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Therefore, there is an unmet need globally for lifestyle interventions to reduce the likelihood of bone fractures with age. Although many mechanisms are involved in disorders of bone loss, there is no single regulatory pathway and, therefore, there is no single treatment available to prevent their occurrence. Our aim in these studies was to determine whether fasting\/feeding interventions alter the effect of mechanical loading on bone anabolic activities and increase bone mass. In young 17\u2010week\u2010old mice, 16\u2010hour fasting period followed by reintroduction of food for 2\u00a0hours increased markedly the potency of mechanical loading, that mimics the effect of exercise, to induce new cortical bone formation. Consistent with this finding, fasting and re\u2010feeding increased the response of bone to a loading stimulus that, alone, does not stimulate new bone formation in ad\u2010lib fed mice. Older mice (20\u00a0months) experienced no potentiation of loading\u2010induced bone formation with the same timing of feeding interventions. Interestingly, the pre\u2010, prandial, and postprandial endocrine responses in older mice were different from those in young animals. The hormones that change in response to timing of feeding have osteogenic effects that interact with loading\u2010mediated effects. Our findings indicate associations between timing of food ingestion and bone adaptation to loading. If translated to humans, such non\u2010pharmacological lifestyle interventions may benefit skeletal health of humans throughout life\u2010course and in older age.<\/jats:p>","DOI":"10.1096\/fj.202100334rr","type":"journal-article","created":{"date-parts":[[2021,9,13]],"date-time":"2021-09-13T13:20:30Z","timestamp":1631539230000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Feeding intervention potentiates the effect of mechanical loading to induce new bone formation in mice"],"prefix":"10.1096","volume":"35","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9576-8001","authenticated-orcid":false,"given":"Hasmik Jasmine","family":"Samvelyan","sequence":"first","affiliation":[{"name":"CIMA, MRC\u2010Versus Arthritis Centre for Integrated Research into Musculoskeletal Ageing The University of Sheffield Sheffield UK"},{"name":"Department of Oncology and Metabolism The Medical School Mellanby Centre for Bone 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