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Here, we examined the ovarian functions of the Hippo kinases large tumor suppressors (LATS)1 and 2, which serve to inactivate the transcriptional coactivators Yes\u2010associated protein (YAP) and transcriptional coactivator with PDZ\u2010binding motif (TAZ). Inactivation of\n                    <jats:italic>Lats1\/2<\/jats:italic>\n                    in murine granulosa cells either\n                    <jats:italic>in vitro<\/jats:italic>\n                    or\n                    <jats:italic>in vivo<\/jats:italic>\n                    resulted in a loss of granulosa cell morphology, function, and gene expression. Mutant cells further underwent changes in structure and gene expression suggestive of epithelial\u2010to\u2010mesenchymal transition and transdifferentiation into multiple lineages.\n                    <jats:italic>In vivo<\/jats:italic>\n                    , granulosa cell\u2010specific loss of\n                    <jats:italic>Lats1\/2<\/jats:italic>\n                    caused the ovarian parenchyma to be mostly replaced by bone tissue and seminiferous tubule\u2010like structures. Transdifferentiation into Sertoli\u2010like cells and osteoblasts was attributed in part to the increased recruitment of YAP and TAZ to the promoters of sex\u2010determining region Y box 9 and bone \u03b3\u2010carboxyglutamate protein, key mediators of male sex determination and osteogenesis, respectively. Together, these results demonstrate for the first time a critical role for\n                    <jats:italic>Lats1\/2<\/jats:italic>\n                    in the maintenance of the granulosa cell genetic program and further highlight the remarkable plasticity of granulosa cells.\u2014Tsoi, M., Morin, M., Rico, C., Johnson, R. L., Paquet, M., G\u00e9vry, N., Boerboom, D.\n                    <jats:italic>Lats1<\/jats:italic>\n                    and\n                    <jats:italic>L\u03b1ts2<\/jats:italic>\n                    are required for ovarian granulosa cell fate maintenance. FASEB J. 33, 10819\u201310832 (2019).\n                    <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/www.fasebj.org\">www.fasebj.org<\/jats:ext-link>\n                  <\/jats:p>","DOI":"10.1096\/fj.201900609r","type":"journal-article","created":{"date-parts":[[2019,7,3]],"date-time":"2019-07-03T16:27:27Z","timestamp":1562171247000},"page":"10819-10832","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":45,"title":["<i>Lats1<\/i>\n                    and\n                    <i>Lats2<\/i>\n                    are required for ovarian granulosa cell fate maintenance"],"prefix":"10.1096","volume":"33","author":[{"given":"Mayra","family":"Tsoi","sequence":"first","affiliation":[{"name":"D\u00e9partement de Biom\u00e9decine V\u00e9t\u00e9rinaire Centre de Recherche en Reproduction et Fertilit\u00e9, Facult\u00e9 de M\u00e9decine V\u00e9t\u00e9rinaire Universit\u00e9 de Montr\u00e9al Saint-Hyacinthe Qu\u00e9bec Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin","family":"Morin","sequence":"additional","affiliation":[{"name":"D\u00e9partement de Biologie Facult\u00e9 des Sciences Universit\u00e9 de Sherbrooke Sherbrooke Qu\u00e9bec Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charl\u00e8ne","family":"Rico","sequence":"additional","affiliation":[{"name":"D\u00e9partement de Biom\u00e9decine V\u00e9t\u00e9rinaire Centre de Recherche en Reproduction et Fertilit\u00e9, Facult\u00e9 de M\u00e9decine V\u00e9t\u00e9rinaire Universit\u00e9 de Montr\u00e9al Saint-Hyacinthe Qu\u00e9bec Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Randy L.","family":"Johnson","sequence":"additional","affiliation":[{"name":"Department of Cancer Biology The University of Texas M. 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Anderson Cancer Center Houston Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maril\u00e8ne","family":"Paquet","sequence":"additional","affiliation":[{"name":"D\u00e9partement de Pathologie et de Microbiologie Centre de Recherche en Reproduction et Fertilit\u00e9 Facult\u00e9 de M\u00e9decine V\u00e9t\u00e9rinaire Universit\u00e9 de Montr\u00e9al Saint-Hyacinthe Qu\u00e9bec Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicolas","family":"G\u00e9vry","sequence":"additional","affiliation":[{"name":"D\u00e9partement de Biologie Facult\u00e9 des Sciences Universit\u00e9 de Sherbrooke Sherbrooke Qu\u00e9bec Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Derek","family":"Boerboom","sequence":"additional","affiliation":[{"name":"D\u00e9partement de Biom\u00e9decine V\u00e9t\u00e9rinaire Centre de Recherche en Reproduction et Fertilit\u00e9, Facult\u00e9 de M\u00e9decine V\u00e9t\u00e9rinaire Universit\u00e9 de Montr\u00e9al Saint-Hyacinthe Qu\u00e9bec Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,7,3]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.1909210"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2010.09.011"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00005.2014"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature10137"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.1602907"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1742-4658.2006.05427.x"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2008.02.006"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1208445"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm2822"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M110.152942"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.1843810"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1097-2765(02)00776-1"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2007.07.019"},{"key":"e_1_2_9_15_1","doi-asserted-by":"crossref","first-page":"1229","DOI":"10.1101\/gad.888601","article-title":"TEAD\/TEF transcription factors utilize the activation domain of YAP65, a Src\/Yes-associated protein localized in the cytoplasm","volume":"15","author":"Vassilev A.","year":"2001","journal-title":"GenesDev."},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.1664408"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.4161\/cbt.6.6.4241"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/dgd.12335"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2009.02.003"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mod.2007.11.002"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ceb.2017.12.012"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms11961"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dld.2015.05.011"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.119008"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.2016060611"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2014.03.060"},{"key":"e_1_2_9_27_1","doi-asserted-by":"crossref","first-page":"24804","DOI":"10.18632\/oncotarget.15063","article-title":"S100A7 promotes lung adenocarcinoma to squamous carcinoma transdifferentiation, and its expression is differentially regulated by the Hippo-YAP pathway in lung cancer cells","volume":"8","author":"Wang R.","year":"2017","journal-title":"Oncotarget"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/5965"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1312830110"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.192856.112"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.14-267856"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1199010"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/29.9.e45"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2008.211"},{"key":"e_1_2_9_35_1","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1007\/978-1-60327-015-1_16","article-title":"Chromatin immunoprecipitation in mammalian cells","volume":"543","author":"Svotelis A.","year":"2009","journal-title":"Methods Mol. 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