"e_1_2_8_65_1","doi-asserted-by":"publisher","DOI":"10.1161\/ATVBAHA.115.305789"},{"key":"e_1_2_8_66_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0113752"},{"key":"e_1_2_8_67_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.115.306361"},{"key":"e_1_2_8_68_1","doi-asserted-by":"publisher","DOI":"10.1159\/000490024"},{"key":"e_1_2_8_69_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9150(99)00033-7"}],"container-title":["The FASEB Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1096\/fj.201900232RR","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1096\/fj.201900232RR","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/faseb.onlinelibrary.wiley.com\/doi\/pdf\/10.1096\/fj.201900232RR","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T21:48:40Z","timestamp":1761688120000},"score":1,"resource":{"primary":{"URL":"https:\/\/faseb.onlinelibrary.wiley.com\/doi\/10.1096\/fj.201900232RR"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,10]]},"references-count":68,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2019,12]]}},"alternative-id":["10.1096\/fj.201900232RR"],"URL":"https:\/\/doi.org\/10.1096\/fj.201900232rr","archive":["Portico"],"relation":{},"ISSN":["0892-6638","1530-6860"],"issn-type":[{"value":"0892-6638","type":"print"},{"value":"1530-6860","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,10]]},"assertion":[{"value":"2019-01-27","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-08-26","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-10-01","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}