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Here, we have investigated the effect of A\n                    <jats:sub>2A<\/jats:sub>\n                    and A\n                    <jats:sub>2B<\/jats:sub>\n                    \u2010selective agonists on vasodilatation in three distinct vascular beds of the rat cardiovascular system. NanoBRET ligand binding studies were used to confirm receptor selectivity. The regional hemodynamic effects of adenosine A\n                    <jats:sub>2A<\/jats:sub>\n                    and A\n                    <jats:sub>2B<\/jats:sub>\n                    selective agonists were investigated in conscious rats. Male Sprague\u2010Dawley rats (350\u2013450\u00a0g) were chronically implanted with pulsed Doppler flow probes on the renal artery, mesenteric artery, and the descending abdominal aorta. Cardiovascular responses were measured following intravenous infusion (3\u00a0min for each dose) of the A\n                    <jats:sub>2A<\/jats:sub>\n                    \u2010selective agonist CGS 21680 (0.1, 0.3, 1 \u00b5g kg\n                    <jats:sup>\u22121<\/jats:sup>\n                    min\n                    <jats:sup>\u22121<\/jats:sup>\n                    ) or the A\n                    <jats:sub>2B<\/jats:sub>\n                    \u2010selective agonist BAY 60\u20106583 (4,13.3, 40 \u00b5g kg\n                    <jats:sup>\u22121<\/jats:sup>\n                    min\n                    <jats:sup>\u22121<\/jats:sup>\n                    ) following predosing with the A\n                    <jats:sub>2A<\/jats:sub>\n                    \u2010selective antagonist SCH 58261 (0.1 or 1\u00a0mg kg\n                    <jats:sup>\u22121<\/jats:sup>\n                    min\n                    <jats:sup>\u22121<\/jats:sup>\n                    ), the A\n                    <jats:sub>2B<\/jats:sub>\n                    \/A\n                    <jats:sub>2A<\/jats:sub>\n                    antagonist PSB 1115 (10\u00a0mg kg\n                    <jats:sup>\u22121<\/jats:sup>\n                    min\n                    <jats:sup>\u22121<\/jats:sup>\n                    ) or vehicle. The A\n                    <jats:sub>2A<\/jats:sub>\n                    \u2010selective agonist CGS 21680 produced a striking increase in heart rate (HR) and hindquarters vascular conductance (VC) that was accompanied by a significant decrease in mean arterial pressure (MAP) in conscious rats. In marked contrast, the A\n                    <jats:sub>2B<\/jats:sub>\n                    \u2010selective agonist BAY 60\u20106583\u00a0significantly increased HR and VC in the renal and mesenteric vascular beds, but not in the hindquarters. Taken together, these data indicate that A\n                    <jats:sub>2A<\/jats:sub>\n                    and A\n                    <jats:sub>2B<\/jats:sub>\n                    receptors are regionally selective in their regulation of vascular tone. These results suggest that the development of A\n                    <jats:sub>2B<\/jats:sub>\n                    receptor agonists to induce vasodilatation in the kidney may provide a good therapeutic approach for the treatment of acute kidney injury.\n                  <\/jats:p>","DOI":"10.1096\/fj.202101945r","type":"journal-article","created":{"date-parts":[[2022,3,1]],"date-time":"2022-03-01T09:30:04Z","timestamp":1646127004000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Regionally selective cardiovascular responses to adenosine A\n                    <sub>2A<\/sub>\n                    and A\n                    <sub>2B<\/sub>\n                    receptor activation"],"prefix":"10.1096","volume":"36","author":[{"given":"Samantha L.","family":"Cooper","sequence":"first","affiliation":[{"name":"Division of Physiology, Pharmacology and Neuroscience School of Life Sciences University of Nottingham  Nottingham UK"},{"name":"Centre of Membrane Proteins and Receptors University of Birmingham and University of Nottingham  Midlands UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edward S.","family":"Wragg","sequence":"additional","affiliation":[{"name":"Division of Physiology, Pharmacology and Neuroscience School of Life Sciences University of Nottingham  Nottingham UK"},{"name":"Centre of Membrane Proteins and Receptors University of Birmingham and University of Nottingham  Midlands UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrizia","family":"Pannucci","sequence":"additional","affiliation":[{"name":"Division of Physiology, Pharmacology and Neuroscience School of Life Sciences University of Nottingham  Nottingham UK"},{"name":"Centre of Membrane Proteins and Receptors University of Birmingham and University of Nottingham  Midlands UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark","family":"Soave","sequence":"additional","affiliation":[{"name":"Division of Physiology, Pharmacology and Neuroscience School of Life Sciences University of Nottingham  Nottingham UK"},{"name":"Centre of Membrane Proteins and Receptors University of Birmingham and University of Nottingham  Midlands UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4424-239X","authenticated-orcid":false,"given":"Stephen J.","family":"Hill","sequence":"additional","affiliation":[{"name":"Division of Physiology, Pharmacology and Neuroscience School of Life Sciences University of Nottingham  Nottingham UK"},{"name":"Centre of Membrane Proteins and Receptors University of Birmingham and University of Nottingham  Midlands UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5406-6847","authenticated-orcid":false,"given":"Jeanette","family":"Woolard","sequence":"additional","affiliation":[{"name":"Division of Physiology, Pharmacology and Neuroscience School of Life Sciences University of Nottingham  Nottingham UK"},{"name":"Centre of Membrane Proteins and Receptors University of Birmingham and University of Nottingham  Midlands 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