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Mouse is the only mammalian model used for investigation of WAS pathogenesis. However, the mouse model does not accurately recapitulate WAS clinical phenotypes, thus, limiting its application in WAS clinical research. Herein, we report the generation of\n                    <jats:italic>WAS<\/jats:italic>\n                    knockout (KO) rabbits via embryo co\u2010injection of Cas9 mRNA and a pair of sgRNAs targeting exons 2 and 7.\n                    <jats:italic>WAS<\/jats:italic>\n                    KO rabbits exhibited many symptoms similar to those of WAS patients, including thrombocytopenia, bleeding tendency, infections, and reduced numbers of T cell in the spleen and peripheral blood. The\n                    <jats:italic>WAS<\/jats:italic>\n                    KO rabbit model provides a new valuable tool for preclinical trials of WAS treatment.\n                  <\/jats:p>","DOI":"10.1096\/fj.202002118rr","type":"journal-article","created":{"date-parts":[[2020,11,25]],"date-time":"2020-11-25T05:44:33Z","timestamp":1606283073000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Development of a rabbit model of Wiskott\u2010Aldrich syndrome"],"prefix":"10.1096","volume":"35","author":[{"given":"Juanjuan","family":"Zhou","sequence":"first","affiliation":[{"name":"School of Life Sciences University of Science and Technology of China Hefei China"},{"name":"CAS Key Laboratory of Regenerative Biology, Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health Chinese Academy of Sciences Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Quanmei","family":"Yan","sequence":"additional","affiliation":[{"name":"CAS Key Laboratory of Regenerative Biology, Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health Chinese Academy of Sciences Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chengcheng","family":"Tang","sequence":"additional","affiliation":[{"name":"School of Biotechnology and Health Sciences Wuyi University Jiangmen China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuan","family":"Liao","sequence":"additional","affiliation":[{"name":"CAS Key Laboratory of Regenerative Biology, Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health Chinese Academy of Sciences Guangzhou China"},{"name":"Institute of Physical Science and Information Technology Anhui University Hefei China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Quanjun","family":"Zhang","sequence":"additional","affiliation":[{"name":"CAS Key Laboratory of Regenerative Biology, Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health Chinese Academy of Sciences Guangzhou China"},{"name":"Bioland Laboratory (Guangzhou Regenerative Medicine and Health Guangdong Laboratory) Guangzhou China"},{"name":"Institute for Stem Cell and Regeneration Chinese Academy of Sciences Beijing China"},{"name":"Research Unit of Generation of Large Animal Disease Models Chinese Academy of Medical Sciences (2019RU015) Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaomin","family":"Wang","sequence":"additional","affiliation":[{"name":"CAS Key Laboratory of Regenerative Biology, Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health Chinese Academy of Sciences Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoqing","family":"Zhou","sequence":"additional","affiliation":[{"name":"School of Biotechnology and Health Sciences Wuyi University Jiangmen China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liangxue","family":"Lai","sequence":"additional","affiliation":[{"name":"CAS Key Laboratory of Regenerative Biology, Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health Chinese Academy of Sciences Guangzhou China"},{"name":"Bioland Laboratory (Guangzhou Regenerative Medicine and Health Guangdong Laboratory) Guangzhou China"},{"name":"Institute for Stem Cell and Regeneration Chinese Academy of Sciences Beijing China"},{"name":"Research Unit of Generation of Large Animal Disease Models Chinese Academy of Medical Sciences (2019RU015) Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qingjian","family":"Zou","sequence":"additional","affiliation":[{"name":"School of Biotechnology and Health Sciences Wuyi University Jiangmen 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