{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T23:26:04Z","timestamp":1793143564495,"version":"4.5.0"},"reference-count":38,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2021,4,3]],"date-time":"2021-04-03T00:00:00Z","timestamp":1617408000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2021,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    The elevated intestinal permeability due to mucosal barrier defects is not only secondary to inflammatory bowel disease but also precedes enteritis. Tetrandrine, a bisbenzyl isoquinoline alkaloid isolated from the dried roots of\n                    <jats:italic>Stephamis tetlandra<\/jats:italic>\n                    S. Moor, was previously demonstrated to ameliorate colitis induced by dextran sulfate sodium (DSS) in mice. Here, we investigate whether and how tetrandrine protects against the disruption of the intestinal epithelial barrier under colitis condition. The data show that oral administration of tetrandrine significantly counteracted the increase of intestinal permeability in DSS\u2010treated mice, enhanced the mRNA and protein expression of Occludin and Claudin1 in the colon, but hardly affected the expression of ZO\u20101 and Mucin2. In vitro, tetrandrine treatment rescued the decrease of monolayer transmembrane resistance and the increase of epithelial cell permeability induced by TNF\u2010\u03b1, upregulated the expression of Occludin, and downregulated the expression of Claudin1 but did not affect the expression of ZO\u20101. The siRNA of Occludin largely weakened the protective effect of tetrandrine on the epithelial barrier function in Caco\u20102 cells. MiR\u2010429 mimic obviously counteracted the upregulation of tetrandrine on the expression of Occludin and the amelioration on epithelial barrier defects, in contrast, miR\u2010429 inhibitor showed the opposite effects. The antagonist (CH223191) and siAhR of aryl hydrocarbon receptor (AhR) nearly completely diminished the effects of tetrandrine, including inhibition of the miR429 expression, the upregulation of Occludin expression, and amelioration of intestinal epithelial barrier defects in Caco\u20102 cells. In colitis mice, CH223191 significantly weakened the protective effect of tetrandrine on colitis and intestinal mucosal barrier and diminished the downregulation on miR\u2010429 expression and the promotion on Occludin expression in the colon. In summary, tetrandrine can attenuate the intestinal epithelial barrier defects in colitis through promoting Occludin expression via the AhR\/miR\u2010429 pathway, and it might be used to treat colitis as a barrier protector.\n                  <\/jats:p>","DOI":"10.1096\/fj.202002086rr","type":"journal-article","created":{"date-parts":[[2021,4,3]],"date-time":"2021-04-03T08:45:21Z","timestamp":1617439521000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":32,"title":["Tetrandrine attenuates intestinal epithelial barrier defects caused by colitis through promoting the expression of Occludin via the AhR\u2010miR\u2010429 pathway"],"prefix":"10.1096","volume":"35","author":[{"given":"Yuyao","family":"Chu","sequence":"first","affiliation":[{"name":"Department of Pharmacology of Chinese Materia Medica School of Traditional Chinese Pharmacy China Pharmaceutical University Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanrong","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Pharmacology of Chinese Materia Medica School of Traditional Chinese Pharmacy China Pharmaceutical University Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yajing","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Pharmacology of Chinese Materia Medica School of Traditional Chinese Pharmacy China Pharmaceutical University Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaojing","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Pharmacology of Chinese Materia Medica School of Traditional Chinese Pharmacy China Pharmaceutical University Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yilei","family":"Guo","sequence":"additional","affiliation":[{"name":"Department of Pharmacology of Chinese Materia Medica School of Traditional Chinese Pharmacy China Pharmaceutical University Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lan","family":"Chang","sequence":"additional","affiliation":[{"name":"Department of Pharmacology of Chinese Materia Medica School of Traditional Chinese Pharmacy China Pharmaceutical University Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinming","family":"Yun","sequence":"additional","affiliation":[{"name":"Department of Pharmacology of Chinese Materia Medica School of Traditional Chinese Pharmacy China Pharmaceutical University Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhifeng","family":"Wei","sequence":"additional","affiliation":[{"name":"Department of Pharmacology of Chinese Materia Medica School of Traditional Chinese Pharmacy China Pharmaceutical University Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yufeng","family":"Xia","sequence":"additional","affiliation":[{"name":"Department of Pharmacology of Chinese Materia Medica School of Traditional Chinese Pharmacy China Pharmaceutical University Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yue","family":"Dai","sequence":"additional","affiliation":[{"name":"Department of Pharmacology of Chinese Materia Medica School of Traditional Chinese Pharmacy China Pharmaceutical University Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,4,3]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrgastro.2016.169"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1159\/000358156"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1136\/gutjnl-2019-318427"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms20236020"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.4161\/tisb.28320"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnmol.2018.00035"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00441-016-2435-5"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1159\/000486059"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1701148"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bmcl.2019.06.013"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00384-008-0544-7"},{"key":"e_1_2_9_13_1","first-page":"56","article-title":"A preliminary experimental study on the effect of tetrandrine in reducing the early deepening burn wounds of second degree","volume":"30","author":"Zhang Y","year":"2017","journal-title":"Med Inform"},{"key":"e_1_2_9_14_1","first-page":"78","article-title":"Effects of pelliculea of tetrandrine et collagen compositus on wound healing after excision and combined radiation\u2010excision injury in rats","volume":"2","author":"Meng D","year":"1999","journal-title":"J Third Mil Med Univ"},{"key":"e_1_2_9_15_1","first-page":"1","article-title":"Protective effect of tetrandrine on sodium taurocholate\u2010induced severe acute pancreatitis","volume":"2015","author":"Wu X\u2010L","year":"2015","journal-title":"Evid Based Complement Alternat Med"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2018.07.039"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1080\/00365521.2019.1595708"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.201901638RR"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10753-019-01085-z"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0098016"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2011.07.005"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1186\/s11658-019-0146-0"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.29222"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12031-019-01400-1"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2015.11.025"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.201701148RR"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0954422416000019"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.2174\/1871527315666160915142244"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.5217\/ir.2015.13.1.11"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamem.2005.01.008"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.11.12.4131"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.e04-12-1089"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1080\/21688370.2017.1382671"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-018-05470-4"},{"key":"e_1_2_9_35_1","first-page":"1560","article-title":"Aryl hydrocarbon receptor activation maintained the intestinal epithelial barrier function through Notch1 dependent signaling pathway","volume":"41","author":"Liu Z","year":"2018","journal-title":"Int J Mol Med"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1158\/1535-7163.MCT-11-0548"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.taap.2014.08.023"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1093\/intimm\/dxv015"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep40539"}],"container-title":["The FASEB Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1096\/fj.202002086RR","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1096\/fj.202002086RR","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/faseb.onlinelibrary.wiley.com\/doi\/pdf\/10.1096\/fj.202002086RR","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T18:02:48Z","timestamp":1761674568000},"score":1,"resource":{"primary":{"URL":"https:\/\/faseb.onlinelibrary.wiley.com\/doi\/10.1096\/fj.202002086RR"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,4,3]]},"references-count":38,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2021,5]]}},"alternative-id":["10.1096\/fj.202002086RR"],"URL":"https:\/\/doi.org\/10.1096\/fj.202002086rr","archive":["Portico"],"relation":{},"ISSN":["0892-6638","1530-6860"],"issn-type":[{"value":"0892-6638","type":"print"},{"value":"1530-6860","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,4,3]]},"assertion":[{"value":"2020-09-07","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-02-19","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-04-03","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e21502"}}