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In vitro, MDPC23 cells were treated with rapamycin (10\u00a0nmol\/L) and transfected with a lentivirus for short hairpin (shRNA)\u2010mediated silencing of the tuberous sclerosis complex (shTSC1) to inhibit and activate mTORC1, respectively. CCK8 assays, flow cytometry, Alizarin red S staining, ALP staining, qRT\u2010PCR, and western blot analysis were performed. TSC1\u2010conditional knockout (DMP1\u2010Cre\n                    <jats:sup>+<\/jats:sup>\n                    ; TSC1\n                    <jats:sup>f\/f<\/jats:sup>\n                    , hereafter CKO) mice and littermate control (DMP1\u2010Cre\n                    <jats:sup>\u2212<\/jats:sup>\n                    ; TSC1\n                    <jats:sup>f\/f<\/jats:sup>\n                    , hereafter WT) mice were generated. H&amp;E staining, immunofluorescence, and micro\u2010CT analysis were performed. Transcriptome sequencing analysis was used to screen the mechanism of this process. mTORC1 inactivation decreased the cell proliferation. The qRT\u2010PCR and western blot results showed that mineralization\u2010related genes and proteins were downregulated in mTORC1\u2010inactivated cells. Moreover, mTORC1 overactivation promoted cell proliferation and mineralization\u2010related gene and protein expression. In vivo, the micro\u2010CT results showed that DV\/TV and dentin thickness were higher in CKO mice than in controls and H&amp;E staining showed the same results. Mineralization\u2010related proteins expression was upregulated. Transcriptome sequencing analysis revealed that p53 pathway\u2010associated genes were differentially expressed in TSC1\u2010deficient cells. By inhibiting p53 alone or both mTORC1 and p53 with rapamycin and a p53 inhibitor, we elucidated that p53 acts downstream of mTORC1 and that mTORC1 thereby promotes odontoblast mineralization. Taken together, our findings demonstrate that the role of mTORC1 in odontoblast proliferation and mineralization, and confirm that mTORC1 upregulates odontoblast mineralization via the p53 pathway.\n                  <\/jats:p>","DOI":"10.1096\/fj.202002016r","type":"journal-article","created":{"date-parts":[[2021,1,28]],"date-time":"2021-01-28T20:18:30Z","timestamp":1611865110000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["mTORC1 promotes mineralization via p53 pathway"],"prefix":"10.1096","volume":"35","author":[{"given":"Xinghong","family":"Luo","sequence":"first","affiliation":[{"name":"Department of Stomatology Nanfang Hospital, Southern Medical University Guangzhou China"},{"name":"College of Stomatology Southern Medical University Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jingyao","family":"Yin","sequence":"additional","affiliation":[{"name":"Department of 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