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More recent work has indicated that MRN may also impact on the duration of mitosis. We show here that RAD50\u2010deficient fibroblasts exhibit a marked delay in mitotic progression that can be rescued by lentiviral transduction of RAD50. The delay was observed throughout all mitotic phases in live cell imaging using GFP\u2010labeled H2B as a fluorescent marker. In complementation assays with RAD50 phosphorylation mutants, modifications at Ser635 had little effect on mitotic progression. By contrast with RAD50, fibroblast strains deficient in ATM or NBN did not show a significant slowing of mitotic progression. Ataxia\u2010telangiectasia\u2010like disorder (ATLD) fibroblasts with nuclease\u2010deficient MRE11A (p.W210C) tended to show slower mitosis, though by far not as significant as RAD50\u2010deficient cells. Inhibitor studies indicated that ATM kinase activity might not grossly impact on mitotic progression, while treatment with MRE11A inhibitor PFM39 modestly prolonged mitosis. Inhibition of ATR kinase significantly prolonged mitosis but this effect was mostly independent of RAD50 status. Taken together, our data unravel a mitotic role of RAD50 that can be separated from its known functions in DNA repair.<\/jats:p>","DOI":"10.1096\/fj.201902318r","type":"journal-article","created":{"date-parts":[[2019,12,31]],"date-time":"2019-12-31T04:20:19Z","timestamp":1577766019000},"page":"2812-2820","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["RAD50 regulates mitotic progression independent of DNA repair functions"],"prefix":"10.1096","volume":"34","author":[{"given":"Lea","family":"V\u00f6lkening","sequence":"first","affiliation":[{"name":"Gynaecology Research Unit Hannover Medical School Hannover Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anna","family":"Vatselia","sequence":"additional","affiliation":[{"name":"Gynaecology Research Unit Hannover Medical School Hannover 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