{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T20:01:31Z","timestamp":1790625691869,"version":"4.1.0"},"reference-count":30,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2020,7,6]],"date-time":"2020-07-06T00:00:00Z","timestamp":1593993600000},"content-version":"am","delay-in-days":366,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#am"},{"start":{"date-parts":[[2019,7,6]],"date-time":"2019-07-06T00:00:00Z","timestamp":1562371200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000163","name":"Division of Arctic Sciences","doi-asserted-by":"publisher","award":["DP160100959"],"award-info":[{"award-number":["DP160100959"]}],"id":[{"id":"10.13039\/100000163","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2019,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Antimicrobial peptides (AMPs) that target lipid membranes show promise as alternatives to conventional antibiotics. However, the molecular mechanisms of membrane perturbation, as most studies are performed in model systems and in\u2010cell structural studies, have yet to be achieved. Solid\u2010state NMR spectroscopy is a valuable technique to investigate peptide\u2010membrane interactions and to determine the structure of peptides, but the short lifespan of bacteria, especially under magic angle spinning conditions, has not permitted in\u2010cell structural studies. Here, we present the first dynamic nuclear polarization (DNP)\u2010NMR in\u2010cell studies of\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    bacteria incubated with the AMP maculatin 1.1 (Mac1) in combination with novel nitroxide spin\u2010labeled peptides 2,2,6,6\u2010tetramethylpi\u2010peridine\u2010N\u2010oxyl\u20104\u2010amino\u20104\u2010carboxylic acid (TOAC)\u2013[F3W]\u2010Mac1 (MacW) and TOAC\u2010TOAC\u2010MacW. The in\u2010cell\n                    <jats:sup>13<\/jats:sup>\n                    C and\n                    <jats:sup>15<\/jats:sup>\n                    N signal NMR enhancements, and\n                    <jats:sup>1<\/jats:sup>\n                    H spin\u2010lattice\n                    <jats:italic>T<\/jats:italic>\n                    <jats:sub>1<\/jats:sub>\n                    relaxation times showed that TOAC\u2010MacW and TOAC\u2010TOAC\u2010MacW performed better than the more hydrophilic biradical AMUPol used for DNP studies. Furthermore, the pores formed by the AMP increased the signal enhancements and decreased\n                    <jats:italic>T<\/jats:italic>\n                    <jats:sub>1<\/jats:sub>\n                    values of specifically\n                    <jats:sup>13<\/jats:sup>\n                    C\u2010 and\n                    <jats:sup>15<\/jats:sup>\n                    N\u2010labeled Mac1. This approach has a great potential for determining the first\n                    <jats:italic>in situ<\/jats:italic>\n                    structures of AMPs in bacteria.\u2014Sani, M.\u2010A., Zhu, S., Hofferek, V., Separovic, F. Nitroxide spin\u2013labeled peptides for DNP\u2010NMR in\u2010cell studies. FASEB J. 33, 11021\u201311027 (2019).\n                    <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/www.fasebj.org\">www.fasebj.org<\/jats:ext-link>\n                  <\/jats:p>","DOI":"10.1096\/fj.201900931r","type":"journal-article","created":{"date-parts":[[2019,7,8]],"date-time":"2019-07-08T21:31:56Z","timestamp":1562621516000},"page":"11021-11027","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":36,"title":["Nitroxide spin\u2013labeled peptides for DNP\u2010NMR in\u2010cell studies"],"prefix":"10.1096","volume":"33","author":[{"given":"Marc-Antoine","family":"Sani","sequence":"first","affiliation":[{"name":"School of Chemistry Bio21 Institute University of Melbourne Melbourne Victoria Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shiying","family":"Zhu","sequence":"additional","affiliation":[{"name":"School of Chemistry Bio21 Institute University of Melbourne Melbourne Victoria Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vinzenz","family":"Hofferek","sequence":"additional","affiliation":[{"name":"School of Chemistry Bio21 Institute University of Melbourne Melbourne Victoria Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frances","family":"Separovic","sequence":"additional","affiliation":[{"name":"School of Chemistry Bio21 Institute University of Melbourne Melbourne Victoria Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,7,6]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.accounts.6b00074"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1021\/cb1001558"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.3389\/fchem.2019.00043"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1529\/biophysj.105.073890"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1039\/9781782627449-00287"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-62703-583-5_9"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms20010181"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1021\/ar300348n"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja405813t"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201410249"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamem.2014.07.008"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/cphc.201100630"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/chem.201404588"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/chem.201501376"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/pro.600"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2013.08.035"},{"key":"e_1_2_8_18_1","first-page":"173","article-title":"pH dependence of EPR spectra of nitroxides containing ionizable groups","volume":"14","author":"Nakaie C. 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