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Using a mouse model lacking\n                    <jats:italic>Adgra3<\/jats:italic>\n                    (\n                    <jats:italic>Adgra3<\/jats:italic>\n                    <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    ), we show that 55% of the male mice are infertile from puberty despite having normal spermatogenesis and epididymal sperm count. Instead, male mice lacking\n                    <jats:italic>Adgra3<\/jats:italic>\n                    exhibited decreased estrogen receptor alpha expression and transient dilation of the epididymis. Combined with an increased estradiol production, this indicates a post\u2010pubertal hormonal imbalance and fluid retention. Dye injection revealed a blockage between the ejaculatory duct and the urethra, which is rare in mice suffering from infertility, thereby mimicking the etiologies of obstructive azoospermia found in human male infertility. To summarize, male reproductive tract development is dependent on ADGRA3 function that in concert with estrogen signaling may influence fluid handling during sperm maturation and storage.\n                  <\/jats:p>","DOI":"10.1096\/fj.202200762rr","type":"journal-article","created":{"date-parts":[[2023,1,23]],"date-time":"2023-01-23T09:43:30Z","timestamp":1674467010000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Loss of\n                    <i>Adgra3<\/i>\n                    causes obstructive azoospermia with high penetrance in male mice"],"prefix":"10.1096","volume":"37","author":[{"given":"Maja L.","family":"Nybo","sequence":"first","affiliation":[{"name":"Laboratory for Molecular Pharmacology, Department of Biomedical Sciences, Faculty of Health and Medical Sciences University of Copenhagen  Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jone M.","family":"Kvam","sequence":"additional","affiliation":[{"name":"Laboratory for Molecular Pharmacology, Department of Biomedical Sciences, Faculty of Health and Medical Sciences University of Copenhagen  Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John E.","family":"Nielsen","sequence":"additional","affiliation":[{"name":"Department of Growth and Reproduction and International Center for Research and Research Training in Endocrine Disruption of Male Reproduction and Child Health (EDMaRC) Copenhagen University Hospital \u2013 Rigshospitalet  Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hanne","family":"Frederiksen","sequence":"additional","affiliation":[{"name":"Department of Growth and Reproduction and International Center for Research and Research Training in Endocrine Disruption of Male Reproduction and Child Health (EDMaRC) Copenhagen University Hospital \u2013 Rigshospitalet  Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katja","family":"Spiess","sequence":"additional","affiliation":[{"name":"Laboratory for Molecular Pharmacology, Department of Biomedical Sciences, Faculty of Health and Medical Sciences University of Copenhagen  Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kristian H. 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