{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T12:08:29Z","timestamp":1792757309448,"version":"4.5.0"},"reference-count":50,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2022,10,31]],"date-time":"2022-10-31T00:00:00Z","timestamp":1667174400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82071142"],"award-info":[{"award-number":["82071142"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82071119"],"award-info":[{"award-number":["82071119"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2022,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Mechanical stress regulates various cellular functions like cell inflammation, immune responses, proliferation, and differentiation to maintain tissue homeostasis. However, the impact of mechanical signals on macrophages and the underlying mechanisms by which mechanical force regulates bone remodeling during orthodontic tooth movement remain unclear. NOD\u2010like receptor family pyrin domain\u2010containing 3 (NLRP3) inflammasome has been reported to promote osteoclastic differentiation to regulate alveolar bone resorption. But the relationship between the compressive force and NLRP3 inflammasome in macrophages remains unknown. In this study, immunohistochemical staining results showed elevated expression of NLRP3 and interleukin\u20101\u03b2, as well as an increased number of macrophages expressing NLRP3, on the compression side of the periodontal tissues, after force application for 7\u2009days. Furthermore, the number of tartrate\u2010resistant acid phosphatase\u2010positive osteoclasts, and the mRNA and protein expression levels of osteoclast\u2010related genes in the periodontal tissue decreased in the\n                    <jats:italic>Nlrp3<\/jats:italic>\n                    <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    mice compared to the WT mice group after orthodontic movement. In vitro mechanical force activates the NLRP3 inflammasome and inhibits autophagy. Intraperitoneal injection of the autophagy inhibitor 3\u2010methyladenine in\n                    <jats:italic>Nlrp3<\/jats:italic>\n                    <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    mice promoted orthodontic tooth movement. This result indicates that the absence of NLRP3 inflammasome activation can be partially compensated for by autophagy inhibitors. Mechanistically, force\u2010induced activation of the NLRP3 inflammasome in macrophages via the cGAS\/P2X7R axis. In conclusion, compressive force regulates orthodontic tooth movement via activating the NLRP3 inflammasome.\n                  <\/jats:p>","DOI":"10.1096\/fj.202200447rr","type":"journal-article","created":{"date-parts":[[2022,10,31]],"date-time":"2022-10-31T07:16:10Z","timestamp":1667200570000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":27,"title":["Compressive force regulates orthodontic tooth movement via activating the\n                    <scp>NLRP3<\/scp>\n                    inflammasome"],"prefix":"10.1096","volume":"36","author":[{"given":"Yineng","family":"Han","sequence":"first","affiliation":[{"name":"Department of Orthodontics Peking University School and Hospital of Stomatology  Beijing People's Republic of China"},{"name":"Key Laboratory of Oral Biomedical Research of Zhejiang Province, Stomatology Hospital, School of Stomatology Zhejiang University School of Medicine, Zhejiang Provincial Clinical Research Center for Oral Diseases  Hangzhou People's Republic of China"},{"name":"National Center of Stomatology, National Clinical Research Center for Oral Diseases, National Engineering Laboratory for Digital and Material Technology of Stomatology  Beijing People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiaolin","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Orthodontics Peking University School and Hospital of Stomatology  Beijing People's Republic of China"},{"name":"National Center of Stomatology, National Clinical Research Center for Oral Diseases, National Engineering Laboratory for Digital and Material Technology of Stomatology  Beijing People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yiping","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Orthodontics Peking University School and Hospital of Stomatology  Beijing People's Republic of China"},{"name":"National Center of Stomatology, National Clinical Research Center for Oral Diseases, National Engineering Laboratory for Digital and Material Technology of Stomatology  Beijing People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pengfei","family":"Gao","sequence":"additional","affiliation":[{"name":"Key Laboratory of Cell Proliferation and Differentiation of the Ministry of Education, School of Life Sciences Peking University  Beijing People's Republic of China"},{"name":"Peking\u2010Tsinghua Center for Life Sciences  Beijing People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lingfei","family":"Jia","sequence":"additional","affiliation":[{"name":"National Center of Stomatology, National Clinical Research Center for Oral Diseases, National Engineering Laboratory for Digital and Material Technology of Stomatology  Beijing People's Republic of China"},{"name":"Department of Oral and Maxillofacial Surgery Peking University School and Hospital of Stomatology  Beijing People's Republic of China"},{"name":"Central Laboratory Peking University School and Hospital of Stomatology  Beijing People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1899-8051","authenticated-orcid":false,"given":"Yunfei","family":"Zheng","sequence":"additional","affiliation":[{"name":"Department of Orthodontics Peking University School and Hospital of Stomatology  Beijing People's Republic of China"},{"name":"National Center of Stomatology, National Clinical Research Center for Oral Diseases, National Engineering Laboratory for Digital and Material Technology of Stomatology  Beijing People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9895-1143","authenticated-orcid":false,"given":"Weiran","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Orthodontics Peking University School and Hospital of Stomatology  Beijing People's Republic of China"},{"name":"National Center of Stomatology, National Clinical Research Center for Oral Diseases, National Engineering Laboratory for Digital and Material Technology of Stomatology  Beijing People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,10,31]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41556-017-0005-z"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.3892\/mmr.2015.4141"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bone.2007.07.019"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.3892\/ijmm.2012.1220"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbadis.2015.05.010"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1177\/0022034515589714"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1177\/0022034515595003"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0757.2000.2240104.x"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0765.2011.01351.x"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/jre.12109"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.200737638"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.4248\/BR201301003"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12016-015-8519-2"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13287-020-01607-9"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/cpr.12912"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2100225118"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2017.09.039"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/s1097-2765(00)80032-5"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/s1097-2765(02)00599-3"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1002720"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1177\/0022034520933533"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1186\/s41232-019-0092-2"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbiomech.2012.09.006"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/odi.13637"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2021.666631"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/JPER.19-0049"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2010.01.028"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1184429"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajodo.2008.10.018"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2013.09.010"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.1111\/cpr.12973"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.11944"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexcr.2019.111745"},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.1177\/154405910408300803"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbiosc.2015.02.006"},{"key":"e_1_2_11_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gene.2015.03.055"},{"key":"e_1_2_11_38_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41580-020-0244-x"},{"key":"e_1_2_11_39_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1402658"},{"key":"e_1_2_11_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2017.06.020"},{"key":"e_1_2_11_41_1","doi-asserted-by":"publisher","DOI":"10.3389\/fncel.2017.00227"},{"key":"e_1_2_11_42_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri.2016.100"},{"key":"e_1_2_11_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajodo.2020.09.034"},{"key":"e_1_2_11_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajodo.2020.01.027"},{"key":"e_1_2_11_45_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.202101589R"},{"key":"e_1_2_11_46_1","doi-asserted-by":"publisher","DOI":"10.1186\/ar4365"},{"key":"e_1_2_11_47_1","doi-asserted-by":"publisher","DOI":"10.1186\/ar4365"},{"key":"e_1_2_11_48_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnagi.2018.00116"},{"key":"e_1_2_11_49_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2018.02563"},{"key":"e_1_2_11_50_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1400653"},{"key":"e_1_2_11_51_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1414859111"}],"container-title":["The FASEB Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1096\/fj.202200447RR","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1096\/fj.202200447RR","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/faseb.onlinelibrary.wiley.com\/doi\/pdf\/10.1096\/fj.202200447RR","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T01:16:21Z","timestamp":1761700581000},"score":1,"resource":{"primary":{"URL":"https:\/\/faseb.onlinelibrary.wiley.com\/doi\/10.1096\/fj.202200447RR"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,10,31]]},"references-count":50,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2022,12]]}},"alternative-id":["10.1096\/fj.202200447RR"],"URL":"https:\/\/doi.org\/10.1096\/fj.202200447rr","archive":["Portico"],"relation":{},"ISSN":["0892-6638","1530-6860"],"issn-type":[{"value":"0892-6638","type":"print"},{"value":"1530-6860","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,10,31]]},"assertion":[{"value":"2022-03-23","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-10-12","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-10-31","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e22627"}}