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Nuclear factor erythroid2\u2010related factor2 (Nrf2), a transcriptional activator, enhances its ability to resist oxidative stress by activating multiple downstream anti\u2010oxidants, anti\u2010inflammatory proteins, and detoxifying enzymes. However, the mechanism of Nrf2 signaling in HG\u2010induced DCM is unclear. In this study, we used HG pretreated H9c2 cells as the experimental basis in vitro, and established a high fat\u2010diet, streptozotocin (STZ) induced Type 2 diabetic rat model in vivo. Meanwhile, we used shRNA\u2010Nrf2 and curcumin (CUR) (as an activator) to affect H9c2 cells, to verify the role of the Nrf2 signaling pathway in DCM. The results showed that the excessive production of ROS caused by HG, which could inhibit the activation of Nrf2\u2010related signaling, resulting in a decrease in cell energy metabolism and an increase in cell apoptosis. Surprisingly, we found that the activation of the Nrf2 signaling pathway significantly increased cardiomyocyte viability, reduced ROS formation, increased antioxidant enzyme activity, and inhibited cardiomyocyte apoptosis. In conclusion, these findings conclusively infer that CUR activation of the Nrf2\/HO\u20101 signaling pathway exerts myocardial protection by reducing ROS formation.<\/jats:p>","DOI":"10.1096\/fj.202200543rrr","type":"journal-article","created":{"date-parts":[[2022,8,16]],"date-time":"2022-08-16T03:45:07Z","timestamp":1660621507000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":63,"title":["Curcumin activates Nrf2\/\n                    <scp>HO<\/scp>\n                    \u20101 signaling to relieve diabetic cardiomyopathy injury by reducing\n                    <scp>ROS<\/scp>\n                    in vitro and in vivo"],"prefix":"10.1096","volume":"36","author":[{"given":"Xia","family":"Wu","sequence":"first","affiliation":[{"name":"Department of Pharmacology, Nanjing First Hospital Nanjing Medical University  Nanjing 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