{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,1]],"date-time":"2025-12-01T06:35:41Z","timestamp":1764570941551,"version":"3.46.0"},"reference-count":20,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2021,5,12]],"date-time":"2021-05-12T00:00:00Z","timestamp":1620777600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["18K14603","20H05678"],"award-info":[{"award-number":["18K14603","20H05678"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007449","name":"Takeda Science Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007449","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2021,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    IgE\u2010dependent\/independent activation of mast cell (MC) has been assumed to play a host defensive role against venom injection in skin. However, its detailed mechanisms remain unknown. We aimed to investigate the contribution of MC\u2010derived prostaglandin D\n                    <jats:sub>2<\/jats:sub>\n                    (PGD\n                    <jats:sub>2<\/jats:sub>\n                    )\u2010mediated signaling in host defense against bee venom (BV). To achieve this, we utilized gene\u2010deficient mice of a PGD\n                    <jats:sub>2<\/jats:sub>\n                    receptor, chemoattractant receptor\u2010homologous molecule expressed on Th2 cells (CRTH2). We first confirmed that subcutaneous injection of BV produced PGD\n                    <jats:sub>2<\/jats:sub>\n                    equally in wild\u2010type (WT) and CRTH2\u2010deficient (\n                    <jats:italic>\n                      Crth2\n                      <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    <\/jats:italic>\n                    ) mice skins. The BV injection dropped body temperature and impaired kidney equally in both lines of mice. In WT mice, pre\u2010injection of BV (3\u00a0weeks) significantly inhibited the hypothermia and kidney impairment caused by second BV injection. In contrast, this pre\u2010injection was not effective for the second BV injection in\n                    <jats:italic>\n                      Crth2\n                      <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    <\/jats:italic>\n                    mice. We also found that BV injections increased serum BV\u2010specific IgE levels in WT mice, and its serum transfused mice improved the BV\u2010induced hypothermia in na\u00efve WT mice. In contrast, serum BV\u2010specific IgE level was significantly lower in\n                    <jats:italic>\n                      Crth2\n                      <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    <\/jats:italic>\n                    mice. FACS analysis showed the BV injection stimulate migration of dendritic cells (DCs) into regional lymph nodes in WT mice. In\n                    <jats:italic>\n                      Crth2\n                      <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    <\/jats:italic>\n                    mice, its number was significantly smaller than that of WT mice. In conclusion, PGD\n                    <jats:sub>2<\/jats:sub>\n                    \/CRTH2 signaling plays defensive role against second BV injection. This signaling promotes BV\u2010specific IgE production at least partially by promoting DCs migration into regional lymph node.\n                  <\/jats:p>","DOI":"10.1096\/fj.202002748rr","type":"journal-article","created":{"date-parts":[[2021,5,12]],"date-time":"2021-05-12T08:52:41Z","timestamp":1620809561000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["PGD\n                    <sub>2<\/sub>\n                    \/CRTH2 signaling promotes acquired immunity against bee venom by enhancing IgE production"],"prefix":"10.1096","volume":"35","author":[{"given":"Misato","family":"Kida","sequence":"first","affiliation":[{"name":"Animal Radiology Graduate School of Agricultural and Life Sciences The University of Tokyo Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tatsuro","family":"Nakamura","sequence":"additional","affiliation":[{"name":"Animal Radiology Graduate School of Agricultural and Life Sciences The University of Tokyo Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuki","family":"Fujiwara","sequence":"additional","affiliation":[{"name":"Animal Radiology Graduate School of Agricultural and Life Sciences The University of Tokyo Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masataka","family":"Nakamura","sequence":"additional","affiliation":[{"name":"Human Gene Sciences Center Tokyo Medical and Dental University Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3328-0796","authenticated-orcid":false,"given":"Takahisa","family":"Murata","sequence":"additional","affiliation":[{"name":"Animal Radiology Graduate School of Agricultural and Life Sciences The University of Tokyo Tokyo 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