{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,28]],"date-time":"2025-11-28T18:54:59Z","timestamp":1764356099425,"version":"3.46.0"},"reference-count":56,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2021,5,27]],"date-time":"2021-05-27T00:00:00Z","timestamp":1622073600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["AI077538"],"award-info":[{"award-number":["AI077538"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2021,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    The bloodstream stage of\n                    <jats:italic>Trypanosoma brucei<\/jats:italic>\n                    , the causative agent of African trypanosomiasis, is characterized by its high rate of endocytosis, which is involved in remodeling of its surface coat. Here we present evidence that RNAi\u2010mediated expression down\u2010regulation of vacuolar protein sorting 41 (Vps41), a component of the homotypic fusion and vacuole protein sorting (HOPS) complex, leads to a strong inhibition of endocytosis, vesicle accumulation, enlargement of the flagellar pocket (\u201cbig eye\u201d phenotype), and dramatic effect on cell growth. Unexpectedly, other functions described for Vps41 in mammalian cells and yeasts, such as delivery of proteins to lysosomes, and lysosome\u2010related organelles (acidocalcisomes) were unaffected, indicating that in trypanosomes post\u2010Golgi trafficking is distinct from that of mammalian cells and yeasts. The essentiality of TbVps41 suggests that it is a potential drug target.\n                  <\/jats:p>","DOI":"10.1096\/fj.202100487r","type":"journal-article","created":{"date-parts":[[2021,5,27]],"date-time":"2021-05-27T03:43:36Z","timestamp":1622087016000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["TbVps41 regulates trafficking of endocytic but not biosynthetic cargo to lysosomes of bloodstream forms of\n                    <i>Trypanosoma brucei<\/i>"],"prefix":"10.1096","volume":"35","author":[{"given":"Srinivasan","family":"Ramakrishnan","sequence":"first","affiliation":[{"name":"Center for Tropical and Emerging Global Diseases Athens GA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rodrigo P.","family":"Baptista","sequence":"additional","affiliation":[{"name":"Center for Tropical and Emerging Global Diseases Athens GA 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