{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T15:59:22Z","timestamp":1790697562277,"version":"4.1.0"},"reference-count":63,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2020,4,13]],"date-time":"2020-04-13T00:00:00Z","timestamp":1586736000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2020,4,13]],"date-time":"2020-04-13T00:00:00Z","timestamp":1586736000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100000024","name":"Canadian Institutes of Health Research","doi-asserted-by":"publisher","award":["119871"],"award-info":[{"award-number":["119871"]}],"id":[{"id":"10.13039\/501100000024","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000024","name":"Canadian Institutes of Health Research","doi-asserted-by":"publisher","award":["84576"],"award-info":[{"award-number":["84576"]}],"id":[{"id":"10.13039\/501100000024","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000156","name":"Sant\u00e9","doi-asserted-by":"publisher","award":["112218"],"award-info":[{"award-number":["112218"]}],"id":[{"id":"10.13039\/501100000156","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2020,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Primary cilia (PC) are organelles that sense and respond to dynamic changes of the extracellular milieu through the regulation of target genes. By using the epididymis as a model system, we determined the contribution of primary cilia in the regulation of epithelial cell functions through the transduction of the Hedgehog (Hh) signaling pathway. Both Sonic (SHH) and Indian Hedgehog (IHH)\u00a0ligands were detected in epididymal epithelial cells by confocal microscopy and found secreted in the extracellular space. Gene expression profiling preformed on ciliated epithelial cells indicated that 153 and 1052 genes were differentially expressed following treatment with the Hh agonist SAG or the Hh antagonist cyclopamine (Cyclo), respectively. Strikingly, gene ontology analysis indicated that genes associated with immune response were the most affected following Hh modulation. The contribution of epididymal PC to canonical Hh pathway transduction was validated by ciliobrevin D treatment, which induced a significant decrease in PC length and a reduction in the expression Hh signaling targets. Such findings bring us closer to a molecular understanding of the subtle immune balance observed in some epithelia, including the epididymis and the intestine, which are organs featuring both tolerance toward autoimmune spermatozoa (or commensal bacteria) and defense against pathogens.<\/jats:p>","DOI":"10.1096\/fj.202000328r","type":"journal-article","created":{"date-parts":[[2020,4,13]],"date-time":"2020-04-13T17:04:46Z","timestamp":1586797486000},"page":"7593-7609","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Hedgehog signaling pathway regulates gene expression profile of epididymal principal cells through the primary cilium"],"prefix":"10.1096","volume":"34","author":[{"given":"Laura","family":"Girardet","sequence":"first","affiliation":[{"name":"Faculty of Medicine Department of Obstetrics, Gynecology and Reproduction Universit\u00e9 Laval, CHU de Qu\u00e9bec Research Center (CHUL) Quebec City QC Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Agathe","family":"Bernet","sequence":"additional","affiliation":[{"name":"Faculty of Medicine Department of Obstetrics, Gynecology and Reproduction Universit\u00e9 Laval, CHU de Qu\u00e9bec Research Center (CHUL) Quebec City QC Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ez\u00e9quiel","family":"Calvo","sequence":"additional","affiliation":[{"name":"Faculty of Medicine Department of Obstetrics, Gynecology and Reproduction Universit\u00e9 Laval, CHU de Qu\u00e9bec Research Center (CHUL) Quebec City QC Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Denis","family":"Soulet","sequence":"additional","affiliation":[{"name":"Faculty of Pharmacy Universit\u00e9 Laval, CHU de Qu\u00e9bec Research Center (CHUL) Quebec City QC Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charles","family":"Joly-Beauparlant","sequence":"additional","affiliation":[{"name":"Computational Biology Laboratory Research Centre Faculty of Medicine Laval University Quebec City QC Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arnaud","family":"Droit","sequence":"additional","affiliation":[{"name":"Computational Biology Laboratory Research Centre Faculty of Medicine Laval University Quebec City QC Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel G.","family":"Cyr","sequence":"additional","affiliation":[{"name":"Faculty of Medicine Department of Obstetrics, Gynecology and Reproduction Universit\u00e9 Laval, CHU de Qu\u00e9bec Research Center (CHUL) Quebec City QC Canada"},{"name":"Laboratory for Reproductive Toxicology INRS\u2010Institut Armand\u2010Frappier Universit\u00e9 du Qu\u00e9bec Laval QC Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cl\u00e9mence","family":"Belleann\u00e9e","sequence":"additional","affiliation":[{"name":"Faculty of Medicine Department of Obstetrics, Gynecology and Reproduction Universit\u00e9 Laval, CHU de Qu\u00e9bec Research Center (CHUL) Quebec City QC Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,4,13]]},"reference":[{"key":"e_1_2_13_2_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.050377"},{"key":"e_1_2_13_3_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1124534"},{"key":"e_1_2_13_4_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra1010172"},{"key":"e_1_2_13_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00441-016-2523-6"},{"key":"e_1_2_13_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature10936"},{"key":"e_1_2_13_7_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0909900107"},{"key":"e_1_2_13_8_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.1708-15.2015"},{"key":"e_1_2_13_9_1","doi-asserted-by":"publisher","DOI":"10.1161\/HYPERTENSIONAHA.117.09584"},{"key":"e_1_2_13_10_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1321425111"},{"key":"e_1_2_13_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.2011"},{"key":"e_1_2_13_12_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.166892"},{"key":"e_1_2_13_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/andr.12650"},{"key":"e_1_2_13_14_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0068521"},{"key":"e_1_2_13_15_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/dey276"},{"key":"e_1_2_13_16_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.233239"},{"key":"e_1_2_13_17_1","doi-asserted-by":"publisher","DOI":"10.4103\/1008-682X.153540"},{"key":"e_1_2_13_18_1","doi-asserted-by":"publisher","DOI":"10.2164\/jandrol.05114"},{"key":"e_1_2_13_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1939-4640.2004.tb02822.x"},{"key":"e_1_2_13_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb3091"},{"key":"e_1_2_13_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2003.12.015"},{"key":"e_1_2_13_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1939-4640.2002.tb02344.x"},{"key":"e_1_2_13_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2019"},{"key":"e_1_2_13_24_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/29.9.e45"},{"key":"e_1_2_13_25_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.10.2.634"},{"key":"e_1_2_13_26_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.R112.356006"},{"key":"e_1_2_13_27_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod63.6.1825"},{"key":"e_1_2_13_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tice.2012.10.003"},{"key":"e_1_2_13_29_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.E10-12-0994"},{"key":"e_1_2_13_30_1","doi-asserted-by":"publisher","DOI":"10.1359\/jbmr.070503"},{"key":"e_1_2_13_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2012.08.019"},{"key":"e_1_2_13_32_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.60.4.1295-1301.1992"},{"key":"e_1_2_13_33_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10565-017-9401-1"},{"key":"e_1_2_13_34_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201703196"},{"key":"e_1_2_13_35_1","doi-asserted-by":"crossref","first-page":"730","DOI":"10.4103\/1008-682X.155532","article-title":"Extracellular microRNAs from the epididymis as potential mediators of cell\u2010to\u2010cell communication","volume":"17","author":"Belleann\u00e9e C","year":"2015","journal-title":"Asian J Androl"},{"key":"e_1_2_13_36_1","doi-asserted-by":"publisher","DOI":"10.4161\/spmg.1.4.18948"},{"key":"e_1_2_13_37_1","doi-asserted-by":"publisher","DOI":"10.2164\/jandrol.111.012971"},{"key":"e_1_2_13_38_1","doi-asserted-by":"publisher","DOI":"10.1679\/aohc.47.137"},{"key":"e_1_2_13_39_1","doi-asserted-by":"publisher","DOI":"10.1002\/aja.1001540104"},{"key":"e_1_2_13_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mod.2013.12.002"},{"key":"e_1_2_13_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2012.07.010"},{"key":"e_1_2_13_42_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.183541"},{"key":"e_1_2_13_43_1","doi-asserted-by":"publisher","DOI":"10.1038\/oncsis.2014.27"},{"key":"e_1_2_13_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2007.03.004"},{"key":"e_1_2_13_45_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrg2774"},{"key":"e_1_2_13_46_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0505328102"},{"key":"e_1_2_13_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2019.11.015"},{"key":"e_1_2_13_48_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.e16-03-0189"},{"issue":"10","key":"e_1_2_13_49_1","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0013054","article-title":"cDNA microarray gene expression profiling of hedgehog signaling pathway inhibition in human colon cancer cells","volume":"5","author":"Shi T","year":"2010","journal-title":"PLoS One"},{"key":"e_1_2_13_50_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.32130"},{"key":"e_1_2_13_51_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1207273"},{"key":"e_1_2_13_52_1","doi-asserted-by":"publisher","DOI":"10.2337\/db10-1628"},{"key":"e_1_2_13_53_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0075225"},{"key":"e_1_2_13_54_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbi.2009.09.016"},{"key":"e_1_2_13_55_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0043338"},{"key":"e_1_2_13_56_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0075317"},{"key":"e_1_2_13_57_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2018.07.003"},{"key":"e_1_2_13_58_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M113.532234"},{"key":"e_1_2_13_59_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.00413-12"},{"key":"e_1_2_13_60_1","doi-asserted-by":"publisher","DOI":"10.1136\/gut.38.3.414"},{"key":"e_1_2_13_61_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1097-2765(02)00708-6"},{"key":"e_1_2_13_62_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biocel.2007.07.003"},{"key":"e_1_2_13_63_1","first-page":"339","article-title":"Urogenital Infection as a Risk Factor for Male Infertility","volume":"114","author":"Schuppe H\u2010C","year":"2017","journal-title":"Dtsch Arztebl Int"},{"key":"e_1_2_13_64_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0272.2007.00819.x"}],"container-title":["The FASEB Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1096%2Ffj.202000328R","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/faseb.onlinelibrary.wiley.com\/doi\/pdf\/10.1096\/fj.202000328R","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/faseb.onlinelibrary.wiley.com\/doi\/full-xml\/10.1096\/fj.202000328R","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/faseb.onlinelibrary.wiley.com\/doi\/pdf\/10.1096\/fj.202000328R","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T15:02:04Z","timestamp":1790694124000},"score":1,"resource":{"primary":{"URL":"https:\/\/faseb.onlinelibrary.wiley.com\/doi\/10.1096\/fj.202000328R"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,4,13]]},"references-count":63,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2020,6]]}},"alternative-id":["10.1096\/fj.202000328R"],"URL":"https:\/\/doi.org\/10.1096\/fj.202000328r","archive":["Portico"],"relation":{},"ISSN":["0892-6638","1530-6860"],"issn-type":[{"value":"0892-6638","type":"print"},{"value":"1530-6860","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,4,13]]},"assertion":[{"value":"2020-02-12","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-03-23","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-04-13","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}