{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,28]],"date-time":"2026-10-28T14:16:33Z","timestamp":1793196993519,"version":"4.5.0"},"reference-count":46,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2020,8,14]],"date-time":"2020-08-14T00:00:00Z","timestamp":1597363200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/100006510","name":"Duke University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100006510","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2020,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Inflammaging is associated with poor tissue regeneration observed in advanced age. Specifically, protracted inflammation after acute injury has been associated with decreased bone fracture healing and increased rates of nonunion in elderly patients. Here, we investigated the efficacy of using Maresin 1 (MaR1), an omega\u20103 fatty acid\u2010derived pro\u2010resolving agent, to resolve inflammation after tibial fracture injury and subsequently improving aged bone healing. Aged (24\u2010month\u2010old mice) underwent tibial fracture surgery and were either treated with vehicle or MaR1 3\u00a0days after injury. Fracture calluses were harvested 7\u00a0days, 14\u00a0days, 21\u00a0days, and 28\u00a0days after injury to investigate inflammatory response, cartilage development, bone deposition, and mechanical integrity, respectively. Healing bones from MaR1\u2010treated mice displayed decreased cartilage formation and increased bone deposition which resulted in increased structural stiffness and increased force to fracture in the later stages of repair. In the early stages, MaR1 treatment decreased the number of pro\u2010inflammatory macrophages within the fracture callus and decreased the level of inflammatory biomarkers in circulation. In tissue culture models, MaR1 treatment of bone marrow\u2010derived macrophages from aged mice protected cells form a pro\u2010inflammatory phenotype and induced an anti\u2010inflammatory fate. Furthermore, the secretome of MaR1\u2010treated bone marrow\u2010derived macrophages was identified as osteoinductive, enhancing osteoblast differentiation of bone marrow stromal cells. Our findings here identify resolution of inflammation, and MaR1 itself, to be a point of intervention to improve aged bone healing.<\/jats:p>","DOI":"10.1096\/fj.202001145r","type":"journal-article","created":{"date-parts":[[2020,8,14]],"date-time":"2020-08-14T12:30:58Z","timestamp":1597408258000},"page":"13521-13532","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":42,"title":["Maresin 1 resolves aged\u2010associated macrophage inflammation to improve bone regeneration"],"prefix":"10.1096","volume":"34","author":[{"given":"Rong","family":"Huang","sequence":"first","affiliation":[{"name":"Duke Molecular Physiology Institute Duke University Durham NC USA"},{"name":"Department of Orthopaedic Surgery Duke University Durham NC USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linda","family":"Vi","sequence":"additional","affiliation":[{"name":"Division of Physical Medicine and Rehabilitation University of Toronto 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