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Sertoli cells are the major targets of MuV infection. However, the mechanisms by which MuV infection impairs male fertility and Sertoli cell function remain unclear. The present study elucidated the effect of MuV infection on the blood\u2010testis barrier (BTB). The transepithelial electrical resistance of MuV\u2010infected mouse Sertoli cells was monitored, and the expression of major proteins of the BTB was examined. We demonstrated that MuV infection disrupted the BTB by reducing the levels of occludin and zonula occludens 1. Sertoli cells derived from\n                    <jats:italic>Tlr2<\/jats:italic>\n                    <jats:sup>\u2013\/\u2013<\/jats:sup>\n                    and\n                    <jats:italic>Tnfa<\/jats:italic>\n                    <jats:sup>\u2013\/\u2013<\/jats:sup>\n                    mice were analyzed for mediating MuV\u2010induced impairment. TLR2\u2010mediated TNF\u2010\u03b1 production by Sertoli cells in response to MuV infection impaired BTB integrity. MuV\u2010impaired BTB was not observed in\n                    <jats:italic>Tlr2<\/jats:italic>\n                    <jats:sup>\u2013\/\u2013<\/jats:sup>\n                    and\n                    <jats:italic>Tnfa<\/jats:italic>\n                    <jats:sup>\u2013\/\u2013<\/jats:sup>\n                    Sertoli cells. Moreover, an inhibitor of TNF\u2010\u03b1, pomalidomide, prevents the disruption of BTB in response to MuV infection. FITC\u2010labeled biotin tracing assay confirmed that BTB permeability and spermatogenesis were transiently impaired by MuV infection\n                    <jats:italic>in vivo<\/jats:italic>\n                    . These findings suggest that the disruption of the BTB could be one of the mechanisms underlying MuV\u2010impaired male fertility, in which TNF\u2010\u03b1 could play a critical role.\u2014Wu, H., Jiang, X., Gao, Y., Liu, W., Wang, F., Gong, M., Chen, R., Yu, X., Zhang, W., Gao, B., Song, C., Han, D. Mumps virus infection disrupts blood\u2010testis barrier through the induction of TNF\u2010\u03b1 in Sertoli cells. FASEB J. 33, 12528\u201312540 (2019).\n                    <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/www.fasebj.org\">www.fasebj.org<\/jats:ext-link>\n                  <\/jats:p>","DOI":"10.1096\/fj.201901089r","type":"journal-article","created":{"date-parts":[[2019,8,26]],"date-time":"2019-08-26T22:36:32Z","timestamp":1566858992000},"page":"12528-12540","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":84,"title":["Mumps virus infection disrupts blood\u2010testis barrier through the induction of TNF\u2010\u03b1 in Sertoli cells"],"prefix":"10.1096","volume":"33","author":[{"given":"Han","family":"Wu","sequence":"first","affiliation":[{"name":"College of Animal Science and Technology, Institute of Mobilome and Genome, Yangzhou University Yangzhou China"},{"name":"Institute of Basic Medical Sciences Peking Union Medical College\u2013Chinese Academy of Medical Sciences Beijing 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