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It was assumed that the adult lens had no source of immune cells and that the basement membrane capsule surrounding the lens was a barrier to immune cell migration. Yet, microfibril\u2010associated protein\u20101 (MAGP1)\u2010rich ciliary zonules that originate from the vasculature\u2010rich ciliary body and extend along the surface of the lens capsule, form a potential conduit for immune cells to the lens. In response to cornea debridement wounding, we find increased expression of MAGP1 throughout the central corneal stroma. The immune cells that populate this typically avascular region after wounding closely associate with this MAGP1\u2010rich matrix. These results suggest that MAGP1\u2010rich microfibrils support immune cell migration post\u2010injury. Using this cornea wound model, we investigated whether there is an immune response to the lens following cornea injury involving the lens\u2010associated MAGP1\u2010rich ciliary zonules. Our results provide the first evidence that following corneal wounding immune cells are activated to travel along zonule fibers that extend anteriorly along the equatorial surface of the lens, from where they migrate across the anterior lens capsule. These results demonstrate that lens\u2010associated ciliary zonules are directly involved in the lens immune response and suggest the ciliary body as a source of immune cells to the avascular lens.<\/jats:p>","DOI":"10.1096\/fj.202000289r","type":"journal-article","created":{"date-parts":[[2020,7,27]],"date-time":"2020-07-27T10:37:08Z","timestamp":1595846228000},"page":"9316-9336","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":31,"title":["An immune response to the avascular lens following wounding of the cornea involves ciliary zonule fibrils"],"prefix":"10.1096","volume":"34","author":[{"given":"JodiRae","family":"DeDreu","sequence":"first","affiliation":[{"name":"Department of Pathology, Anatomy and Cell Biology Sidney Kimmel Medical College Thomas Jefferson University Philadelphia PA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caitlin J.","family":"Bowen","sequence":"additional","affiliation":[{"name":"Department of Pathology, Anatomy and Cell Biology Sidney Kimmel Medical College Thomas Jefferson University Philadelphia PA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caitlin M.","family":"Logan","sequence":"additional","affiliation":[{"name":"Department of Pathology, Anatomy and Cell Biology Sidney Kimmel Medical College Thomas Jefferson University Philadelphia PA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sonali","family":"Pal\u2010Ghosh","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Cell Biology George Washington University School of Medicine and Health Sciences Washington DC USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paola","family":"Parlanti","sequence":"additional","affiliation":[{"name":"George Washington University Nanofabrication and Imaging Center George Washington University School of Medicine and Health Sciences Washington DC USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mary Ann","family":"Stepp","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Cell Biology George Washington University School of Medicine and Health Sciences Washington DC USA"},{"name":"Department of Ophthalmology George Washington University School of Medicine and Health Sciences Washington DC USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. 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