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We have previously shown that bone loss can be prevented by mechanical loading, but the efficacy of ankle loading for metastasis\u2010linked bone loss has not been investigated. This study showed that body weight was decreased after inoculation of tumor cells, but ankle loading restored a rapid weight loss. The nonloading group exhibited a decrease in bone volume\/tissue volume (BV\/TV), trabecular thickness, and trabecular number (all\n                    <jats:italic>P<\/jats:italic>\n                    &lt; 0.01) as well as an increase in trabecular separation (\n                    <jats:italic>P<\/jats:italic>\n                    &lt; 0.001). However, ankle loading improved those changes (all\n                    <jats:italic>P<\/jats:italic>\n                    &lt; 0.05). Furthermore, although the nonloading group increased the tumor bearing as well as expression of IL\u20108 and matrix metalloproteinase 9, ankle loading decreased them. Induction of tumor in the bone elevated the osteoclast number (\n                    <jats:italic>P<\/jats:italic>\n                    &lt; 0.05) as well as the levels of nuclear factor of activated T\u2010cells cytoplasmic 1, NF\u2010\u03baB ligand, cathepsin K, and serum tartrate\u2010resistant acid phosphatase type 5b, but ankle loading reduced osteoclast activity and those levels (all\n                    <jats:italic>P<\/jats:italic>\n                    &lt; 0.05). Tumor bearing was positively correlated with the osteoclast number (\n                    <jats:italic>P<\/jats:italic>\n                    &lt; 0.01) and negatively correlated with BV\/TV and the osteoblast number (both\n                    <jats:italic>P<\/jats:italic>\n                    &lt; 0.01). Collectively, these findings demonstrate that ankle loading suppresses tumor growth and osteolysis by inhibiting bone resorption and enhancing bone formation.\u2014Yang, S., Liu, H., Zhu, L., Li, X., Liu, D., Song, X., Yokota, H., Zhang, P. Ankle loading ameliorates bone loss from breast cancer\u2013associated bone metastasis. FASEB J. 33, 10742\u201310752 (2019).\n                    <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/www.fasebj.org\">www.fasebj.org<\/jats:ext-link>\n                  <\/jats:p>","DOI":"10.1096\/fj.201900306rr","type":"journal-article","created":{"date-parts":[[2019,7,2]],"date-time":"2019-07-02T20:50:55Z","timestamp":1562100655000},"page":"10742-10752","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Ankle loading ameliorates bone loss from breast cancer\u2013associated bone metastasis"],"prefix":"10.1096","volume":"33","author":[{"given":"Shuang","family":"Yang","sequence":"first","affiliation":[{"name":"Department of Anatomy and Histology School of Basic Medical Sciences Tianjin Medical University Tianjin China"},{"name":"Key Laboratory of Hormones and Development, Ministry of Health Tianjin Key Laboratory of Metabolic Diseases Tianjin Medical University Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong","family":"Liu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Cancer Prevention and Therapy, Ministry of Education Tianjin Medical University Tianjin China"},{"name":"Department of Breast Surgery Tianjin Medical University Cancer Institute and Hospital Tianjin China"},{"name":"Key Laboratory of Cancer Prevention and Therapy Tianjin China"},{"name":"Tianjin's Clinical Research Center for Cancer Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lei","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Molecular Imaging and Nuclear Medicine National Clinical Research Center for Cancer Tianjin Medical University Cancer Institute and Hospital Tianjin China"},{"name":"Key Laboratory of Cancer Prevention and Therapy Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinle","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Histology School of Basic Medical Sciences Tianjin Medical University Tianjin China"},{"name":"Key Laboratory of Hormones and Development, Ministry of Health Tianjin Key Laboratory of Metabolic Diseases Tianjin Medical University Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daquan","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Histology School of Basic Medical Sciences Tianjin Medical University Tianjin China"},{"name":"Key Laboratory of Hormones and Development, Ministry of Health Tianjin Key Laboratory of Metabolic Diseases Tianjin Medical University Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaomeng","family":"Song","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Histology School of Basic Medical Sciences Tianjin Medical University Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroki","family":"Yokota","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering Indiana University-Purdue University Indianapolis Indianapolis Indiana USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ping","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Histology School of Basic Medical Sciences Tianjin Medical University Tianjin China"},{"name":"Key Laboratory of Hormones and Development, Ministry of Health Tianjin Key Laboratory of Metabolic Diseases Tianjin Medical University Tianjin China"},{"name":"Tianjin Key Laboratory of Spine and Spinal Cord Tianjin Medical University Tianjin China"},{"name":"Tianjin's Clinical Research Center for Cancer Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,7,2]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21218"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.canlet.2014.04.007"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-06-0931"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbo.2013.09.001"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbo.2018.01.004"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.breast.2015.07.018"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.7314\/APJCP.2014.15.4.1503"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.breast.2017.10.007"},{"key":"e_1_2_8_10_1","first-page":"CD008455","article-title":"Interventions for treating bisphosphonate-related osteonecrosis of the jaw (BRONJ)","volume":"2","author":"Rollason V.","year":"2016","journal-title":"Cochrane Database Syst. 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