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Our data indicate that Gal\u20109 is upregulated in the inflamed vasculature of RA synovial biopsies and report the release of Gal\u20109 into the extracellular environment following endothelial cell activation. siRNA knockdown of endothelial Gal\u20109 resulted in reduced neutrophil adhesion and neutrophil recruitment was significantly reduced in Gal\u20109\u00a0knockout mice in a model of zymosan\u2010induced peritonitis. We also provide evidence for Gal\u20109 binding sites on human neutrophils; Gal\u20109 binding induced neutrophil activation (increased expression of \u03b22 integrins and reduced expression of CD62L). Intra\u2010vital microscopy confirmed a pro\u2010recruitment role for Gal\u20109, with increased numbers of transmigrated neutrophils following Gal\u20109 administration. We studied the role of both soluble and immobilized Gal\u20109 on human neutrophil recruitment. Soluble Gal\u20109\u00a0significantly strengthened the interaction between neutrophils and the endothelium and inhibited neutrophil crawling on ICAM\u20101. When immobilized, Gal\u20109 functioned as an adhesion molecule and captured neutrophils from the flow. Neutrophils adherent to Gal\u20109 exhibited a spread\/activated phenotype that was inhibited by CD18 and CD44 neutralizing antibodies, suggesting a role for these molecules in the pro\u2010adhesive effects of Gal\u20109. Our data indicate that Gal\u20109 is expressed and released by the activated endothelium and functions both in soluble form and when immobilized as a neutrophil adhesion molecule. This study paves the way for further investigation of the role of Gal\u20109 in leukocyte recruitment in different inflammatory settings.<\/jats:p>","DOI":"10.1096\/fj.202100832r","type":"journal-article","created":{"date-parts":[[2021,11,30]],"date-time":"2021-11-30T18:33:15Z","timestamp":1638297195000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":46,"title":["Galectin\u20109 mediates neutrophil capture and adhesion in a CD44 and \u03b22 integrin\u2010dependent manner"],"prefix":"10.1096","volume":"36","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3224-3651","authenticated-orcid":false,"given":"Asif J.","family":"Iqbal","sequence":"first","affiliation":[{"name":"Institute of Cardiovascular Sciences (ICVS) College of Medical and Dental Sciences University of Birmingham  Birmingham UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Franziska","family":"Krautter","sequence":"additional","affiliation":[{"name":"Institute of Cardiovascular Sciences (ICVS) College of Medical and Dental Sciences University of Birmingham  Birmingham UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isobel A.","family":"Blacksell","sequence":"additional","affiliation":[{"name":"The William Harvey Research Institute Barts and The London School of Medicine Queen Mary University of London  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rachael D.","family":"Wright","sequence":"additional","affiliation":[{"name":"The William Harvey Research Institute Barts and The London School of Medicine Queen Mary University of London  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shani N.","family":"Austin\u2010Williams","sequence":"additional","affiliation":[{"name":"The William Harvey Research Institute Barts and The London School of Medicine Queen Mary University of London  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mathieu\u2010Benoit","family":"Voisin","sequence":"additional","affiliation":[{"name":"The William Harvey Research Institute Barts and The London School of Medicine Queen Mary University of London  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammed T.","family":"Hussain","sequence":"additional","affiliation":[{"name":"Institute of Cardiovascular Sciences (ICVS) College of Medical and Dental Sciences University of Birmingham  Birmingham UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hannah L.","family":"Law","sequence":"additional","affiliation":[{"name":"The William Harvey Research Institute Barts and The London School of Medicine Queen Mary University of London  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Toshiro","family":"Niki","sequence":"additional","affiliation":[{"name":"Research Division GalPharma Company, Ltd.  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