{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,18]],"date-time":"2026-06-18T16:52:06Z","timestamp":1781801526464,"version":"3.54.5"},"reference-count":59,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2022,2,24]],"date-time":"2022-02-24T00:00:00Z","timestamp":1645660800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2022,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Infertility is a social and medical problem around the world and the incidence continues to rise. Thin endometrium (TE) is a great challenge of infertility treatment, even by in vitro fertilization and embryo transfer. It is widely believed that TE impairs endometrium receptivity. However, only a few studies have explained the molecular mechanism. Herein, in order to reveal the possible mechanism, we sampled endometrium from a TE patient and a control volunteer and got a transcriptomic atlas of 18\u00a0775 individual cells which was constructed using single\u2010cell RNA sequencing, and seven cell types have been identified. The cells were acquired during proliferative and secretory phases, respectively. The proportion of epithelial cells and stromal cells showed a significant difference between the TE group and the control group. In addition, differential expressed genes (DEGs) in diverse cell types were revealed, the enriched pathways of DEGs were found closely related to the protein synthesis in TE of both proliferative and secretory phases. Some DEGs can influence cell\u2010type ratio and impaired endometrial receptivity in TE. Furthermore, divergent expression of estrogen receptors 1 and progesterone receptors in stromal and epithelial cells were compared in the TE sample from the control. The cellular and molecular heterogeneity found in this study provided valuable information for disclosing the mechanisms of impaired receptivity in TE.<\/jats:p>","DOI":"10.1096\/fj.202101579r","type":"journal-article","created":{"date-parts":[[2022,2,24]],"date-time":"2022-02-24T15:17:22Z","timestamp":1645715842000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Single\u2010cell transcriptome analysis uncovers the molecular and cellular characteristics of thin endometrium"],"prefix":"10.1096","volume":"36","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9461-9191","authenticated-orcid":false,"given":"Xirong","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynecology Qingdao Medical College of Qingdao University  Qingdao China"},{"name":"Center for Reproductive Medicine, Department of Reproductive Endocrinology Zhejiang Provincial People\u2019s Hospital (Affiliated People\u2019s Hospital, Hangzhou Medical College)  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yini","family":"Li","sequence":"additional","affiliation":[{"name":"School of Life Sciences Westlake University  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaopan","family":"Chen","sequence":"additional","affiliation":[{"name":"Center for Reproductive Medicine, Department of Reproductive Endocrinology Zhejiang Provincial People\u2019s Hospital (Affiliated People\u2019s Hospital, Hangzhou Medical College)  Hangzhou China"},{"name":"Department of Genetic and Genomic Medicine Zhejiang Provincial People\u2019s Hospital, (Affiliated People\u2019s Hospital, Hangzhou Medical College)  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bihui","family":"Jin","sequence":"additional","affiliation":[{"name":"Center for Reproductive Medicine, Department of Reproductive Endocrinology Zhejiang Provincial People\u2019s Hospital (Affiliated People\u2019s Hospital, Hangzhou Medical College)  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chongyi","family":"Shu","sequence":"additional","affiliation":[{"name":"Center for Reproductive Medicine, Department of Reproductive Endocrinology Zhejiang Provincial People\u2019s Hospital (Affiliated People\u2019s Hospital, Hangzhou Medical College)  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wanmao","family":"Ni","sequence":"additional","affiliation":[{"name":"Cancer Center, Key Laboratory of Tumor Molecular Diagnosis and Individualized Medicine of Zhejiang Province Zhejiang Provincial People\u2019s Hospital (Affiliated People\u2019s Hospital, Hangzhou Medical College)  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yinshen","family":"Jiang","sequence":"additional","affiliation":[{"name":"Center for Reproductive Medicine, Department of Reproductive Endocrinology Zhejiang Provincial People\u2019s Hospital (Affiliated People\u2019s Hospital, Hangzhou Medical College)  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Zhang","sequence":"additional","affiliation":[{"name":"Center for Reproductive Medicine, Department of Reproductive Endocrinology Zhejiang Provincial People\u2019s Hospital (Affiliated People\u2019s Hospital, Hangzhou Medical College)  Hangzhou China"},{"name":"Department of Obstetrics Zhejiang Provincial People\u2019s Hospital, (Affiliated People\u2019s Hospital, Hangzhou Medical College)  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lijia","family":"Ma","sequence":"additional","affiliation":[{"name":"School of Life Sciences Westlake University  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0398-2188","authenticated-orcid":false,"given":"Jing","family":"Shu","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Qingdao Medical College of Qingdao University  Qingdao China"},{"name":"Center for Reproductive Medicine, Department of Reproductive Endocrinology Zhejiang Provincial People\u2019s Hospital (Affiliated People\u2019s Hospital, Hangzhou Medical College)  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,2,24]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/humupd\/dmv016"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2018.06.005"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-0378(98)00017-5"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/17.1.213"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/humupd\/dml004"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2018.07.027"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/jog.12392"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mce.2011.10.028"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0020-7292(02)00341-7"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/humupd\/dmg045"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-0032-1315059"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/dex312"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2017.10.005"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/deg461"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1177\/1933719117698580"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.4103\/jhrs.JHRS_9_17"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2016.12.005"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1093\/humupd\/4.5.448"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0015-0282(99)00484-7"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0015-0282(01)03276-9"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.rbmo.2016.05.002"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2014.07.447"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40618-018-0877-1"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tjog.2018.10.007"},{"key":"e_1_2_11_26_1","first-page":"575","article-title":"Efficacy of gamma\u2010irradiated adipose\u2010derived stem cells for treatment of thin endometrium in rats","volume":"37","author":"Ye MX","year":"2017","journal-title":"Nan Fang Yi Ke Da Xue Xue Bao"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1093\/biolre\/ioy175"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2008.01.029"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-0043-111899"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.1080\/09513590.2018.1554035"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccell.2020.09.015"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2017.05.035"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2018.05.057"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41591-020-1040-z"},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41588-021-00972-2"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.4402"},{"key":"e_1_2_11_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexcr.2020.112153"},{"key":"e_1_2_11_38_1","doi-asserted-by":"publisher","DOI":"10.1093\/molehr\/gaaa058"},{"key":"e_1_2_11_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0008-6363(97)00039-4"},{"key":"e_1_2_11_40_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1501859"},{"key":"e_1_2_11_41_1","doi-asserted-by":"publisher","DOI":"10.1634\/stemcells.19-4-304"},{"key":"e_1_2_11_42_1","doi-asserted-by":"publisher","DOI":"10.1038\/339312a0"},{"key":"e_1_2_11_43_1","doi-asserted-by":"publisher","DOI":"10.26508\/lsa.202000935"},{"key":"e_1_2_11_44_1","doi-asserted-by":"publisher","DOI":"10.1189\/jlb.0806510"},{"key":"e_1_2_11_45_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00418-005-0137-2"},{"key":"e_1_2_11_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2019.05.031"},{"key":"e_1_2_11_47_1","volume-title":"Female Sex Steroid Hormone Regulation of Cell Proliferation in the Endometrium","author":"Aplin JD","year":"2008"},{"key":"e_1_2_11_48_1","doi-asserted-by":"publisher","DOI":"10.1038\/cddis.2013.262"},{"key":"e_1_2_11_49_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40618-020-01455-7"},{"key":"e_1_2_11_50_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.19.3.2251"},{"key":"e_1_2_11_51_1","doi-asserted-by":"publisher","DOI":"10.1002\/rmb2.12030"},{"key":"e_1_2_11_52_1","doi-asserted-by":"publisher","DOI":"10.1093\/molehr\/gal019"},{"key":"e_1_2_11_53_1","doi-asserted-by":"publisher","DOI":"10.1159\/000066970"},{"key":"e_1_2_11_54_1","first-page":"1885\u20101","article-title":"Integrin beta8 (ITGB8) activates VAV\u2010RAC1 signaling via FAK in the acquisition of endometrial epithelial cell receptivity for blastocyst implantation","volume":"7","author":"Kumar V","year":"2017","journal-title":"Sci Rep"},{"key":"e_1_2_11_55_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12958-018-0379-1"},{"key":"e_1_2_11_56_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/dew143"},{"key":"e_1_2_11_57_1","doi-asserted-by":"publisher","DOI":"10.1210\/er.2003-0020"},{"key":"e_1_2_11_58_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI1597"},{"key":"e_1_2_11_59_1","doi-asserted-by":"publisher","DOI":"10.1210\/mend.13.6.0284"},{"key":"e_1_2_11_60_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.10-175349"}],"container-title":["The FASEB Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1096\/fj.202101579R","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1096\/fj.202101579R","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/faseb.onlinelibrary.wiley.com\/doi\/pdf\/10.1096\/fj.202101579R","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T18:59:54Z","timestamp":1761677994000},"score":1,"resource":{"primary":{"URL":"https:\/\/faseb.onlinelibrary.wiley.com\/doi\/10.1096\/fj.202101579R"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,2,24]]},"references-count":59,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2022,3]]}},"alternative-id":["10.1096\/fj.202101579R"],"URL":"https:\/\/doi.org\/10.1096\/fj.202101579r","archive":["Portico"],"relation":{"has-review":[{"id-type":"doi","id":"10.3410\/f.741722216.793592117","asserted-by":"object"}]},"ISSN":["0892-6638","1530-6860"],"issn-type":[{"value":"0892-6638","type":"print"},{"value":"1530-6860","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,2,24]]},"assertion":[{"value":"2021-10-12","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-01-18","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-02-24","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e22193"}}