{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,20]],"date-time":"2026-01-20T01:06:22Z","timestamp":1768871182266,"version":"3.49.0"},"reference-count":45,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2020,7,15]],"date-time":"2020-07-15T00:00:00Z","timestamp":1594771200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000049","name":"National Institute on Aging","doi-asserted-by":"publisher","award":["AG031679"],"award-info":[{"award-number":["AG031679"]}],"id":[{"id":"10.13039\/100000049","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2020,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Testosterone stimulates iron\u2010dependent erythropoiesis and suppresses hepcidin. To clarify the role of iron in mediating testosterone's effects on erythropoiesis, we induced iron deficiency in mice by feeding low iron diet. Iron\u2010replete and iron\u2010deficient mice were treated weekly with testosterone propionate or vehicle for 3\u00a0weeks. Testosterone treatment increased red cell count in iron\u2010replete mice, but, surprisingly, testosterone reduced red cell count in iron\u2010deficient mice. Splenic stress erythropoiesis was stimulated in iron\u2010deficient mice relative to iron\u2010replete mice, and further increased by testosterone treatment, as indicated by the increase in red pulp area, the number of nucleated erythroblasts, and expression levels of TfR1, GATA1, and other erythroid genes. Testosterone treatment of iron\u2010deficient mice increased the ratio of early\u2010to\u2010late erythroblasts in the spleen and bone marrow, and serum LDH level, consistent with ineffective erythropoiesis. In iron\u2010deficient mice, erythropoietin levels were higher but erythropoietin\u2010regulated genes were generally downregulated relative to iron\u2010replete mice, suggesting erythropoietin resistance. Conclusion: Testosterone treatment stimulates splenic stress erythropoiesis in iron\u2010replete as well as iron\u2010deficient mice. However, testosterone worsens anemia in iron\u2010deficient mice because of ineffective erythropoiesis possibly due to erythropoietin resistance associated with iron deficiency. Iron plays an important role in mediating testosterone's effects on erythropoiesis.<\/jats:p>","DOI":"10.1096\/fj.202000920rr","type":"journal-article","created":{"date-parts":[[2020,7,15]],"date-time":"2020-07-15T10:51:23Z","timestamp":1594810283000},"page":"11672-11684","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["The role of iron in mediating testosterone's effects on erythropoiesis in mice"],"prefix":"10.1096","volume":"34","author":[{"given":"Wen","family":"Guo","sequence":"first","affiliation":[{"name":"Research Program in Men's Health: Aging and Metabolism The Boston Claude D. Pepper Older Americans Independence Center Brigham and Women's Hospital Harvard Medical School Boston MA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ramy","family":"Abou Ghayda","sequence":"additional","affiliation":[{"name":"Research Program in Men's Health: Aging and Metabolism The Boston Claude D. Pepper Older Americans Independence Center Brigham and Women's Hospital Harvard Medical School Boston MA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paul J.","family":"Schmidt","sequence":"additional","affiliation":[{"name":"Department of Pathology Boston Children's Hospital Harvard Medical School Boston MA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mark D.","family":"Fleming","sequence":"additional","affiliation":[{"name":"Department of Pathology Boston Children's Hospital Harvard Medical School Boston MA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shalender","family":"Bhasin","sequence":"additional","affiliation":[{"name":"Research Program in Men's Health: Aging and Metabolism The Boston Claude D. 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