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An emerging therapeutic strategy for osteoarthritis (OA) is to selectively eliminate senescent cells by initiating apoptosis. This study establishes a cartilage explant model of senescence induction and senolytic clearance using\n                    <jats:italic>\n                      p16\n                      <jats:sup>Ink4a<\/jats:sup>\n                    <\/jats:italic>\n                    expression as a biomarker of senescence. Growth\u2010factor stimulation of explants increased the expression of p16\n                    <jats:sup>Ink4a<\/jats:sup>\n                    at both the mRNA and protein levels. Applying this culture system to cartilage from p16tdTom reporter mice (a knockin allele with tdTomato fluorescent protein regulated by the endogenous p16Ink4a promoter) demonstrated the emergence of a p16\u2010high population that was quantified using flow cytometry for tdTomato. Cell sorting was used to separate chondrocytes based on tdTomato fluorescence and p16\u2010high cells showed higher senescence\u2010associated \u03b2\u2010galactosidase activity and increased gene expression of the senescence\u2010associated secretory phenotype as compared with p16\u2010low cells. The potential for effective senolysis within the cartilage extracellular matrix was assessed using navitoclax (ABT\u2010263). Navitoclax treatment reduced the percentage of p16\u2010high cells from 17.9 to 6.1% (mean of 13 matched pairs;\n                    <jats:italic>P<\/jats:italic>\n                    &lt; 0.001) and increased cleaved caspase\u20103 confirmed apoptotic activity. Together, these findings establish a physiologically relevant cartilage explant model for testing the induction and elimination of senescent chondrocytes, which will support investigations of senolytic therapy for OA.\u2014Sessions, G. A., Copp, M. E., Liu, J.\u2010Y., Sinkler, M. A., D'Costa, S., Diekman, B. O. Controlled induction and targeted elimination of p16\n                    <jats:sup>INK4a<\/jats:sup>\n                    \u2010expressing chondrocytes in cartilage explant culture. FASEB J. 33, 12364\u201312373 (2019).\n                    <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/www.fasebj.org\">www.fasebj.org<\/jats:ext-link>\n                  <\/jats:p>","DOI":"10.1096\/fj.201900815rr","type":"journal-article","created":{"date-parts":[[2019,8,13]],"date-time":"2019-08-13T14:12:36Z","timestamp":1565705556000},"page":"12364-12373","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":43,"title":["Controlled induction and targeted elimination of p16\n                    <sup>INK4a<\/sup>\n                    \u2010expressing chondrocytes in cartilage explant culture"],"prefix":"10.1096","volume":"33","author":[{"given":"Garrett A.","family":"Sessions","sequence":"first","affiliation":[{"name":"Thurston Arthritis Research Center"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michaela 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