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A short, OHC\u2010specific enhancer is necessary but not yet available for gene therapeutic applications in OHC damage. Such damage is a major cause of deafness. Prestin is a motor protein exclusively expressed in OHCs. We hypothesized that the\n                    <jats:italic>cis<\/jats:italic>\n                    \u2010regulatory DNA fragment deletion of\n                    <jats:italic>Slc26a5<\/jats:italic>\n                    would affect its expression. We tested this hypothesis by conducting CRISPR\/Cas9\u2010mediated large DNA fragment deletion of mouse\n                    <jats:italic>Slc26a5<\/jats:italic>\n                    intron regions. First, starting from a ~13\u00a0kbp fragment, step\u2010by\u2010step, we narrowed down the sequence to a 1.4\u00a0kbp segment. By deleting either a 13\u00a0kbp or 1.4\u00a0kbp fragment, we observed delayed Prestin expression. Second, we showed that 1.4\u00a0kbp was an OHC\u2010specific enhancer because enhanced green fluorescent protein (EGFP) was highly and specifically expressed in OHCs in a transgenic mouse where EGFP was driven by the 1.4\u00a0kbp segment. More importantly, specific EGFP was also driven by its homologous 398\u00a0bp fragment in human\n                    <jats:italic>Slc26a5<\/jats:italic>\n                    . This suggests that the enhancer is likely to be evolutionarily conserved across different species.\n                  <\/jats:p>","DOI":"10.1096\/fj.202100421rr","type":"journal-article","created":{"date-parts":[[2022,2,28]],"date-time":"2022-02-28T03:40:54Z","timestamp":1646019654000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["In vivo CRISPR\u2010Cas9\u2010mediated DNA chop identifies a cochlear outer hair cell\u2010specific enhancer"],"prefix":"10.1096","volume":"36","author":[{"given":"Yuwei","family":"Sun","sequence":"first","affiliation":[{"name":"Institute of Neuroscience State Key Laboratory of Neuroscience CAS Center for Excellence in Brain Science and Intelligence Technology Chinese\u00a0Academy of Sciences  Shanghai China"},{"name":"University of Chinese Academy of Sciences  Beijing 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