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The data revealed that\u00a0miR\u2010196b\u20105p expression increased in primary or established marrow stromal progenitor cells after adipogenic treatment. Supplementing miR\u2010196b\u20105p in the progenitor cells stimulated adipogenic differentiation and lipogenesis, along with the induction of adipogenic and lipogenic factors. Conversely, inhibition of endogenous miR\u2010196b\u20105p blocked adipogenesis and lipogenesis. Tuberous sclerosis 1 (Tsc1) and transforming growth factor\u2010\u03b2 receptor 1 (TGFBR1) were demonstrated to be the direct target genes of\u00a0miR\u2010196b\u20105p. Supplementing miR\u2010196b\u20105p activity in progenitor cells reduced the protein level of TSC1 and activated mammalian target of rapamycin complex 1 (mTORC1) signaling. We further demonstrated that the perturbation of TSC1 in progenitor cells altered the trend of adipogenic differentiation and lipogenesis. Overexpression of Tsc1 or inactivation of mTORC1 signaling attenuated the stimulation of adipogenic differentiation and lipogenesis by miR\u2010196b\u20105p. Overexpression of Tgfbr1 also partially blocked the adipogenic effect of miR\u2010196b\u20105p. Further investigations demonstrated that zinc finger E\u2010box\u2010binding homeobox 1 (ZEB1) transcriptionally upregulated miR\u2010196b\u20105p expression. The current study suggests that miR\u2010196b\u20105p promotes adipogenic differentiation and lipogenesis in progenitor cells through targeting TSC1 and TGFBR1 and therefore regulating mTORC1 and TGF\u2010\u03b2 signaling.<\/jats:p>","DOI":"10.1096\/fj.201901562rr","type":"journal-article","created":{"date-parts":[[2020,7,27]],"date-time":"2020-07-27T10:37:08Z","timestamp":1595846228000},"page":"9207-9222","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["miR\u2010196b\u20105p controls adipocyte differentiation and lipogenesis through regulating mTORC1 and TGF\u2010\u03b2 signaling"],"prefix":"10.1096","volume":"34","author":[{"given":"Yaru","family":"Shi","sequence":"first","affiliation":[{"name":"NHC Key Lab of Hormones and Development, Tianjin Key Lab of Metabolic Diseases Chu Hsien\u2010I Memorial Hospital &amp; Institute of Endocrinology, Tianjin Medical University Tianjin 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