{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,25]],"date-time":"2026-02-25T08:51:19Z","timestamp":1772009479559,"version":"3.50.1"},"reference-count":31,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2020,8,17]],"date-time":"2020-08-17T00:00:00Z","timestamp":1597622400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2020,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Erectile dysfunction (ED) is a common comorbidity in males with diabetes mellitus (DM), whose pathogenesis might be induced by dysregulation of corpus cavernosum smooth muscle cells (CCSMCs). Gene Expression Omnibus repository\u2010based analysis identified the differentially expressed PDCD4 in DM rats. PDCD4 has also been determined as a putative gene under the regulatory control of microRNA\u201021\u20105p (miR\u201021\u20105p). This study aimed to further determine the functional role of miR\u201021\u20105p in CCSMCs in a rat model of diabetes mellitus\u2010induced erectile dysfunction (DMED). CCSMCs were isolated from penile cavernous tissue and cultured in high glucose (HG) medium. The expression of miR\u201021\u20105p and\/or PDCD4 was altered in CCSMCs, as directly or indirectly measured by CCK\u20108 assay, flow cytometry, and TUNEL assays. Furthermore, exosomes were isolated from mesenchymal stem cells (MSCs) transfected with miR\u201021\u20105p mimic or miR\u201021\u20105p inhibitor and co\u2010cultured with CCSMCs. DMED rats were injected with lentivirus carrying PDCD4\/siRNA\u2010PDCD4 plasmids, or exosomes from MSCs containing miR\u201021\u20105p\u2010agomir to explore their roles in vivo. The experimental data validated that PDCD4 was upregulated in cavernous tissue of DMED rats. miR\u201021\u20105p targeted and inhibited PDCD4. miR\u201021\u20105p was enriched in MSC\u2010exosomes. Moreover, PDCD4 downregulation, miR\u201021\u20105p elevation or MSC\u2010derived exosomal miR\u201021\u20105p reduced apoptosis and enhanced proliferation of CCSMCs cultured in HG medium. PDCD4 silencing or miR\u201021\u20105p\u2010containing MSC\u2010exosomes improved erectile function and smooth muscle density in DMED rats. Collectively, our findings suggested that MSC\u2010derived exosomal miR\u201021\u20105p suppressed PDCD4 expression and ED in rats with DM.<\/jats:p>","DOI":"10.1096\/fj.202000102rr","type":"journal-article","created":{"date-parts":[[2020,8,28]],"date-time":"2020-08-28T00:55:27Z","timestamp":1598576127000},"page":"13345-13360","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":41,"title":["Mesenchymal stem cells\u2010derived exosomal microRNA\u201021\u20105p downregulates PDCD4 and ameliorates erectile dysfunction in a rat model of diabetes mellitus"],"prefix":"10.1096","volume":"34","author":[{"given":"Wei","family":"Huo","sequence":"first","affiliation":[{"name":"Department of Urology China\u2010Japan Union Hospital of Jilin University Changchun P. R. 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