{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T11:51:22Z","timestamp":1777636282307,"version":"3.51.4"},"reference-count":35,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2020,11,8]],"date-time":"2020-11-08T00:00:00Z","timestamp":1604793600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2020,11,8]],"date-time":"2020-11-08T00:00:00Z","timestamp":1604793600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100004607","name":"Natural Science Foundation of Guangxi Province","doi-asserted-by":"publisher","award":["2020GXNSFDA238007"],"award-info":[{"award-number":["2020GXNSFDA238007"]}],"id":[{"id":"10.13039\/501100004607","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2021,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The aberrant expression of long noncoding RNA (lncRNA) taurine\u2010upregulated gene 1 (TUG1) has been previously associated with myocardial ischemia\u2010reperfusion injury (MIRI), but the underlying molecular mechanisms remain elusive. The current study aimed to clarify the functional role of TUG1\/microRNA (miR)\u2010340\/histone deacetylase 4 (HDAC4)\/\u03b2\u2010catenin\/glucose transporter type 1 (GLUT1) axes in MIRI. The database\u2010based analyses performed predicted the downstream factors of lncRNA TUG1. In the MIRI mouse models and hypoxia\/reoxygenation (H\/R)\u2010induced cardiomyocyte models, the expression of TUG1\/miR\u2010340\/HDAC4\/\u03b2\u2010catenin\/GLUT1 was manipulated to examine their effects on the infarction area, cardiomyocyte viability and apoptosis employing the Evans blue\/TTC double staining, CCK\u20108 and TUNEL assays. Furthermore, the dual luciferase reporter and RIP assays verified the binding affinity of miR\u2010340 to TUG1 and HDAC4. Subsequently, a negative correlation between miR\u2010340 and TUG1 or HDAC4 expression was identified in myocardial tissues of MIRI mice and H\/R\u2010induced cardiomyocyte models, along with a positive correlation between TUG1 and HDAC4. Additionally, it was established that TUG1 bound to miR\u2010340, and miR\u2010340 targeted HDAC4. TUG1 upregulated HDAC4 expression, thereby promoting MIRI in the mouse models. HDAC4 was proven to repress the expression of \u03b2\u2010catenin and its target gene GLUT1. Moreover, the in vivo experiments validated that the inhibition of TUG1\/miR\u2010340\/HDAC4\/\u03b2\u2010catenin\/GLUT1 axes alleviated MIRI in mice. Collectively, the current study uncovered the role of TUG1\/miR\u2010340\/HDAC4\/\u03b2\u2010catenin\/GLUT1 axes in MIRI mouse models and H\/R\u2010induced cardiomyocyte models which may be a potential therapeutic target for MIRI treatment.<\/jats:p>","DOI":"10.1096\/fj.202000827rr","type":"journal-article","created":{"date-parts":[[2020,11,9]],"date-time":"2020-11-09T02:54:29Z","timestamp":1604890469000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["LncRNA TUG1 competitively binds to miR\u2010340 to accelerate myocardial ischemia\u2010reperfusion injury"],"prefix":"10.1096","volume":"35","author":[{"given":"Xianqiu","family":"Wu","sequence":"first","affiliation":[{"name":"Department of Cardio\u2010Thoracic Surgery the Fourth Affiliated Hospital of Guangxi Medical University Liuzhou P.R. 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