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Low bone mineral density is an independent and significant prognostic factor for curve progression. Currently, the cause underlying low bone mass in AIS remains elusive. Osteocytes play an important role in bone metabolism and mineral homeostasis, but its role in AIS has not been studied. In the present study, iliac bone tissues were harvested from 21 patients with AIS (mean age of 14.3 \u00b1 2.20 yr old) with a mean Cobb angle of 55.6 \u00b1 10.61\u00b0 and 13 non\u2010AIS controls (mean age of 16.5 \u00b1 4.79 yr old) intraoperatively. Acid\u2010etched scanning electron microscopy (SEM) images of AIS demonstrated abnormal osteocytes that were more rounded and cobblestone\u2010like in shape and were aligned in irregular clusters with shorter and disorganized canaliculi. Further quantitative analysis with FITC\u2010Imaris technique showed a significant reduction in the canalicular number and length as well as an increase in lacunar volume and area in AIS. SEM with energy\u2010dispersive X\u2010ray spectroscopy analysis demonstrated a lower calcium\u2010to\u2010phosphorus ratio at the perilacunar\/canalicular region. Moreover, microindentaion results revealed lower values of Vickers hardness and elastic modulus in AIS when compared with controls. In addition, in the parallel study of 99 AIS (27 with severe Cobb angle of 65.8 \u00b1 14.1\u00b0 and 72 with mild Cobb angle of 26.6 \u00b1 9.1\u00b0) with different curve severity, the serum osteocalcin level was found to be significantly and negatively associated with the Cobb angle. In summary, the findings in this series of studies demonstrated the potential link of abnormal osteocyte lacuno\u2010canalicular network structure and function to the observed abnormal bone mineralization in AIS, which may shed light on etiopathogenesis of AIS.\u2014Chen, H., Zhang, J., Wang, Y., Cheuk, K.\u2010Y., Hung, A. L. H., Lam, T.\u2010P., Qiu, Y., Feng, J. Q., Lee, W. Y. W., Cheng, J. C. Y. Abnormal lacuno\u2010canalicular network and negative correlation between serum osteocalcin and Cobb angle indicate abnormal osteocyte function in adolescent idiopathic scoliosis. FASEB J. 33, 13882\u201013892 (2019).\n                    <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"https:\/\/www.fasebj.org\">www.fasebj.org<\/jats:ext-link>\n                  <\/jats:p>","DOI":"10.1096\/fj.201901227r","type":"journal-article","created":{"date-parts":[[2019,10,18]],"date-time":"2019-10-18T15:16:41Z","timestamp":1571411801000},"page":"13882-13892","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["Abnormal lacuno\u2010canalicular network and negative correlation between serum osteocalcin and Cobb angle indicate abnormal osteocyte function in adolescent idiopathic scoliosis"],"prefix":"10.1096","volume":"33","author":[{"given":"Huanxiong","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Spine and Osteopathic Surgery The First Affiliated Hospital of Hainan Medical University Hai\u2010kou China"},{"name":"Department of Orthopaedics and Traumatology S. H. Ho Scoliosis Research Laboratory, The Chinese University of Hong Kong Hong Kong China"},{"name":"Joint Scoliosis Research Center of The Chinese University of Hong Kong\u2010Nanjing University The Chinese University of Hong Kong Hong Kong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiajun","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Orthopaedics and Traumatology S. H. Ho Scoliosis Research Laboratory, The Chinese University of Hong Kong Hong Kong China"},{"name":"Joint Scoliosis Research Center of The Chinese University of Hong Kong\u2010Nanjing University The Chinese University of Hong Kong Hong Kong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yujia","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Orthopaedics and Traumatology S. H. 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Ho Scoliosis Research Laboratory, The Chinese University of Hong Kong Hong Kong China"},{"name":"Joint Scoliosis Research Center of The Chinese University of Hong Kong\u2010Nanjing University The Chinese University of Hong Kong Hong Kong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tsz\u2010Ping","family":"Lam","sequence":"additional","affiliation":[{"name":"Department of Orthopaedics and Traumatology S. H. Ho Scoliosis Research Laboratory, The Chinese University of Hong Kong Hong Kong China"},{"name":"Joint Scoliosis Research Center of The Chinese University of Hong Kong\u2010Nanjing University The Chinese University of Hong Kong Hong Kong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong","family":"Qiu","sequence":"additional","affiliation":[{"name":"Joint Scoliosis Research Center of The Chinese University of Hong Kong\u2010Nanjing University The Chinese University of Hong Kong Hong Kong China"},{"name":"Spine Surgery Nanjing Drum Tower Hospital Nanjing University Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jian Q.","family":"Feng","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences Texas A&amp;M College of Dentistry Dallas Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wayne Y. 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