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It was perfused using anionic (0.5% w\/v sodium dodecyl sulfate) as well as non\u2010ionic (0.1% v\/v triton X\u2010100, t\u2010octyl phenoxy polyethoxy ethanol) detergents and washed intermittently with 1XPBS supplemented with 0.1% v\/v antibiotic and nucleases in a gravitation\u2010driven set\u2010up. After 48\u00a0h, a white decellularized pancreas was obtained, and its extracellular matrix (ECM) content was examined for scaffold\u2010like properties. The ECM content was assessed for removal of cellular content, and nuclear material was evaluated with temporal H&amp;E staining. Quantified DNA was found to be present in a negligible amount in the resultant decellularized pancreas tissue (DPT), thus prohibiting it from triggering any immunogenicity. Collagen and fibronectin were confirmed to be preserved upon trichrome and immunohistochemical staining, respectively. SEM and AFM images reveal interconnected collagen fibril networks in the DPT, confirming that collagen was unaffected. sGAG was visualized using Prussian blue staining and quantified with DMMB assay, where DPT has effectively retained this ECM component. Uniaxial tensile analysis revealed that DPT possesses better elasticity than NPT (native pancreatic tissue). Physical parameters like tensile strength, stiffness, biodegradation, and swelling index were retained in the DPT with negligible loss. The cytocompatibility analysis of DPT has shown no cytotoxic effect for up to 72\u00a0h on normal insulin\u2010producing cells (MIN\u20106) and cancerous glioblastoma (LN229) cells in vitro. The scaffold was recellularized using isolated mouse islets, which have established in vitro cell proliferation for up to 9\u2009days. The scaffold received at the end of the decellularization cycle was found to be non\u2010toxic to the cells, retained biological and physical properties of the native ECM, suitable for recellularization, and can be used as a safer and better alternative as a transplantable organ from a xenogeneic source.<\/jats:p>","DOI":"10.1096\/fj.202200807r","type":"journal-article","created":{"date-parts":[[2022,9,27]],"date-time":"2022-09-27T06:32:38Z","timestamp":1664260358000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Establishment of decellularized extracellular matrix scaffold derived from caprine pancreas as a novel alternative template over porcine pancreatic scaffold for prospective biomedical application"],"prefix":"10.1096","volume":"36","author":[{"given":"Garima","family":"Singh","sequence":"first","affiliation":[{"name":"Immunology and Molecular Medicine Laboratory Department of Life Science, National 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