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However, the functions of most of these genes in reproduction are unclear. Here, we showed that mouse\n                    <jats:italic>4930590J08Rik<\/jats:italic>\n                    was a testis\u2010specifically expressed gene.\n                    <jats:italic>4930590J08Rik<\/jats:italic>\n                    knockout mice exhibited a delay in the first wave of spermatogenesis and a reduction of cauda epididymal sperm. Furthermore, knockout spermatozoa exhibited defective acrosome reactions and decreased progressive motility, which led to impaired in vivo fertilization. Transcriptome analysis of testes revealed that most of the differentially expressed genes in knockout testes were associated with metabolic processes.\n                    <jats:italic>4930590J08Rik<\/jats:italic>\n                    knockout sperm exhibited oxidative phosphorylation deficiency and were highly dependent on increased anaerobic glycolysis to compensate for ATP demands. Taken together, the\n                    <jats:italic>4930590J08Rik<\/jats:italic>\n                    \u2010disrupted mouse partially mimics the phenotypes of human asthenospermia and oligozoospermia, which provides a new model for further understanding the pathogenesis of idiopathic male infertility.\n                  <\/jats:p>","DOI":"10.1096\/fj.202200805rr","type":"journal-article","created":{"date-parts":[[2022,11,4]],"date-time":"2022-11-04T12:13:34Z","timestamp":1667564014000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Impaired fertility in\n                    <scp>\n                      <i>4930590J08Rik<\/i>\n                    <\/scp>\n                    mutant male mice is associated with defective sperm energy metabolism"],"prefix":"10.1096","volume":"36","author":[{"given":"Rui","family":"Chen","sequence":"first","affiliation":[{"name":"College of Life Science and Technology Huazhong 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