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The serum level of thymus and activation\u2010regulated chemokine (TARC) is a useful AD index to reflect disease severity; however, it requires blood collection from young children. In comparison, urine samples are easier to collect in a pediatric clinical setting. Here, we analyzed the lipids excreted in urine to identify a diagnostic biomarker for AD. We generated a murine dermatitis model by repeated topical application of 2,4\u2010dinitrofluorobenzene (DNFB) or tape\u2010stripping the dorsal skin. Lipid metabolites excreted in the urine were comprehensively analyzed using liquid chromatography\u2010tandem mass spectrometry. To corroborate our findings, we also analyzed urine samples from patients with AD. DNFB application induced AD\u2010like skin lesions, including epidermal thickening, infiltration of eosinophils and T cells, and an increase in Th2 cytokine levels. Assessment of lipids excreted in urine showed a dominance of prostaglandins (PGs), namely, a PGF\n                    <jats:sub>2\u03b1<\/jats:sub>\n                    metabolite (13,14\u2010dihydro\u201015\u2010keto\u2010tetranor\u2010PGF\n                    <jats:sub>1\u03b1<\/jats:sub>\n                    ), a PGE\n                    <jats:sub>2<\/jats:sub>\n                    metabolite (13,14\u2010dihydro\u201015\u2010keto\u2010tetranor\u2010PGE\n                    <jats:sub>2<\/jats:sub>\n                    ), and a PGD\n                    <jats:sub>2<\/jats:sub>\n                    metabolite (13,14\u2010dihydro\u201015\u2010keto PGJ\n                    <jats:sub>2<\/jats:sub>\n                    ). mRNA and protein expression of PGF\n                    <jats:sub>2\u03b1<\/jats:sub>\n                    , PGE\n                    <jats:sub>2<\/jats:sub>\n                    , and PGD\n                    <jats:sub>2<\/jats:sub>\n                    synthase was upregulated in DNFB\u2010treated skin. The tape\u2010stripping model also caused dermatitis but without Th2 inflammation; urine PGF\n                    <jats:sub>2\u03b1<\/jats:sub>\n                    and PGD\n                    <jats:sub>2<\/jats:sub>\n                    metabolite levels remained unaffected. Finally, we confirmed that the urinary levels of the aforementioned PG metabolites, as well as PGI\n                    <jats:sub>2<\/jats:sub>\n                    metabolite, 6,15\u2010diketo\u201013,14\u2010dihydro\u2010PGF\n                    <jats:sub>1\u03b1<\/jats:sub>\n                    and arachidonic acid metabolite, 17\u2010hydroxyeicosatetraenoic acid (17\u2010HETE) increased in patients with AD. Our data highlights the unique urinary lipid profile in patients with AD, which may provide insight into novel urinary biomarkers for AD diagnosis.\n                  <\/jats:p>","DOI":"10.1096\/fj.202100828r","type":"journal-article","created":{"date-parts":[[2021,9,30]],"date-time":"2021-09-30T15:13:43Z","timestamp":1633014823000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Urinary lipid profile of atopic dermatitis in murine model and human patients"],"prefix":"10.1096","volume":"35","author":[{"given":"Nanae","family":"Nagata","sequence":"first","affiliation":[{"name":"Department of Animal Radiology Graduate School of Agricultural and Life Sciences The University of Tokyo Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuta","family":"Hamasaki","sequence":"additional","affiliation":[{"name":"Department of Animal Radiology Graduate School of Agricultural and Life Sciences The University of Tokyo Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shinichiro","family":"Inagaki","sequence":"additional","affiliation":[{"name":"Allergy Center National Center for Child Health and Development Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tatsuro","family":"Nakamura","sequence":"additional","affiliation":[{"name":"Department of Animal Radiology Graduate School of Agricultural and Life Sciences The University of Tokyo Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daiki","family":"Horikami","sequence":"additional","affiliation":[{"name":"Department of Animal Radiology Graduate School of Agricultural and Life Sciences The University of Tokyo Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1288-9620","authenticated-orcid":false,"given":"Kiwako","family":"Yamamoto\u2010Hanada","sequence":"additional","affiliation":[{"name":"Allergy Center National Center for Child Health and Development Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yusuke","family":"Inuzuka","sequence":"additional","affiliation":[{"name":"Allergy Center National Center for Child Health and Development Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tatsuo","family":"Shimosawa","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory School of Medicine International University of Health and Welfare Chiba Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Koji","family":"Kobayashi","sequence":"additional","affiliation":[{"name":"Department of Animal Radiology Graduate School of Agricultural and Life 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