{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,21]],"date-time":"2026-04-21T17:00:16Z","timestamp":1776790816295,"version":"3.51.2"},"reference-count":31,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2019,10,31]],"date-time":"2019-10-31T00:00:00Z","timestamp":1572480000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003715","name":"Korea Research Institute of Bioscience and Biotechnology","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003715","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["NRF\u20102017R1D1A1B03031420"],"award-info":[{"award-number":["NRF\u20102017R1D1A1B03031420"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2019,12]]},"abstract":"<jats:p>\n                    The glycosylphosphatidylinositol\u2010anchored sperm hyaluronidases (Hyals), sperm adhesion molecule 1 (SPAM1) and HYAL5, have long been believed to assist in sperm penetration through the cumulus\u2010oocyte complex (COC), but their role in mammalian fertilization remains unclear. Previously, we have shown that mouse sperm devoid of either\n                    <jats:italic>Spam1<\/jats:italic>\n                    or\n                    <jats:italic>Hyal5<\/jats:italic>\n                    are still capable of penetrating the COC and that the loss of either\n                    <jats:italic>Spam1<\/jats:italic>\n                    or\n                    <jats:italic>Hyal5<\/jats:italic>\n                    alone does not cause male infertility in mice. In the present study, we found that\n                    <jats:italic>Spam1\/Hyal5<\/jats:italic>\n                    double knockout (dKO) mice produced significantly fewer offspring compared with wild\u2010type (WT) mice, and this was due to defective COC dispersal. A comparative analysis between WT and\n                    <jats:italic>Spam1\/Hyal5<\/jats:italic>\n                    dKO epididymal sperm revealed that the absence of these 2 sperm Hyals resulted in a marked accumulation of sperm on the outside of the COC. This impaired sperm activity is likely due to the deficiency in the sperm Hyals, even though other somatic Hyals are expressed normally in the dKO mice. The fertilization ability of the\n                    <jats:italic>Spam1\/Hyal5<\/jats:italic>\n                    dKO sperm was restored by adding purified human sperm Hyal to the\n                    <jats:italic>in vitro<\/jats:italic>\n                    fertilization medium. Our results suggest that Hyal deficiency in sperm may be a significant risk factor for male sterility.\u2014Park, S., Kim, Y.\u2010H., Jeong, P.\u2010S., Park, C., Lee, J.\u2010W., Kim, J.\u2010S., Wee, G., Song, B.\u2010S., Park, B.\u2010J., Kim, S.\u2010H., Sim, B.\u2010W., Kim, S.\u2010U., Triggs\u2010Raine, B., Baba, T., Lee, S.\u2010R., Kim, E. SPAM1\/HYAL5 double deficiency in male mice leads to severe male subfertility caused by a cumulus\u2010oocyte complex penetration defect. FASEB J. 33, 14440\u201014449 (2019).\n                    <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/www.fasebj.org\">www.fasebj.org<\/jats:ext-link>\n                  <\/jats:p>","DOI":"10.1096\/fj.201900889rrr","type":"journal-article","created":{"date-parts":[[2019,10,31]],"date-time":"2019-10-31T14:49:25Z","timestamp":1572533365000},"page":"14440-14449","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["SPAM1\/HYAL5 double deficiency in male mice leads to severe male subfertility caused by a cumulus\u2010oocyte complex penetration defect"],"prefix":"10.1096","volume":"33","author":[{"given":"Soojin","family":"Park","sequence":"first","affiliation":[{"name":"College of Pharmacy Catholic University of Daegu Gyeongsan\u2010si South 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of Bioscience and Biotechnology (KRIBB) Deajeon South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ju\u2010Sung","family":"Kim","sequence":"additional","affiliation":[{"name":"College of Applied Life Sciences, Research Institute for Subtropical Agriculture and Biotechnology Jeju National University Jeju South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gabin","family":"Wee","sequence":"additional","affiliation":[{"name":"Laboratory Animal Center Daegu\u2010Gyeongbuk Medical Innovation Foundation Daegu South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bong\u2010Seok","family":"Song","sequence":"additional","affiliation":[{"name":"Futuristic Animal Resource and Research Center Korea Research Institute of Bioscience and Biotechnology Chungcheongbuk\u2010do South 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