{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T19:28:31Z","timestamp":1791919711189,"version":"4.3.4"},"reference-count":51,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2021,4,27]],"date-time":"2021-04-27T00:00:00Z","timestamp":1619481600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81573310"],"award-info":[{"award-number":["81573310"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000054","name":"National Cancer Institute","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000054","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2021,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Saururus chinensis<\/jats:italic>\n                    (\n                    <jats:italic>S<\/jats:italic>\n                    \u00a0\n                    <jats:italic>chinensis<\/jats:italic>\n                    ) has been used as an herb to treat edema, jaundice, and gonorrhea. Manassantin B (MNSB), a dineolignan isolated from\n                    <jats:italic>S\u00a0chinensis<\/jats:italic>\n                    , was identified as a potent adipogenesis\/lipogenesis inhibitor (IC\n                    <jats:sub>50<\/jats:sub>\n                    \u00a0=\u00a09.3\u00a0nM). To explore the underlying mechanism, both adipogenesis and lipogenesis were measured in differentiated 3T3\u2010L1 preadipocytes, murine primary preadipocytes and adipose tissue explants upon MNSB treatment. Key regulators of adipogenesis\/lipogenesis were downregulated by MNSB treatment, mainly resulting from increased phosphorylation of AMPK which was identified as a vital regulator of adipogenesis and lipogenesis. Moreover, MNSB did not increase AMPK phosphorylation in 3T3\u2010L1 cells transfected with\n                    <jats:italic>Prkaa1<\/jats:italic>\n                    (encoding protein kinase AMP\u2010activated catalytic subunit alpha 1) siRNA or adipose tissue explants isolated from adipose\u2010specific\n                    <jats:italic>Prkaa1<\/jats:italic>\n                    \u2010disrupted mice (\n                    <jats:italic>Prkaa1<\/jats:italic>\n                    <jats:sup>\u0394ad<\/jats:sup>\n                    ). In diet\u2010induced obese C57BL\/6N mice, MNSB displayed preventive and therapeutic effects on obesity accompanied by decreased adipocyte size. MNSB was also found to increase AMPK phosphorylation both in subcutaneous white adipose tissue and brown adipose tissue in vivo. These findings suggest that MNSB can be a new therapeutic agent for the prevention and treatment of obesity and other related metabolic disorders.\n                  <\/jats:p>","DOI":"10.1096\/fj.202002126rr","type":"journal-article","created":{"date-parts":[[2021,4,27]],"date-time":"2021-04-27T15:14:04Z","timestamp":1619536444000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Manassantin B attenuates obesity by inhibiting adipogenesis and lipogenesis in an AMPK dependent manner"],"prefix":"10.1096","volume":"35","author":[{"given":"Jie","family":"Cai","sequence":"first","affiliation":[{"name":"Research Center for Drug Discovery School of Pharmaceutical Sciences Sun Yat\u2010Sen University Guangzhou China"},{"name":"Laboratory of Metabolism Center for Cancer Research National Cancer Institute Bethesda MD 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