{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T23:39:30Z","timestamp":1761694770175,"version":"build-2065373602"},"reference-count":38,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2019,9,18]],"date-time":"2019-09-18T00:00:00Z","timestamp":1568764800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/2.0\/"}],"funder":[{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["2016R1A2B4007904","2019M3A9A8065054","2019R1C1C1005027"],"award-info":[{"award-number":["2016R1A2B4007904","2019M3A9A8065054","2019R1C1C1005027"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2019,11]]},"abstract":"<jats:p>\n                    Protein tyrosine kinase 7 (PTK7), a catalytically defective receptor protein tyrosine kinase (RPTK), plays an oncogenic role by activating an unidentified TKI\u2010258 (dovitinib)\u2010sensitive RPTK in esophageal squamous cell carcinoma (ESCC) cells. Here, we demonstrate that among TKI\u2010258\u2010sensitive RPTKs, fibroblast growth factor receptor (FGFR) 1 is significantly up\u2010regulated in ESCC tissues and cell lines. We show that PTK7 colocalizes with FGFR1 and binds it\n                    <jats:italic>via<\/jats:italic>\n                    its extracellular domain in human embryonic kidney 293 and ESCC TE\u201010 cells. PTK7 knockdown not only reduced ligand\u2010free and fibroblast growth factor (FGF)\u2010induced phosphorylation of FGFR1 but also the interaction of signaling adaptor proteins with FGFR1 and activation of downstream signaling proteins in TE\u201010 cells. In addition, PTK7 knockdown reduced FGF\u2010induced oncogenic phenotypes including proliferation, anchorage\u2010independent colony formation, wound healing, and invasion in ESCC cells. Taken together, our data demonstrate that PTK7 binds and activates FGFR1 independent of FGF and thus increases oncogenicity of PTK7\u2010 and FGFR1\u2010positive cancers such as ESCC.\u2014Shin, W.\u2010S., Lee, H. W., Lee, S.\u2010T. Catalytically inactive receptor tyrosine kinase PTK7 activates FGFR1 independent of FGF. FASEB J. 33, 12960\u201312971 (2019).\n                    <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/www.fasebj.org\">www.fasebj.org<\/jats:ext-link>\n                  <\/jats:p>","DOI":"10.1096\/fj.201900932r","type":"journal-article","created":{"date-parts":[[2019,9,6]],"date-time":"2019-09-06T14:11:04Z","timestamp":1567779064000},"page":"12960-12971","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Catalytically inactive receptor tyrosine kinase PTK7 activates FGFR1 independent of FGF"],"prefix":"10.1096","volume":"33","author":[{"given":"Won\u2010Sik","family":"Shin","sequence":"first","affiliation":[{"name":"Department of Biochemistry College of Life Science and Biotechnology, Yonsei University Seoul Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hae Won","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Thoracic Surgery Korea Cancer Center Hospital, Korea Institute of Radiological and Medical Sciences Seoul Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Seung\u2010Taek","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Biochemistry College of Life Science and Biotechnology, Yonsei University Seoul Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,9,18]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1042\/BST20160331"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccr.2014.02.025"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.canlet.2018.07.030"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M112.414441"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/celldisc.2017.15"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/cas.12194"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12943-018-0790-4"},{"key":"e_1_2_8_9_1","first-page":"2179","article-title":"Colon carcinoma kinase\u20104 defines a new subclass of the receptor tyrosine kinase family","volume":"11","author":"Mossie K.","year":"1995","journal-title":"Oncogene"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1065817"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-0954-03"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/gcc.20136"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-10-3588"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0090247"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0014018"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2010-01-262352"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.12303"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12957-017-1172-x"},{"key":"e_1_2_8_19_1","first-page":"7881","article-title":"PTK7 protein is decreased in epithelial ovarian carcinomas with poor prognosis","volume":"7","author":"Wang H.","year":"2014","journal-title":"Int. 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