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Conventional\n                    <jats:italic>Alox15<\/jats:italic>\n                    <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    mice are viable, develop normally but do not exhibit a major defective erythropoietic phenotype. To characterize the putative in vivo relevance of Alox15 for red blood cell development, we explored the impact of systemic inactivation of the\n                    <jats:italic>Alox15<\/jats:italic>\n                    gene on mouse erythropoiesis. We found that\n                    <jats:italic>Alox15<\/jats:italic>\n                    <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    mice exhibited reduced erythrocyte counts, elevated reticulocyte counts and red cell hyperchromia. The structure of the plasma membrane of\n                    <jats:italic>Alox15<\/jats:italic>\n                    <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    erythrocytes is altered and a significant share of the red cells was present as echinocytes and\/or acanthocytes. An increased share of the Alox15\n                    <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    erythrocytes cells were annexin V positive, which indicates a loss of plasma membrane asymmetry. Erythrocytes of\n                    <jats:italic>Alox15<\/jats:italic>\n                    <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    mice were more susceptible to osmotic hemolysis and exhibited a reduced ex vivo life span. When we transgenically expressed human ALOX15 in\n                    <jats:italic>Alox15<\/jats:italic>\n                    <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    mice under the control of the aP2 promoter the defective erythropoietic system was rescued and the impaired osmotic resistance was normalized. Together these data suggest the involvement Alox15 in the maturational remodeling of the plasma membrane during red cell development.\n                  <\/jats:p>","DOI":"10.1096\/fj.202000408rr","type":"journal-article","created":{"date-parts":[[2020,9,18]],"date-time":"2020-09-18T03:15:14Z","timestamp":1600398914000},"page":"14318-14335","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Systemic deficiency of mouse arachidonate 15\u2010lipoxygenase induces defective erythropoiesis and transgenic expression of the human enzyme rescues this phenotype"],"prefix":"10.1096","volume":"34","author":[{"given":"Marlena","family":"Rademacher","sequence":"first","affiliation":[{"name":"Charit\u00e9 \u2010 Universit\u00e4tsmedizin Berlin, corporate member of Freie Universit\u00e4t Berlin Humboldt\u2010Universit\u00e4t zu Berlin, and Berlin Institute of Health Institute of Biochemistry Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hartmut","family":"Kuhn","sequence":"additional","affiliation":[{"name":"Charit\u00e9 \u2010 Universit\u00e4tsmedizin Berlin, corporate member of Freie Universit\u00e4t Berlin Humboldt\u2010Universit\u00e4t zu Berlin, and Berlin Institute of Health Institute of Biochemistry Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Astrid","family":"Borchert","sequence":"additional","affiliation":[{"name":"Charit\u00e9 \u2010 Universit\u00e4tsmedizin Berlin, corporate member of Freie Universit\u00e4t Berlin Humboldt\u2010Universit\u00e4t zu Berlin, and Berlin Institute of Health Institute of Biochemistry Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,9,12]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2018.05.045"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1155\/2017\/4586068"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.2174\/1871527317666180425122557"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.3233\/JAD-2004-6206"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.redox.2019.101284"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2017.01.004"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1515\/hsz-2016-0102"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gene.2015.07.073"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2017.07.010"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200612116"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1742-4658.2007.05909.x"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/0304-4157(86)90006-7"},{"key":"e_1_2_9_14_1","first-page":"1237","article-title":"The occurrence of a lipoxygenase pathway in reticulocytes of various species","volume":"45","author":"Kroschwald P","year":"1986","journal-title":"Biomed Biochim Acta"},{"key":"e_1_2_9_15_1","first-page":"S42","article-title":"The erythroid arachidonate 15\u2010lipoxygenase in rat reticulocytes","volume":"49","author":"Schewe T","year":"1990","journal-title":"Biomed Biochim Acta"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.plipres.2018.09.002"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0090-6980(02)00036-9"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.38.23338"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbalip.2014.10.002"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1021\/cr200246d"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI115110"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(89)92528-X"},{"key":"e_1_2_9_23_1","first-page":"333","volume-title":"Proceedings in Life Sciences","author":"Rapoport S","year":"1985"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/9780470123041.ch6"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1979.tb13068.x"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1982.tb07031.x"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1994.tb06402.x"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)80241-X"},{"key":"e_1_2_9_29_1","first-page":"1129","article-title":"In vitro maturation of rabbit reticulocytes. 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