{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,9]],"date-time":"2025-11-09T07:47:59Z","timestamp":1762674479260,"version":"build-2065373602"},"reference-count":76,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2021,1,28]],"date-time":"2021-01-28T00:00:00Z","timestamp":1611792000000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2021,1,28]],"date-time":"2021-01-28T00:00:00Z","timestamp":1611792000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000026","name":"Institute of Aging","doi-asserted-by":"publisher","award":["1R56AG062292\u201001"],"award-info":[{"award-number":["1R56AG062292\u201001"]}],"id":[{"id":"10.13039\/501100000026","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["R01GM120634"],"award-info":[{"award-number":["R01GM120634"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["NSF CBET 1802793","NSF 1445151","NRT 1449999"],"award-info":[{"award-number":["NSF CBET 1802793","NSF 1445151","NRT 1449999"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000097","name":"National Center for Research Resources","doi-asserted-by":"publisher","award":["5P20RR01647\u201011"],"award-info":[{"award-number":["5P20RR01647\u201011"]}],"id":[{"id":"10.13039\/100000097","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["8 P20 GM103476\u201011"],"award-info":[{"award-number":["8 P20 GM103476\u201011"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2021,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Alzheimer's disease (AD) is a progressive neurodegenerative disorder that affects cognition and memory. Recent advances have helped identify many clinical sub\u2010types in AD. Mounting evidence point toward structural polymorphism among fibrillar aggregates of amyloid\u2010\u03b2 (A\u03b2) to being responsible for the phenotypes and clinical manifestations. In the emerging paradigm of polymorphism and prion\u2010like propagation of aggregates in AD, the role of low molecular weight soluble oligomers, which are long known to be the primary toxic agents, in effecting phenotypes remains inconspicuous. In this study, we present the characterization of three soluble oligomers of A\u03b242, namely 14LPOs, 16LPOs, and GM1Os with discreet biophysical and biochemical properties generated using lysophosphatidyl glycerols and GM1 gangliosides. The results indicate that the oligomers share some biophysical similarities but display distinctive differences with GM1Os. Unlike the other two, GM1Os were observed to be complexed with the lipid upon isolation. It also differs mainly in detection by conformation\u2010sensitive dyes and conformation\u2010specific antibodies, temperature and enzymatic stability, and in the ability to propagate morphologically\u2010distinct fibrils. GM1Os also show distinguishable biochemical behavior with pronounced neuronal toxicity. Furthermore, all the oligomers induce cerebral amyloid angiopathy (CAA) and plaque burden in transgenic AD mice, which seems to be a consistent feature among all lipid\u2010derived oligomers, but 16LPOs and GM1Os displayed significantly higher effect than the others. These results establish a correlation between molecular features of A\u03b242 oligomers and their distinguishable effects in transgenic AD mice attuned by lipid characteristics, and therefore help bridge the knowledge gap in understanding how oligomer conformers could elicit AD phenotypes.<\/jats:p>","DOI":"10.1096\/fj.202002025rr","type":"journal-article","created":{"date-parts":[[2021,1,28]],"date-time":"2021-01-28T20:27:49Z","timestamp":1611865669000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Biophysical characteristics of lipid\u2010induced A\u03b2 oligomers correlate to distinctive phenotypes in transgenic mice"],"prefix":"10.1096","volume":"35","author":[{"given":"Jhinuk","family":"Saha","sequence":"first","affiliation":[{"name":"Department of Chemistry and Biochemistry School of Mathematics and Natural Sciences University of Southern Mississippi Hattiesburg MS USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dexter N.","family":"Dean","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biochemistry School of Mathematics and Natural Sciences University of Southern Mississippi Hattiesburg MS USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shailendra","family":"Dhakal","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biochemistry School of Mathematics and Natural Sciences University of Southern Mississippi Hattiesburg MS USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kelli A.","family":"Stockmal","sequence":"additional","affiliation":[{"name":"School of Polymer Science and Engineering University of Southern Mississippi Hattiesburg MS USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sarah E.","family":"Morgan","sequence":"additional","affiliation":[{"name":"School of Polymer Science and Engineering University of Southern Mississippi Hattiesburg MS USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kristy D.","family":"Dillon","sequence":"additional","affiliation":[{"name":"Center for Translational Research in Neurodegenerative Disease University of Florida Gainesville FL USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Munir F.","family":"Adamo","sequence":"additional","affiliation":[{"name":"Center for Translational Research in Neurodegenerative Disease University of Florida Gainesville FL USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yona","family":"Levites","sequence":"additional","affiliation":[{"name":"Center for Translational Research in Neurodegenerative Disease University of Florida Gainesville FL USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vijayaraghavan","family":"Rangachari","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biochemistry School of Mathematics and Natural Sciences University of Southern Mississippi Hattiesburg MS USA"},{"name":"Center for Molecular and Cellular Biosciences University of Southern Mississippi Hattiesburg MS USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,1,28]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.2001.81.2.741"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.22-05-01858.2002"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2005.01.040"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1834302100"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm2101"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.3233\/JAD-142136"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/1531-8249(199912)46:6<860::AID-ANA8>3.0.CO;2-M"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)65184-X"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep40787"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1105850"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2010.06.023"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1524807113"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-genet-120215-034943"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi500792f"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2013.08.035"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1093\/brain\/awv006"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1713215114"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1714966115"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature20814"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1021\/cn300030a"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1039\/C4CP05563B"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0018759"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biochi.2016.08.014"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/bs.irn.2015.05.008"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biochi.2017.03.019"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep13489"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-319-17344-3_3"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm1095-1062"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0197-4580(98)00032-3"},{"key":"e_1_2_9_31_1","first-page":"99","volume-title":"Handbook of Neurochemistry and Molecular Neurobiology: Neural Lipids","author":"Tettamanti G","year":"2010"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.37.22987"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1006\/abbi.2001.2304"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi982345o"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi0255874"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M100252200"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(96)01504-9"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.1995.0525"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi971843e"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2003.11.046"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi701213s"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.2005.03407.x"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M112.355156"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0111492"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.biochem.6b00161"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpj.2017.11.3778"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1063\/1.5082659"},{"key":"e_1_2_9_48_1","unstructured":"ChemesLB AlonsoLG NovalMG dePrat GayG.Circular Dichroism Techniques for the Analysis of Intrinsically Disordered Proteins and DomainsIn:UverskyV. DunkerA.(eds)Methods in Molecular Biology (Methods and Protocols)895Humana Press:Totowa NJ;2012https:\/\/doi.org\/10.1007\/978\u20101\u201061779\u2010927\u20103_22."},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpj.2017.03.013"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-60263-4"},{"key":"e_1_2_9_51_1","doi-asserted-by":"publisher","DOI":"10.1002\/bip.1978.360170602"},{"key":"e_1_2_9_52_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1113694"},{"key":"e_1_2_9_53_1","doi-asserted-by":"publisher","DOI":"10.1186\/2051-5960-1-38"},{"key":"e_1_2_9_54_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/24.6.963"},{"key":"e_1_2_9_55_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00438a031"},{"key":"e_1_2_9_56_1","doi-asserted-by":"publisher","DOI":"10.1194\/jlr.D800038-JLR200"},{"key":"e_1_2_9_57_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(97)00391-9"},{"key":"e_1_2_9_58_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13361-016-1474-1"},{"key":"e_1_2_9_59_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1745-7270.2007.00320.x"},{"key":"e_1_2_9_60_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M114.569004"},{"key":"e_1_2_9_61_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2015.04.004"},{"key":"e_1_2_9_62_1","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20090379"},{"key":"e_1_2_9_63_1","doi-asserted-by":"publisher","DOI":"10.2174\/138920312800785058"},{"key":"e_1_2_9_64_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13361-018-1975-1"},{"key":"e_1_2_9_65_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.2004.02533.x"},{"key":"e_1_2_9_66_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(96)01279-3"},{"key":"e_1_2_9_67_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1714966115"},{"key":"e_1_2_9_68_1","doi-asserted-by":"publisher","DOI":"10.3233\/JAD-2011-102100"},{"key":"e_1_2_9_69_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M404751200"},{"key":"e_1_2_9_70_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature20814"},{"key":"e_1_2_9_71_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40478-020-00911-y"},{"key":"e_1_2_9_72_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.biomac.7b01007"},{"key":"e_1_2_9_73_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1408900111"},{"key":"e_1_2_9_74_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4886(83)90271-6"},{"key":"e_1_2_9_75_1","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-67-2-255"},{"key":"e_1_2_9_76_1","first-page":"85","article-title":"Prion protein (PrP) deposits in the tectum of experimental Gerstmann\u2010Straussler\u2010Scheinker disease following intraocular inoculation","volume":"50","author":"Liberski PP","year":"2012","journal-title":"Folia Neuropathol"},{"key":"e_1_2_9_77_1","first-page":"1","article-title":"Abeta seeds and prions: how close the fit?","author":"Rasmussen J","year":"2017","journal-title":"Prion"}],"container-title":["The FASEB Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1096\/fj.202002025RR","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1096\/fj.202002025RR","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/faseb.onlinelibrary.wiley.com\/doi\/pdf\/10.1096\/fj.202002025RR","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/faseb.onlinelibrary.wiley.com\/doi\/pdf\/10.1096\/fj.202002025RR","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T23:34:26Z","timestamp":1761694466000},"score":1,"resource":{"primary":{"URL":"https:\/\/faseb.onlinelibrary.wiley.com\/doi\/10.1096\/fj.202002025RR"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,1,28]]},"references-count":76,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2021,2]]}},"alternative-id":["10.1096\/fj.202002025RR"],"URL":"https:\/\/doi.org\/10.1096\/fj.202002025rr","archive":["Portico"],"relation":{},"ISSN":["0892-6638","1530-6860"],"issn-type":[{"type":"print","value":"0892-6638"},{"type":"electronic","value":"1530-6860"}],"subject":[],"published":{"date-parts":[[2021,1,28]]},"assertion":[{"value":"2020-09-01","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-12-14","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-01-28","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e21318"}}