{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,2]],"date-time":"2026-11-02T00:28:40Z","timestamp":1793579320275,"version":"4.5.0"},"reference-count":65,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2019,10,2]],"date-time":"2019-10-02T00:00:00Z","timestamp":1569974400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000065","name":"National Institute of Neurological Disorders and Stroke","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000065","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000065","name":"National Institute of Neurological Disorders and Stroke","doi-asserted-by":"publisher","award":["2R01NS079702"],"award-info":[{"award-number":["2R01NS079702"]}],"id":[{"id":"10.13039\/100000065","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100005191","name":"Craig H. Neilsen Foundation","doi-asserted-by":"publisher","award":["476686"],"award-info":[{"award-number":["476686"]}],"id":[{"id":"10.13039\/100005191","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2019,12]]},"abstract":"<jats:p>\n                    More than half of spinal cord injury (SCI) cases occur in the cervical region, leading to respiratory dysfunction due to damaged neural circuitry that controls critically important muscles such as the diaphragm. The C3\u2010C5 spinal cord is the location of phrenic motor neurons (PhMNs) that are responsible for diaphragm activation; PhMNs receive bulbospinal excitatory drive predominately from supraspinal neurons of the rostral ventral respiratory group (rVRG). Cervical SCI results in rVRG axon damage, PhMN denervation, and consequent partial\u2010to\u2010complete paralysis of hemidiaphragm. In a rat model of C2 hemisection SCI, we expressed the axon guidance molecule, brain\u2010derived neurotrophic factor (BDNF), selectively at the location of PhMNs (ipsilateral to lesion) to promote directed growth of rVRG axons toward PhMN targets by performing intraspinal injections of adeno\u2010associated virus serotype 2 (AAV2)\u2010BDNF vector. AAV2\u2010BDNF promoted significant functional diaphragm recovery, as assessed by\n                    <jats:italic>in vivo<\/jats:italic>\n                    electromyography. Within the PhMN pool ipsilateral to injury, AAV2\u2010BDNF robustly increased sprouting of both spared contralateral\u2010originating rVRG axons and serotonergic fibers. Furthermore, AAV2\u2010BDNF significantly increased numbers of putative monosynaptic connections between PhMNs and these sprouting rVRG and serotonergic axons. These findings show that targeting circuit plasticity mechanisms involving the enhancement of synaptic inputs from spared axon populations is a powerful strategy for restoring respiratory function post\u2010SCI.\u2014Charsar, B. A., Brinton, M. A., Locke, K., Chen, A. Y., Ghosh, B., Urban, M. W., Komaravolu, S., Krishnamurthy, K., Smit, R., Pasinelli, P., Wright, M. C., Smith, G. M., Lepore, A. C. AAV2\u2010BDNF promotes respiratory axon plasticity and recovery of diaphragm function following spinal cord injury. FASEB J. 33, 13775\u201013793 (2019).\n                    <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"https:\/\/www.fasebj.org\">www.fasebj.org<\/jats:ext-link>\n                  <\/jats:p>","DOI":"10.1096\/fj.201901730r","type":"journal-article","created":{"date-parts":[[2019,10,2]],"date-time":"2019-10-02T17:52:06Z","timestamp":1570038726000},"page":"13775-13793","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":28,"title":["AAV2\u2010BDNF promotes respiratory axon plasticity and recovery of diaphragm function following spinal cord injury"],"prefix":"10.1096","volume":"33","author":[{"given":"Brittany A.","family":"Charsar","sequence":"first","affiliation":[{"name":"Department of Neuroscience Vickie and Jack Farber Institute for Neuroscience, Sidney Kimmel Medical College, Jefferson College of Life Sciences, Thomas Jefferson University Philadelphia Pennsylvania 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Life Sciences, Thomas Jefferson University Philadelphia Pennsylvania USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karthik","family":"Krishnamurthy","sequence":"additional","affiliation":[{"name":"Department of Neuroscience Jefferson Weinberg Amyotrophic Lateral Sclerosis (ALS) Center, Sidney Kimmel Medical College, Jefferson College of Life Sciences, Thomas Jefferson University Philadelphia Pennsylvania USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rupert","family":"Smit","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Cell Biology, Department of Neuroscience Shriners Hospitals Pediatric Research Center, Lewis Katz School of Medicine at Temple University Philadelphia Pennsylvania USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Piera","family":"Pasinelli","sequence":"additional","affiliation":[{"name":"Department of Neuroscience Jefferson Weinberg Amyotrophic Lateral Sclerosis (ALS) Center, Sidney Kimmel Medical College, Jefferson College of Life Sciences, Thomas Jefferson University Philadelphia Pennsylvania USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Megan C.","family":"Wright","sequence":"additional","affiliation":[{"name":"Department of Biology Arcadia University Philadelphia Pennsylvania USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"George M.","family":"Smith","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Cell Biology, Department of Neuroscience Shriners Hospitals Pediatric Research Center, Lewis Katz School of Medicine at Temple University Philadelphia Pennsylvania USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angelo C.","family":"Lepore","sequence":"additional","affiliation":[{"name":"Department of Neuroscience Vickie and Jack Farber Institute for Neuroscience, Sidney Kimmel Medical College, Jefferson College of Life Sciences, Thomas Jefferson University Philadelphia 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