{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T03:15:41Z","timestamp":1791429341772,"version":"4.3.3"},"reference-count":64,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2020,1,9]],"date-time":"2020-01-09T00:00:00Z","timestamp":1578528000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000050","name":"National Heart, Lung, and Blood Institute","doi-asserted-by":"publisher","award":["R01HL143017"],"award-info":[{"award-number":["R01HL143017"]}],"id":[{"id":"10.13039\/100000050","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000050","name":"National Heart, Lung, and Blood Institute","doi-asserted-by":"publisher","award":["R01HL127338"],"award-info":[{"award-number":["R01HL127338"]}],"id":[{"id":"10.13039\/100000050","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100005640","name":"Flight Attendant Medical Research Institute","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100005640","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100005831","name":"Foundation for Anesthesia Education and Research","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100005831","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000968","name":"American Heart Association","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000968","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2020,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Pulmonary edema associated with increased vascular permeability is a severe complication of\n                    <jats:italic>Pseudomonas (P.) aeruginosa<\/jats:italic>\n                    \u2010induced acute lung injury. The mechanisms underlying\n                    <jats:italic>P aeruginosa<\/jats:italic>\n                    \u2010induced vascular permeability are not well understood. In the present study, we investigated the role of neuronal Wiskott Aldrich syndrome protein (N\u2010WASP) in modulating\n                    <jats:italic>P aeruginosa\u2010<\/jats:italic>\n                    induced vascular permeability. Using lung microvascular endothelial and alveolar epithelial cells, we demonstrated that N\u2010WASP downregulation attenuated\n                    <jats:italic>P aeruginosa<\/jats:italic>\n                    \u2010induced actin stress fiber formation and prevented paracellular permeability.\n                    <jats:italic>P aeruginosa<\/jats:italic>\n                    \u2010induced dissociation between VE\u2010cadherin and \u03b2\u2010catenin, but increased association between N\u2010WASP and VE\u2010cadherin, suggesting a role for N\u2010WASP in promoting\n                    <jats:italic>P aeruginosa<\/jats:italic>\n                    \u2010induced adherens junction rupture.\n                    <jats:italic>P aeruginosa<\/jats:italic>\n                    increased N\u2010WASP\u2010Y256 phosphorylation, which required the activation of Rho GTPase and focal adhesion kinase. Increased N\u2010WASP\u2010Y256 phosphorylation promotes N\u2010WASP and integrin \u03b1V\u03b26 association as well as TGF\u2010\u03b2\u2010mediated permeability across alveolar epithelial cells. Inhibition of N\u2010WASP\u2010Y256 phosphorylation by N\u2010WASP\u2010Y256F overexpression blocked N\u2010WASP effects in\n                    <jats:italic>P aeruginosa\u2010<\/jats:italic>\n                    induced actin stress fiber formation and increased paracellular permeability. In vivo, N\u2010WASP knockdown attenuated the development of pulmonary edema and improved survival in a mouse model of\n                    <jats:italic>P aeruginosa<\/jats:italic>\n                    pneumonia. Together, our data demonstrate that N\u2010WASP plays an essential role in\n                    <jats:italic>P aeruginosa<\/jats:italic>\n                    \u2010induced vascular permeability and pulmonary edema through the modulation of actin cytoskeleton dynamics.\n                  <\/jats:p>","DOI":"10.1096\/fj.201902915r","type":"journal-article","created":{"date-parts":[[2020,1,9]],"date-time":"2020-01-09T04:59:53Z","timestamp":1578545993000},"page":"3305-3317","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Neuronal Wiskott\u2010Aldrich syndrome protein regulates\n                    <i>Pseudomonas aeruginosa<\/i>\n                    \u2010induced lung vascular permeability through the modulation of actin cytoskeletal dynamics"],"prefix":"10.1096","volume":"34","author":[{"given":"Pulin","family":"Che","sequence":"first","affiliation":[{"name":"Department of Anesthesiology and Perioperative Medicine University of Alabama at Birmingham Birmingham AL USA"},{"name":"Molecular and Translational Biomedicine University of Alabama at Birmingham Birmingham AL USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brant M.","family":"Wagener","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology and Perioperative Medicine University of Alabama at Birmingham Birmingham AL USA"},{"name":"Molecular and Translational Biomedicine University of Alabama at Birmingham Birmingham AL USA"},{"name":"Divisions of Critical Care University of Alabama at Birmingham Birmingham AL USA"},{"name":"Center for Free Radical Biology University of Alabama at Birmingham Birmingham AL USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xueke","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases Affiliated Hospital of Guizhou Medical University Guiyang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angela P.","family":"Brandon","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology and Perioperative Medicine University of Alabama at Birmingham Birmingham AL USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cilina A.","family":"Evans","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology and Perioperative Medicine University of Alabama at Birmingham Birmingham AL USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guo\u2010Qiang","family":"Cai","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology and Perioperative Medicine University of Alabama at Birmingham Birmingham AL USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rui","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Genetics University of Alabama at Birmingham Birmingham AL USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhi\u2010Xiang","family":"Xu","sequence":"additional","affiliation":[{"name":"Comprehensive Cancer Center University of Alabama at Birmingham Birmingham AL USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaosi","family":"Han","sequence":"additional","affiliation":[{"name":"Department of Neurology University of Alabama at Birmingham Birmingham AL USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean\u2010Francois","family":"Pittet","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology and Perioperative Medicine University of Alabama at Birmingham Birmingham AL USA"},{"name":"Divisions of Critical Care University of Alabama at Birmingham Birmingham AL USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiang","family":"Ding","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology and Perioperative Medicine University of Alabama at Birmingham Birmingham AL USA"},{"name":"Molecular and Translational Biomedicine University of Alabama at Birmingham Birmingham AL USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,1,9]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM200005043421806"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa050333"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1097\/00003246-199711000-00026"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13054-014-0668-9"},{"key":"e_1_2_9_6_1","doi-asserted-by":"crossref","first-page":"1661","DOI":"10.1152\/jappl.1993.75.4.1661","article-title":"Alveolar epithelial injury and pleural empyema in acute P. aeruginosa pneumonia in anesthetized rabbits","volume":"75","author":"Wiener\u2010Kronish JP","year":"1993","journal-title":"Appl Physiol (1985)"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.0000134922.25721.d9"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.201812-2296OC"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1165\/rcmb.2007-0454OC"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1097\/ALN.0b013e3181f4171b"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.3390\/toxins10050185"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0074343"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M111.301440"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00441-014-1805-0"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.01515.2005"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2011.11.002"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI200420702"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm3492"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)80732-1"},{"key":"e_1_2_9_20_1","doi-asserted-by":"crossref","first-page":"699","DOI":"10.1242\/jcs.115.4.699","article-title":"N\u2010WASP activation by a beta1\u2010integrin\u2010dependent mechanism supports PI3K\u2010independent chemotaxis stimulated by urokinase\u2010type plasminogen activator","volume":"115","author":"Sturge J","year":"2002","journal-title":"J Cell Sci"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1189\/jlb.0804444"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1534-5807(02)00324-6"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm2069"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1097\/TA.0b013e318169cd3c"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1165\/rcmb.2008-0099OC"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(03)00120-X"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.3109\/10409230903277340"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.114.10.1801"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1165\/rcmb.2002-0092OC"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.201600102R"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.00390.2011"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.00173.2013"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.10-179218"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.18.5.2571"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexcr.2010.01.021"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M803645200"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M304685200"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep08453"},{"key":"e_1_2_9_39_1","first-page":"5336","article-title":"Promotion of malignant astrocytoma cell migration by osteopontin expressed in the normal brain: differences in integrin signaling during cell adhesion to osteopontin versus vitronectin","volume":"62","author":"Ding Q","year":"2002","journal-title":"Cancer Res"},{"key":"e_1_2_9_40_1","doi-asserted-by":"crossref","first-page":"188","DOI":"10.1164\/rccm.200304-478OC","article-title":"Transforming growth factor beta1 expression and activation is increased in the alcoholic rat lung","volume":"15","author":"Bechara RI","year":"2004","journal-title":"Am J Respir Crit Care Med"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.200304-478OC"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01431-07"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajpath.2013.01.026"},{"key":"e_1_2_9_44_1","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1055\/s-2000-9855","article-title":"Advances in infection control: ventilator\u2010associated pneumonia","volume":"21","author":"Hijazi MH","year":"2000","journal-title":"Semin Respir Crit Care Med"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.2000.279.3.L419"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)81010-7"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.17.9815"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1003939"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.00286.2018"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M115.668285"},{"key":"e_1_2_9_51_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200605012"},{"key":"e_1_2_9_52_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200212157"},{"key":"e_1_2_9_53_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.107.161067"},{"key":"e_1_2_9_54_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0063907"},{"key":"e_1_2_9_55_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.00094.2013"},{"key":"e_1_2_9_56_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.00178.2011"},{"key":"e_1_2_9_57_1","doi-asserted-by":"publisher","DOI":"10.1165\/rcmb.2006-0238OC"},{"key":"e_1_2_9_58_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)80545-0"},{"key":"e_1_2_9_59_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1462-5822.2001.00107.x"},{"key":"e_1_2_9_60_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamcr.2016.02.004"},{"key":"e_1_2_9_61_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijmm.2010.08.005"},{"key":"e_1_2_9_62_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2011.01606.x"},{"key":"e_1_2_9_63_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1006579"},{"key":"e_1_2_9_64_1","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.aab3881"},{"key":"e_1_2_9_65_1","doi-asserted-by":"publisher","DOI":"10.1165\/rcmb.2010-0397OC"}],"container-title":["The FASEB Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1096%2Ffj.201902915R","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1096\/fj.201902915R","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1096\/fj.201902915R","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/faseb.onlinelibrary.wiley.com\/doi\/pdf\/10.1096\/fj.201902915R","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T17:56:50Z","timestamp":1761674210000},"score":1,"resource":{"primary":{"URL":"https:\/\/faseb.onlinelibrary.wiley.com\/doi\/10.1096\/fj.201902915R"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,1,9]]},"references-count":64,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2020,2]]}},"alternative-id":["10.1096\/fj.201902915R"],"URL":"https:\/\/doi.org\/10.1096\/fj.201902915r","archive":["Portico"],"relation":{},"ISSN":["0892-6638","1530-6860"],"issn-type":[{"value":"0892-6638","type":"print"},{"value":"1530-6860","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,1,9]]},"assertion":[{"value":"2019-11-18","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-12-24","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-01-09","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}