{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T01:43:14Z","timestamp":1790991794798,"version":"4.1.0"},"reference-count":102,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2022,11,14]],"date-time":"2022-11-14T00:00:00Z","timestamp":1668384000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2022,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Traumatic spinal cord injury (tSCI) is a severe injury of the central nervous system (CNS) with complicated pathological microenvironment that results in hemorrhage, inflammation, and scar formation. The microenvironment of the injured spinal cord comprises heterogeneous neurons, glial cells, inflammatory cells, and stroma\u2010related cells. Increasing evidence has indicated that the altered cellular and molecular microenvironment following tSCI is a key factor impeding functional recovery. Single\u2010cell RNA sequencing (scRNA\u2010seq) has provided deep insights into the dynamic cellular and molecular changes in the microenvironment by comprehensively characterizing the diversity of spinal cord cell types. Specifically, scRNA\u2010seq enables the exploration of the molecular mechanisms underlying tSCI by elucidating intercellular communication in spinal cord samples between normal and injury conditions at a single\u2010cell resolution. Here, we first described the pathological and physiological processes after tSCI and gave a brief introduction of the scRNA\u2010seq technology. We then focused on the recent scRNA\u2010seq researches in tSCI, which characterized diverse cell\u2010type populations and specific cell\u2013cell interactions in tSCI. In addition, we also highlighted some potential directions for the research of scRNA\u2010seq in tSCI in the future.<\/jats:p>","DOI":"10.1096\/fj.202200943r","type":"journal-article","created":{"date-parts":[[2022,11,14]],"date-time":"2022-11-14T10:51:08Z","timestamp":1668423068000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Single\u2010cell\n                    <scp>RNA<\/scp>\n                    sequencing for traumatic spinal cord injury"],"prefix":"10.1096","volume":"36","author":[{"given":"Yuqi","family":"Cao","sequence":"first","affiliation":[{"name":"Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education, NMPA Key Laboratory for Research and Evaluation of Tissue Engineering Technology Products, Co\u2010innovation Center of Neuroregeneration Nantong University  Nantong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shunxing","family":"Zhu","sequence":"additional","affiliation":[{"name":"Laboratory Animals Center Nantong University  Nantong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bin","family":"Yu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education, NMPA Key Laboratory for Research and Evaluation of Tissue Engineering Technology Products, Co\u2010innovation Center of Neuroregeneration Nantong University  Nantong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3706-955X","authenticated-orcid":false,"given":"Chun","family":"Yao","sequence":"additional","affiliation":[{"name":"Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education, NMPA Key Laboratory for Research and Evaluation of Tissue Engineering Technology Products, Co\u2010innovation Center of Neuroregeneration Nantong University  Nantong China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,11,14]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrdp.2017.18"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00441-019-03039-1"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.4103\/1673-5374.339475"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/s1474-4422(18)30444-7"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.wneu.2018.02.033"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1159\/000524867"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-020-1999-0"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.abf1230"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41593-020-00770-9"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.1523\/jneurosci.2002-04.2004"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.1093\/neuonc\/noab106"},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/nn.3357"},{"key":"e_1_2_12_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41582-019-0270-5"},{"key":"e_1_2_12_15_1","doi-asserted-by":"publisher","DOI":"10.3171\/2018.9.SPINE18682"},{"key":"e_1_2_12_16_1","doi-asserted-by":"publisher","DOI":"10.1136\/jnnp.21.3.216"},{"key":"e_1_2_12_17_1","doi-asserted-by":"publisher","DOI":"10.1093\/neuros\/nyw080"},{"key":"e_1_2_12_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-018-0649-2"},{"key":"e_1_2_12_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/nn.4541"},{"key":"e_1_2_12_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41591-018-0296-z"},{"key":"e_1_2_12_21_1","doi-asserted-by":"publisher","DOI":"10.1177\/0963689718755778"},{"key":"e_1_2_12_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41392-022-00885-4"},{"key":"e_1_2_12_23_1","doi-asserted-by":"publisher","DOI":"10.1523\/jneurosci.0860-21.2021"},{"key":"e_1_2_12_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-020-2795-6"},{"key":"e_1_2_12_25_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20210040"},{"key":"e_1_2_12_26_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12264-022-00897-8"},{"key":"e_1_2_12_27_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells10081886"},{"key":"e_1_2_12_28_1","doi-asserted-by":"publisher","DOI":"10.4103\/1673-5374.243694"},{"key":"e_1_2_12_29_1","doi-asserted-by":"publisher","DOI":"10.1172\/jci.insight.98254"},{"key":"e_1_2_12_30_1","doi-asserted-by":"publisher","DOI":"10.1002\/(sici)1096-9861(19970120)377:3<443::aid-cne10>3.0.co;2-s"},{"key":"e_1_2_12_31_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnagi.2017.00230"},{"key":"e_1_2_12_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainres.2007.04.005"},{"key":"e_1_2_12_33_1","doi-asserted-by":"publisher","DOI":"10.1006\/exnr.1998.6785"},{"key":"e_1_2_12_34_1","doi-asserted-by":"publisher","DOI":"10.1089\/neu.1999.16.255"},{"key":"e_1_2_12_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jneuroim.2013.04.008"},{"key":"e_1_2_12_36_1","doi-asserted-by":"publisher","DOI":"10.1093\/jnen\/60.7.676"},{"key":"e_1_2_12_37_1","doi-asserted-by":"publisher","DOI":"10.3389\/fncel.2013.00034"},{"key":"e_1_2_12_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.it.2016.06.006"},{"key":"e_1_2_12_39_1","doi-asserted-by":"publisher","DOI":"10.4103\/1673-5374.217354"},{"key":"e_1_2_12_40_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature21029"},{"key":"e_1_2_12_41_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13311-018-0631-6"},{"key":"e_1_2_12_42_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-019-11707-7"},{"key":"e_1_2_12_43_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1203165"},{"key":"e_1_2_12_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2018.02.004"},{"key":"e_1_2_12_45_1","doi-asserted-by":"publisher","DOI":"10.3389\/fncel.2019.00546"},{"key":"e_1_2_12_46_1","doi-asserted-by":"publisher","DOI":"10.3171\/spi.2004.1.3.0322"},{"key":"e_1_2_12_47_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11033-014-3176-8"},{"key":"e_1_2_12_48_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-14-583"},{"key":"e_1_2_12_49_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12864-017-3532-x"},{"key":"e_1_2_12_50_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0072567"},{"key":"e_1_2_12_51_1","doi-asserted-by":"publisher","DOI":"10.3389\/fncel.2021.720271"},{"key":"e_1_2_12_52_1","doi-asserted-by":"publisher","DOI":"10.3389\/fgene.2019.00317"},{"key":"e_1_2_12_53_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.4292"},{"key":"e_1_2_12_54_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.1315"},{"key":"e_1_2_12_55_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2006.85"},{"key":"e_1_2_12_56_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4939-7428-3_9"},{"key":"e_1_2_12_57_1","doi-asserted-by":"publisher","DOI":"10.1093\/molehr\/gaw061"},{"key":"e_1_2_12_58_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature05058"},{"key":"e_1_2_12_59_1","doi-asserted-by":"publisher","DOI":"10.1093\/abbs\/gmaa007"},{"key":"e_1_2_12_60_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2012.08.003"},{"key":"e_1_2_12_61_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2639"},{"key":"e_1_2_12_62_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.2282"},{"key":"e_1_2_12_63_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2015.05.002"},{"key":"e_1_2_12_64_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aam8999"},{"key":"e_1_2_12_65_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41587-020-0469-4"},{"key":"e_1_2_12_66_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2015.05.005"},{"key":"e_1_2_12_67_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2018.12.006"},{"key":"e_1_2_12_68_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41581-019-0232-6"},{"key":"e_1_2_12_69_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2019.11.025"},{"key":"e_1_2_12_70_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41590-021-00922-4"},{"key":"e_1_2_12_71_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aaf1204"},{"key":"e_1_2_12_72_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-58327-6"},{"key":"e_1_2_12_73_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.24040"},{"key":"e_1_2_12_74_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41587-020-0465-8"},{"key":"e_1_2_12_75_1","doi-asserted-by":"publisher","DOI":"10.1681\/asn.2018090912"},{"key":"e_1_2_12_76_1","doi-asserted-by":"publisher","DOI":"10.15252\/embr.202152728"},{"key":"e_1_2_12_77_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41593-020-00795-0"},{"key":"e_1_2_12_78_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.173807"},{"key":"e_1_2_12_79_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-019-1195-2"},{"key":"e_1_2_12_80_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2017.05.018"},{"key":"e_1_2_12_81_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.202200337R"},{"key":"e_1_2_12_82_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10571-021-01159-3"},{"key":"e_1_2_12_83_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41593-018-0141-1"},{"key":"e_1_2_12_84_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aav9776"},{"key":"e_1_2_12_85_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tig.2022.03.016"},{"key":"e_1_2_12_86_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-021-03634-9"},{"key":"e_1_2_12_87_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.1253"},{"key":"e_1_2_12_88_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-019-1049-y"},{"key":"e_1_2_12_89_1","doi-asserted-by":"publisher","DOI":"10.1016\/bs.mie.2016.03.020"},{"key":"e_1_2_12_90_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibtech.2020.05.006"},{"key":"e_1_2_12_91_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aaf2403"},{"key":"e_1_2_12_92_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aaw1219"},{"key":"e_1_2_12_93_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41592-019-0548-y"},{"key":"e_1_2_12_94_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.copbio.2017.02.004"},{"key":"e_1_2_12_95_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2014.01.018"},{"key":"e_1_2_12_96_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms222413623"},{"key":"e_1_2_12_97_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.202200463R"},{"key":"e_1_2_12_98_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-022-31797-0"},{"key":"e_1_2_12_99_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-022-04864-z"},{"key":"e_1_2_12_100_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms23116218"},{"key":"e_1_2_12_101_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11427-020-1930-0"},{"key":"e_1_2_12_102_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41593-021-00801-z"},{"key":"e_1_2_12_103_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2018.02.003"}],"container-title":["The FASEB Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1096\/fj.202200943R","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1096\/fj.202200943R","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/faseb.onlinelibrary.wiley.com\/doi\/pdf\/10.1096\/fj.202200943R","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T01:16:31Z","timestamp":1761700591000},"score":1,"resource":{"primary":{"URL":"https:\/\/faseb.onlinelibrary.wiley.com\/doi\/10.1096\/fj.202200943R"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,11,14]]},"references-count":102,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2022,12]]}},"alternative-id":["10.1096\/fj.202200943R"],"URL":"https:\/\/doi.org\/10.1096\/fj.202200943r","archive":["Portico"],"relation":{},"ISSN":["0892-6638","1530-6860"],"issn-type":[{"value":"0892-6638","type":"print"},{"value":"1530-6860","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,11,14]]},"assertion":[{"value":"2022-06-21","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-11-01","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-11-14","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e22656"}}