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We aimed to characterize the EX sampled from human hamstring or calf muscles following a strain injury (\n                    <jats:italic>n<\/jats:italic>\n                    = 12). The cytokine and growth\u2010factor profile, gene expression, and transcriptome analysis of EX\u2010derived cells were compared with blood taken simultaneously from the same individuals. Cellular responses to the EX were tested in 3\u2010dimensional (3D) culture based on primary human fibroblasts and myoblasts isolated from hamstring muscles. The EX contained a highly proinflammatory profile with a substantial expression of angiogenic factors. The proinflammatory profile was present in samples taken early postinjury and in samples aspirated several weeks postinjury, suggesting persistent inflammation. Cells derived from the EX demonstrated an increased expression of fibrogenic, adipogenic, and angiogenesis\u2010related genes in comparison with blood cells. The injury EX stimulated fibroblast proliferation 2\u2010fold compared with plasma, whereas such an effect was not seen for myoblasts. Finally, in 3D cell culture, the EX induced an up\u2010regulation of connective tissue\u2010related genes. In summary, EX formation following a muscle\u2010strain injury stimulates fibroblast proliferation and the synthesis of connective tissue in fibroblasts. This suggests that the EX promotes an acute tissue\u2010healing response but potentially also contributes to the formation of fibrotic tissue in the later phases of tissue repair.\u2014Bayer, M. L., Bang, L., Hoegberget\u2010Kalisz, M., Svensson, R. B., Olesen, J. L., Karlsson, M. M., Schjerling, P., Hellsten, Y., Hoier, B., Magnusson, S. P., Kjaer, M. Muscle\u2010strain injury exudate favors acute tissue healing and prolonged connective tissue formation in humans. FASEB J. 33,10369\u201310382 (2019).\n                    <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/www.fasebj.org\">www.fasebj.org<\/jats:ext-link>\n                  <\/jats:p>","DOI":"10.1096\/fj.201900542r","type":"journal-article","created":{"date-parts":[[2019,6,18]],"date-time":"2019-06-18T19:52:39Z","timestamp":1560887559000},"page":"10369-10382","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Muscle\u2010strain injury exudate favors acute tissue healing and prolonged connective tissue formation in humans"],"prefix":"10.1096","volume":"33","author":[{"given":"Monika L.","family":"Bayer","sequence":"first","affiliation":[{"name":"Department of Orthopedic Surgery M Institute of Sports Medicine Copenhagen Bispebjerg Hospital  Copenhagen Denmark"},{"name":"Center for Healthy Aging Faculty of Health and Medical Sciences University of Copenhagen  Copenhagen 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