{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T12:43:15Z","timestamp":1790772195215,"version":"4.1.0"},"reference-count":48,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2020,5,28]],"date-time":"2020-05-28T00:00:00Z","timestamp":1590624000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2020,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Thrombin converts fibrinogen to fibrin and activates blood and vascular cells in thrombo\u2010inflammatory diseases. Platelets are amplifiers of thrombin formation when activated by leukocyte\u2010 and vascular cell\u2010derived thrombin. CD36 on platelets acts as sensitizer for molecules with damage\u2010associated molecular patterns, thereby increasing platelet reactivity. Here, we investigated the role of CD36 in thrombin\u2010generation on human platelets, including selected patients with advanced chronic kidney disease (CKD). Platelets deficient in CD36 or blocked by anti\u2010CD36 antibody FA6.152 showed impaired thrombin generation triggered by thrombin in calibrated automated thrombography. Using platelets with congenital function defects, blocking antibodies, pharmacological inhibitors, and factor\u2010depleted plasma, CD36\u2010sensitive thrombin generation was dependent on FXI, fibrin, and platelet signaling via GPIb\u03b1 and SFKs. CD36\u2010deficiency or blocking suppressed thrombin\u2010induced platelet \u03b1IIb\u03b23 activation, granule exocytosis, binding of adhesion proteins and FV, FVIII, FIX, FX, but not anionic phospholipid exposure determined by flow cytometry. CD36 ligated specifically soluble fibrin, which recruited distinct coagulation factors via thiols. Selected patients with CKD showed elevated soluble fibrin plasma levels and enhanced thrombin\u2010induced thrombin generation, which was normalized by CD36 blocking. Thus, CD36 is an important amplifier of platelet\u2010dependent thrombin generation when exposure of anionic phospholipids is limited. This pathway might contribute to hypercoagulability in CKD.<\/jats:p>","DOI":"10.1096\/fj.201903189r","type":"journal-article","created":{"date-parts":[[2020,7,27]],"date-time":"2020-07-27T10:37:08Z","timestamp":1595846228000},"page":"9337-9357","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["CD36\u2010fibrin interaction propagates FXI\u2010dependent thrombin generation of human platelets"],"prefix":"10.1096","volume":"34","author":[{"given":"Mareike","family":"D\u00f6hrmann","sequence":"first","affiliation":[{"name":"Center for Thrombosis and Hemostasis (CTH) University Medical Center Johannes Gutenberg\u2010University Mainz Mainz Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephanie","family":"Makhoul","sequence":"additional","affiliation":[{"name":"Center for Thrombosis and Hemostasis (CTH) University Medical Center Johannes Gutenberg\u2010University Mainz Mainz Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kathrin","family":"Gross","sequence":"additional","affiliation":[{"name":"Center for Thrombosis and Hemostasis (CTH) University Medical Center Johannes Gutenberg\u2010University Mainz Mainz Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manuela","family":"Krause","sequence":"additional","affiliation":[{"name":"Deutsche Klinik f\u00fcr Diagnostik HELIOS Klinik Wiesbaden Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniele","family":"Pillitteri","sequence":"additional","affiliation":[{"name":"Deutsche Klinik f\u00fcr Diagnostik HELIOS Klinik Wiesbaden Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charis","family":"von Auer","sequence":"additional","affiliation":[{"name":"Third Department of Medicine University Medical Center Johannes Gutenberg\u2010University Mainz Mainz Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ulrich","family":"Walter","sequence":"additional","affiliation":[{"name":"Center for Thrombosis and Hemostasis (CTH) University Medical Center Johannes Gutenberg\u2010University Mainz Mainz Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jens","family":"Lutz","sequence":"additional","affiliation":[{"name":"Section of Nephrology, I. 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