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To clarify the expression patterns of markers specific for these lineages in bovine embryos, we analyzed day 7, 9, and 12 blastocysts completely produced in vivo by staining for OCT4, NANOG, SOX2 (EPI), and GATA6, SOX17 (HB) and identified genes specific for these developmental stages in a global transcriptomics approach. To study the role of OCT4, we generated OCT4\u2010deficient (\n                    <jats:italic>OCT4<\/jats:italic>\n                    \u00a0KO) embryos via somatic cell nuclear transfer or in vitro fertilization.\n                    <jats:italic>OCT4<\/jats:italic>\n                    \u00a0KO embryos reached the expanded blastocyst stage by day 8 but lost NANOG and SOX17 expression, while SOX2 and GATA6 were unaffected. Blastocysts transferred to recipient cows from day 6 to 9 expanded, but the\n                    <jats:italic>OCT4<\/jats:italic>\n                    \u00a0KO phenotype was not rescued by the uterine environment. Exposure of\n                    <jats:italic>OCT4<\/jats:italic>\n                    \u00a0KO embryos to exogenous FGF4 or chimeric complementation with\n                    <jats:italic>OCT4<\/jats:italic>\n                    intact embryos did not restore NANOG or SOX17 in OCT4\u2010deficient cells. Our data show that OCT4 is required cell autonomously for the maintenance of pluripotency of the EPI and differentiation of the HB in bovine embryos.\n                  <\/jats:p>","DOI":"10.1096\/fj.202101713rrr","type":"journal-article","created":{"date-parts":[[2022,4,29]],"date-time":"2022-04-29T10:03:07Z","timestamp":1651226587000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["OCT4\/POU5F1 is indispensable for the lineage differentiation of the inner cell mass in bovine embryos"],"prefix":"10.1096","volume":"36","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2188-3108","authenticated-orcid":false,"given":"Kilian","family":"Simmet","sequence":"first","affiliation":[{"name":"Gene Center Department of Veterinary Sciences Institute of Molecular Animal Breeding and Biotechnology LMU Munich  Munich Germany"},{"name":"Center for Innovative Medical Models (CiMM) LMU Munich  Oberschlei\u00dfheim 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