{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T09:11:31Z","timestamp":1790241091558,"version":"4.1.0"},"reference-count":56,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2019,8,23]],"date-time":"2019-08-23T00:00:00Z","timestamp":1566518400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100000925","name":"National Health and Medical Research Council","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000925","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2019,11]]},"abstract":"<jats:p>\n                    GNA2091 is one of the components of the 4\u2010component meningococcal serogroup B vaccine (4CMenB) vaccine and is highly conserved in all meningococcal strains. However, its functional role has not been fully characterized. Here we show that\n                    <jats:italic>nmb2091<\/jats:italic>\n                    is part of an operon and is cotranscribed with the\n                    <jats:italic>nmb2089, nmb2090<\/jats:italic>\n                    , and\n                    <jats:italic>nmb2092<\/jats:italic>\n                    adjacent genes, and a similar but reduced operon arrangement is conserved in many other gram\u2010negative bacteria. Deletion of the\n                    <jats:italic>nmb2091<\/jats:italic>\n                    gene causes an aggregative phenotype with a mild defect in cell separation; differences in the outer membrane composition and phospholipid profile, in particular in the phosphoethanolamine levels; an increased level of outer membrane vesicles; and deregulation of the zinc\u2010responsive genes such as\n                    <jats:italic>znuD.<\/jats:italic>\n                    Finally, the \u03942091 strain is attenuated with respect to the wild\u2010type strain in competitive index experiments in the infant rat model of meningococcal infection. Altogether these data suggest that GNA2091 plays important roles in outer membrane architecture, biogenesis, homeostasis, and in meningococcal survival\n                    <jats:italic>in vivo, and<\/jats:italic>\n                    amodel for its role is discussed. These findings highlight the importance of GNA2091 as a vaccine component.\u2014Seib, K. L., Haag, A. F., Oriente, F., Fantappi\u00e8, L., Borghi, S., Semchenko, E. A., Schulz, B. L., Ferlicca, F., Taddei, A. R., Giuliani, M. M., Pizza, M., Delany, I. The meningococcal vaccine antigen GNA2091 is an analogue of YraP and plays key roles in outer membrane stability and virulence. FASEB J. 33, 12324\u201012335 (2019).\n                    <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/www.fasebj.org\">www.fasebj.org<\/jats:ext-link>\n                  <\/jats:p>","DOI":"10.1096\/fj.201900669r","type":"journal-article","created":{"date-parts":[[2019,8,23]],"date-time":"2019-08-23T15:37:33Z","timestamp":1566574653000},"page":"12324-12335","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["The meningococcal vaccine antigen GNA2091 is an analogue of YraP and plays key roles in outer membrane stability and virulence"],"prefix":"10.1096","volume":"33","author":[{"given":"Kate L.","family":"Seib","sequence":"first","affiliation":[{"name":"Institute for Glycomics Griffith University Gold Coast Queensland Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andreas 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