{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T21:29:10Z","timestamp":1788989350766,"version":"build-2803163510"},"reference-count":64,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2021,11,25]],"date-time":"2021-11-25T00:00:00Z","timestamp":1637798400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["NIH R01 AR073225"],"award-info":[{"award-number":["NIH R01 AR073225"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004543","name":"China Scholarship Council","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004543","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100006518","name":"University of Nebraska Medical Center","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100006518","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2021,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Rotator cuff tendon injuries often occur at the tendon\u2010to\u2010bone interface (i.e., enthesis) area, with a high prevalence for the elderly population, but the underlying reason for this phenomenon is still unknown. The objective of this study is to identify the histological, molecular, and biomechanical alterations of the rotator cuff enthesis with maturation and aging in a mouse model. Four different age groups of mice (newborn, young, adult, and old) were studied. Striking variations of the entheses were observed between the newborn and other matured groups, with collagen content, proteoglycan deposition, collagen fiber dispersion was significantly higher in the newborn group. The compositional and histological features of young, adult, and old groups did not show significant differences, except having increased proteoglycan deposition and thinner collagen fibers at the insertion sites in the old group. Nanoindentation testing showed that the old group had a smaller compressive modulus at the insertion site when compared with other groups. However, tensile mechanical testing reported that the old group demonstrated a significantly higher failure stress when compared with the young and adult groups. The proteomics analysis detected dramatic differences in protein content between newborn and young groups but minor changes among young, adult, and old groups. These results demonstrated: (1) the significant alterations of the enthesis composition and structure occur from the newborn to the young time period; (2) the increased risk of rotator cuff tendon injuries in the elderly population is not solely because of old age alone in the rodent model.<\/jats:p>","DOI":"10.1096\/fj.202101484r","type":"journal-article","created":{"date-parts":[[2021,11,26]],"date-time":"2021-11-26T05:43:28Z","timestamp":1637905408000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["The effects of maturation and aging on the rotator cuff tendon\u2010to\u2010bone interface"],"prefix":"10.1096","volume":"35","author":[{"given":"Xiping","family":"Jiang","sequence":"first","affiliation":[{"name":"Mary &amp; Dick Holland Regenerative Medicine Program University of Nebraska Medical Center Omaha Nebraska USA"},{"name":"Division of Cardiology Department of Internal Medicine University of Nebraska Medical Center Omaha Nebraska USA"},{"name":"Molecular Genetics and Cell Biology Program Department of Genetics, Cell Biology and Anatomy University of Nebraska Medical Center Omaha Nebraska USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Melinda","family":"Wojtkiewicz","sequence":"additional","affiliation":[{"name":"CardiOmics Program, Center for Heart and Vascular Research Division of Cardiovascular Medicine, and Department of Cellular and Integrative Physiology University of Nebraska Medical Center Omaha Nebraska USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chinmay","family":"Patwardhan","sequence":"additional","affiliation":[{"name":"Department of Bioengineering University of Texas at Arlington Arlington Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sydney","family":"Greer","sequence":"additional","affiliation":[{"name":"Mary &amp; Dick Holland Regenerative Medicine Program University of Nebraska Medical Center Omaha Nebraska USA"},{"name":"Molecular Genetics and Cell Biology Program Department of Genetics, Cell Biology and Anatomy University of Nebraska Medical Center Omaha Nebraska USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yunfan","family":"Kong","sequence":"additional","affiliation":[{"name":"Mary &amp; Dick Holland Regenerative Medicine Program University of Nebraska Medical Center Omaha Nebraska USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mitchell","family":"Kuss","sequence":"additional","affiliation":[{"name":"Mary &amp; Dick Holland Regenerative Medicine Program University of Nebraska Medical Center Omaha Nebraska USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xi","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Electrical and Computer Engineering University of Nebraska\u2010Lincoln Lincoln Nebraska USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Liao","sequence":"additional","affiliation":[{"name":"Department of Bioengineering University of Texas at Arlington Arlington Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongfeng","family":"Lu","sequence":"additional","affiliation":[{"name":"Department of Electrical and Computer Engineering University of Nebraska\u2010Lincoln Lincoln Nebraska USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew","family":"Dudley","sequence":"additional","affiliation":[{"name":"Mary &amp; Dick Holland Regenerative Medicine Program University of Nebraska Medical Center Omaha Nebraska USA"},{"name":"Molecular Genetics and Cell Biology Program Department of Genetics, Cell Biology and Anatomy University of Nebraska Medical Center Omaha Nebraska USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rebekah L.","family":"Gundry","sequence":"additional","affiliation":[{"name":"CardiOmics Program, Center for Heart and Vascular Research Division of Cardiovascular Medicine, and Department of Cellular and Integrative Physiology University of Nebraska Medical Center Omaha Nebraska USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthias","family":"Fuchs","sequence":"additional","affiliation":[{"name":"Department of Physics and Astronomy University of Nebraska\u2010Lincoln Lincoln Nebraska USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philipp","family":"Streubel","sequence":"additional","affiliation":[{"name":"Department of Orthopedic Surgery and Rehabilitation University of Nebraska Medical Center Omaha Nebraska USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5647-3793","authenticated-orcid":false,"given":"Bin","family":"Duan","sequence":"additional","affiliation":[{"name":"Mary &amp; Dick Holland Regenerative Medicine Program University of Nebraska Medical Center Omaha Nebraska USA"},{"name":"Division of Cardiology Department of Internal Medicine University of Nebraska Medical Center Omaha Nebraska USA"},{"name":"Department of Surgery College of Medicine University of Nebraska Medical Center Omaha Nebraska USA"},{"name":"Department of Mechanical and Materials Engineering University of Nebraska\u2010Lincoln Lincoln Nebraska USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,11,25]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.2106\/JBJS.E.00835"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1097\/BLO.0b013e31802fc175"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1136\/ard.54.12.959"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1302\/0301-620X.77B2.7706351"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jse.2014.08.001"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.2106\/JBJS.16.00770"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1177\/0363546517695234"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2019.05.041"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.32098\/mltj.03.2014.12"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.3109\/03008207.2011.650804"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1186\/1749-799X-5-59"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-bioeng-071910-124656"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1095-6433(02)00138-1"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.csm.2008.09.002"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.addr.2015.03.004"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00198-009-1066-z"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.matbio.2013.01.003"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1196\/annals.1333.082"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.093906"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.096354"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/jor.20418"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/jor.22151"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bone.2013.03.010"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1177\/0363546513518418"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/jor.20149"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0003-9969(01)00072-3"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.2527\/jas1962.21133x"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuint.2018.10.005"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.274.5289.998"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2006.85"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.14-258236"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.112714"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-62703-989-5_11"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.21769\/BioProtoc.3629"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.arthro.2016.04.019"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.2147\/CCID.S143015"},{"key":"e_1_2_9_38_1","first-page":"97","article-title":"Collagen and picrosirius red staining: a polarized light assessment of fibrillar hue and spatial distribution","volume":"22","author":"Rich L","year":"2017","journal-title":"J Morphol Sci"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1109\/ICIG.2013.197"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-019-13993-7"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmbbm.2020.104031"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2016.03.007"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.tec.2014.0589"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2016.12.036"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2013.01.031"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2018.08.014"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymeth.2020.09.012"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.1177\/089686080502500603"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms18122557"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmbbm.2008.10.005"},{"key":"e_1_2_9_51_1","doi-asserted-by":"publisher","DOI":"10.1097\/BCO.0b013e3282630851"},{"key":"e_1_2_9_52_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10856-011-4320-9"},{"key":"e_1_2_9_53_1","doi-asserted-by":"publisher","DOI":"10.1002\/term.2258"},{"key":"e_1_2_9_54_1","doi-asserted-by":"publisher","DOI":"10.1097\/GME.0b013e31829638e3"},{"key":"e_1_2_9_55_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.otsr.2017.03.027"},{"key":"e_1_2_9_56_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0945-053X(98)00006-7"},{"key":"e_1_2_9_57_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biocel.2010.12.010"},{"key":"e_1_2_9_58_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm3902"},{"key":"e_1_2_9_59_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.108.1.191"},{"key":"e_1_2_9_60_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.matbio.2020.09.001"},{"key":"e_1_2_9_61_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.matbio.2017.02.002"},{"key":"e_1_2_9_62_1","doi-asserted-by":"publisher","DOI":"10.1098\/rstb.2001.1029"},{"key":"e_1_2_9_63_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0891-5849(02)00905-X"},{"key":"e_1_2_9_64_1","doi-asserted-by":"publisher","DOI":"10.3109\/10409238.2013.770819"},{"key":"e_1_2_9_65_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-3233(05)70013-9"}],"container-title":["The FASEB Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1096\/fj.202101484R","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1096\/fj.202101484R","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/faseb.onlinelibrary.wiley.com\/doi\/pdf\/10.1096\/fj.202101484R","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T22:50:01Z","timestamp":1761691801000},"score":1,"resource":{"primary":{"URL":"https:\/\/faseb.onlinelibrary.wiley.com\/doi\/10.1096\/fj.202101484R"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,11,25]]},"references-count":64,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2021,12]]}},"alternative-id":["10.1096\/fj.202101484R"],"URL":"https:\/\/doi.org\/10.1096\/fj.202101484r","archive":["Portico"],"relation":{},"ISSN":["0892-6638","1530-6860"],"issn-type":[{"value":"0892-6638","type":"print"},{"value":"1530-6860","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,11,25]]},"assertion":[{"value":"2021-09-17","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-11-10","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-11-25","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e22066"}}