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Fluorofenidone (AKF\u2010PD) is a novel pyridone agent able to ameliorate hepatic fibrosis in an experimental hepatic fibrosis model induced by dimethylnitrosamine. However, the underlying mechanism remains to be further elucidated. In light of the critical role of the NF\u2010\u03baB pathway in inflammation and hepatic fibrosis, together with the preliminary finding that AKF\u2010PD decreases the release of proinflammatory cytokines in the endotoxemia and unilateral ureteral occlusion model, the aim of this study was to explore whether AKF\u2010PD exerts an antifibrotic effect in hepatic fibrosis by inhibiting inflammation and suppressing the activation of the NF\u2010\u03baB pathway in vivo and in vitro. To test this possibility, the effect of AKF\u2010PD on hepatic fibrosis models induced by both carbon tetrachloride (CCL\n                    <jats:sub>4<\/jats:sub>\n                    ) and porcine serum (PS) was investigated. Our results showed that AKF\u2010PD treatment ameliorated hepatic injury and fibrosis in both models. Furthermore, the administration of AKF\u2010PD induced a robust anti\u2010inflammatory reaction revealed by the downregulation of the proinflammatory cytokines as well as the suppression of the infiltration of inflammatory cells in the fibrotic liver. The analysis of the mechanism of action demonstrated that the attenuation of the production of proinflammatory cytokines and chemokines mediated by AKF\u2010PD in vivo and in vitro were accompanied by the suppression in the activation of the NF\u2010\u03baB signaling pathway. In conclusion, AKF\u2010PD might be considered as an antifibrotic agent attenuating hepatic inflammation and fibrosis potentially through the suppression of the NF\u2010\u03baB pathway.\n                  <\/jats:p>","DOI":"10.1096\/fj.202002402r","type":"journal-article","created":{"date-parts":[[2021,6,21]],"date-time":"2021-06-21T06:44:33Z","timestamp":1624257873000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Fluorofenidone protects liver against inflammation and fibrosis by blocking the activation of NF\u2010\u03baB pathway"],"prefix":"10.1096","volume":"35","author":[{"given":"Sha","family":"Tu","sequence":"first","affiliation":[{"name":"Department of Gastroenterology Xiangya Hospital Central South University Changsha P.R. China"},{"name":"Hunan Key Laboratory of Organ Fibrosis Central South University Changsha P.R. 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