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Since 12\/15\u2010lipoxygenase (12\/15\u2010LOX)\u2010derived lipid mediators such as lipoxin A\n                    <jats:sub>4<\/jats:sub>\n                    (LXA\n                    <jats:sub>4<\/jats:sub>\n                    ) and protectin D1 (PD1) protect against corneal epithelial cell damage, the major cell types that express 12\/15\u2010LOX and contribute to the corneal wound healing process are of particular interest. Here, we found that eosinophils were the major cell type expressing 12\/15\u2010LOX during the corneal wound healing process. Eosinophils were recruited into the conjunctiva after corneal epithelium wounding, and eosinophil\u2010deficient and\/or eosinophil\u2010specific 12\/15\u2010LOX knockout mice showed delayed corneal wound healing compared with wild\u2010type mice. Liquid chromatography\u2010tandem mass spectrometry (LC\u2010MS\/MS)\u2010based mediator lipidomics revealed that a series of 12\/15\u2010LOX\u2010derived mediators were significantly decreased in eosinophil\u2010deficient mice and topical application of 17\u2010hydroxydocosahexaenoic acid (17\u2010HDoHE), a major 12\/15\u2010LOX\u2010derived product, restored the phenotype. These results indicate that 12\/15\u2010LOX\u2010expressing eosinophils, by locally producing pro\u2010resolving mediators, significantly contribute to the corneal wound healing process in the eye.\n                  <\/jats:p>","DOI":"10.1096\/fj.202000483r","type":"journal-article","created":{"date-parts":[[2020,7,28]],"date-time":"2020-07-28T12:55:04Z","timestamp":1595940904000},"page":"12492-12501","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":27,"title":["Eosinophils promote corneal wound healing via the 12\/15\u2010lipoxygenase pathway"],"prefix":"10.1096","volume":"34","author":[{"given":"Mamoru","family":"Ogawa","sequence":"first","affiliation":[{"name":"Laboratory for Metabolomics RIKEN Center for Integrative Medical Sciences (IMS) Yokohama Japan"},{"name":"Department of Ophthalmology Keio University School of Medicine Tokyo 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