{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T09:53:26Z","timestamp":1791194006266,"version":"4.1.0"},"reference-count":35,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2022,12,5]],"date-time":"2022-12-05T00:00:00Z","timestamp":1670198400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2022,12,5]],"date-time":"2022-12-05T00:00:00Z","timestamp":1670198400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2023,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Peritoneal fibrosis (PF) is an irreversible complication of peritoneal dialysis (PD) that leads to loss of peritoneal membrane function. We investigated PD effluent and serum levels and the tissue expression of chemokine (C\u2010C motif) ligand 8 (CCL8) in patients with PD. Additionally, we investigated their association with PF in a mouse model. Eighty\u2010two end\u2010stage renal disease (ESRD) patients with PD were examined. CCL8 levels were measured via enzyme\u2010linked immunosorbent assays in PD effluents and serum and analyzed with peritoneal transport parameters. Human peritoneal mesothelial cells (hPMCs) were obtained from the PD effluents of 20 patients. Primary cultured hPMCs were treated with recombinant (r) transforming growth factor (TGF)\u2010\u03b2, and CCL8 expression was assessed via western blotting. As the duration of PD increased, the concentration of CCL8 in PD effluents significantly increased. Correlations between peritoneal transport parameters and dialysate CCL8 levels were observed. Western blotting analysis showed that CCL8 was upregulated via rTGF\u2010\u03b2 treatment, accompanied by increases in markers of inflammation, fibrosis, senescence, and apoptosis in hPMCs after induction of fibrosis with rTGF\u2010\u03b2. Anti\u2010CCL8 monoclonal antibody (mAb) treatment suppressed the rTGF\u2010\u03b2\u2010induced increase in all analyzed markers. Immunohistochemical analysis revealed that CCL8 along with fibrosis\u2010 and inflammation\u2010related markers were significantly increased in the PF mouse model. Functional blockade of CCL8 using a CCR8 inhibitor (R243) abrogated peritoneal inflammation and fibrosis in vivo. In conclusion, high CCL8 levels in PD effluents may be associated with an increased risk of PD failure, and the CCL8 pathway is associated with PF. CCL8 blockade can ameliorate peritoneal inflammation and fibrosis.<\/jats:p>","DOI":"10.1096\/fj.202200784r","type":"journal-article","created":{"date-parts":[[2022,12,5]],"date-time":"2022-12-05T08:46:59Z","timestamp":1670230019000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Inflammatory chemokine (\n                    <scp>C\u2010C<\/scp>\n                    motif) ligand 8 inhibition ameliorates peritoneal fibrosis"],"prefix":"10.1096","volume":"37","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9216-420X","authenticated-orcid":false,"given":"Yeonhee","family":"Lee","sequence":"first","affiliation":[{"name":"Department of Internal Medicine, Uijeongbu Eulji Medical Center Eulji University  Gyeonggi\u2010do Republic of Korea"},{"name":"Department of Internal Medicine Seoul National University College of Medicine  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jangwook","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine Dongguk University Ilsan Hospital  Gyeonggi\u2010do Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Minkyoung","family":"Park","sequence":"additional","affiliation":[{"name":"Kidney Research Institute Seoul National University  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Areum","family":"Seo","sequence":"additional","affiliation":[{"name":"Kidney Research Institute Seoul National University  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kyu Hyeon","family":"Kim","sequence":"additional","affiliation":[{"name":"Kidney Research Institute Seoul National University  Seoul Republic of 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Medicine  Ulsan Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sunhwa","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine Kangwon National University Hospital, Kangwon National University School of Medicine  Chuncheon Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dong Ki","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine Seoul National University College of Medicine  Seoul Republic of Korea"},{"name":"Kidney Research Institute Seoul National University  Seoul Republic of Korea"},{"name":"Biomedical Research Institute Seoul National University Hospital  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kook\u2010Hwan","family":"Oh","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine Seoul National University College of Medicine  Seoul Republic of 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