{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,23]],"date-time":"2026-06-23T03:31:45Z","timestamp":1782185505427,"version":"3.54.5"},"reference-count":41,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2020,2,14]],"date-time":"2020-02-14T00:00:00Z","timestamp":1581638400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["NO. 30700877 and 81171675"],"award-info":[{"award-number":["NO. 30700877 and 81171675"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2020,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Chondrocyte apoptosis plays an important role in the developmental dysplasia of the hip (DDH) development. It has been found that WNT1 inducible signaling pathway protein 2 (WISP\u20102) and peroxisome proliferator\u2010activated receptor \u03b3 (PPAR\u03b3) are involved in cell apoptosis. In this study, we performed the straight\u2010leg swaddling DDH rat model and we found that cartilage degradation and chondrocyte apoptosis were remarkably increased in DDH rats in vivo\n                    <jats:italic>.<\/jats:italic>\n                    Moreover, we found that WISP\u20102 was upregulated in hip acetabular cartilage of DDH rats compared to control rats. Next, the effects of WISP\u20102 on chondrocyte apoptosis and its possible underlying mechanism were examined in vitro. The lentivirus\u2010mediated gain\u2010 and loss\u2010of\u2010function experiments of WISP\u20102 and peroxisome proliferator\u2010activated receptor \u03b3 (PPAR\u03b3) for cell viability and apoptosis were performed in primary rat chondrocytes. The results showed that the overexpression of WISP\u20102 induced chondrocyte apoptosis, and knockdown of WISP\u20102 could suppress the chondrocyte apoptosis induced by advanced glycation end products (AGE). Additionally, WISP\u20102 could negatively regulate the expression of PPAR\u03b3 in chondrocytes. Moreover, the knockdown of PPAR\u03b3 promoted chondrocyte apoptosis and overexpression of PPAR\u03b3 abated the increased apoptosis and decreased cell viability of chondrocytes induced by WISP\u20102. This study demonstrated that WISP\u20102 might contribute to chondrocyte apoptosis of hip acetabular cartilage through regulating PPAR\u03b3 expression and activation, which may play an important role in the development of DDH.\n                  <\/jats:p>","DOI":"10.1096\/fj.201901915r","type":"journal-article","created":{"date-parts":[[2020,2,14]],"date-time":"2020-02-14T09:33:41Z","timestamp":1581672821000},"page":"4904-4917","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["WISP\u20102, an upregulated gene in hip cartilage from the DDH model rats, induces chondrocyte apoptosis through PPAR\u03b3 in vitro"],"prefix":"10.1096","volume":"34","author":[{"given":"Xianglu","family":"Ji","sequence":"first","affiliation":[{"name":"Department of Hand and Foot Surgery Shengjing Hospital of China Medical University Shenyang People's Republic of 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