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In this study, we show that miropin is also a specific and efficient inhibitor of plasmin; thus, it represents the first proteinaceous plasmin inhibitor of prokaryotic origin described to date. Miropin inhibits plasmin through the formation of a stable covalent complex triggered by cleavage of the Lys\n                    <jats:sup>368<\/jats:sup>\n                    \u2010Thr\n                    <jats:sup>369<\/jats:sup>\n                    (P2\u2010P1) reactive site bond with a stoichiometry of inhibition of 3.8 and an association rate constant (\n                    <jats:italic>\n                      k\n                      <jats:sub>ass<\/jats:sub>\n                    <\/jats:italic>\n                    ) of 3.3\u00a0\u00d7\u00a010\n                    <jats:sup>5<\/jats:sup>\n                    \u00a0M\n                    <jats:sup>\u22121<\/jats:sup>\n                    s\n                    <jats:sup>\u22121<\/jats:sup>\n                    . The inhibition of the fibrinolytic activity of plasmin was nearly as effective as that exerted by \u03b1\n                    <jats:sub>2<\/jats:sub>\n                    \u2010antiplasmin. Miropin also acted in vivo by reducing blood loss in a mice tail bleeding assay. Importantly, intact\n                    <jats:italic>T. forsythia<\/jats:italic>\n                    cells or outer membrane vesicles, both of which carry surface\u2010associated miropin, strongly inhibited plasmin. In intact bacterial cells, the antiplasmin activity of miropin protects envelope proteins from plasmin\u2010mediated degradation. In summary, in the environment of periodontal pockets, which are bathed in gingival crevicular fluid consisting of 70% of blood plasma, an abundance of\n                    <jats:italic>T. forsythia<\/jats:italic>\n                    in the bacterial biofilm can cause local inhibition of fibrinolysis, which could have possible deleterious effects on the tooth\u2010supporting structures of the periodontium.\n                  <\/jats:p>","DOI":"10.1096\/fj.201901490rr","type":"journal-article","created":{"date-parts":[[2019,11,26]],"date-time":"2019-11-26T04:49:11Z","timestamp":1574743751000},"page":"619-630","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Plasmin inhibition by bacterial serpin: Implications in gum disease"],"prefix":"10.1096","volume":"34","author":[{"given":"Alicja","family":"Sochaj\u2010Gregorczyk","sequence":"first","affiliation":[{"name":"Malopolska Center of Biotechnology Jagiellonian 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